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High Voltage Pulse Generator for Electroporation of Cells
Puczok, Václav ; Martiš, Jan (referee) ; Červinka, Dalibor (advisor)
The main goal of this thesis is to design control board for the experimental electroporation device and to develop control firmware. The first chapter of this work focuses on the electroporation phenomenon itself. Behaviour of the cell model in external electrical field is described there as well as simulation and overview of how electroporation affects living tissue. It also explains the main requirements for parameters of the electroporation pulses as well as need for ECG synchronization. Furthermore, some remarks are given about novel high frequency electroporation method, which involves use of nanosecond bipolar high voltage pulse bursts. The second chapter briefly introduces commercial electroporation device called Nanoknife, including control part, power part, and it's limits. The third chapter consists of introduction of the novel experimental electroporation device developed at BUT. Power part of this device is discussed as well. Next chapter focuses on design of the control board for this device and also on description of the particular schematic parts. There is a control algorithm explanation in the fifth chapter of this thesis followed by the brief manual to machine operation.
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Converter for supplying of the power LED diode
Puczok, Václav ; Huták, Petr (referee) ; Patočka, Miroslav (advisor)
The aim of this thesis is to design a grid-powered supply for supplying power LED used in illuminating application. The power supply consists of an input line filter which attenuates conducted high frequency signals (EMI filter), an active rectifier (PFC circuit) to improve the power factor and the main constant output current double-sided forward converter. It thoroughly focuses at design of the converter's power stage, mainly at pulse transformer, switching transistors, output rectifier and output filter. Main converter model was created in the Matlab-Simulink environment in order to simulate behaviour of the current loop. Final product of this work is a fully functional power supply and the main converter's model.
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Converter for supplying of the power LED diode
Puczok, Václav ; Huták, Petr (referee) ; Patočka, Miroslav (advisor)
The aim of this thesis is to design a grid-powered supply for supplying power LED used in illuminating application. The power supply consists of an input line filter which attenuates conducted high frequency signals (EMI filter), an active rectifier (PFC circuit) to improve the power factor and the main constant output current double-sided forward converter. It thoroughly focuses at design of the converter's power stage, mainly at pulse transformer, switching transistors, output rectifier and output filter. Main converter model was created in the Matlab-Simulink environment in order to simulate behaviour of the current loop. Final product of this work is a fully functional power supply and the main converter's model.
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High Voltage Pulse Generator for Electroporation of Cells
Puczok, Václav ; Martiš, Jan (referee) ; Červinka, Dalibor (advisor)
The main goal of this thesis is to design control board for the experimental electroporation device and to develop control firmware. The first chapter of this work focuses on the electroporation phenomenon itself. Behaviour of the cell model in external electrical field is described there as well as simulation and overview of how electroporation affects living tissue. It also explains the main requirements for parameters of the electroporation pulses as well as need for ECG synchronization. Furthermore, some remarks are given about novel high frequency electroporation method, which involves use of nanosecond bipolar high voltage pulse bursts. The second chapter briefly introduces commercial electroporation device called Nanoknife, including control part, power part, and it's limits. The third chapter consists of introduction of the novel experimental electroporation device developed at BUT. Power part of this device is discussed as well. Next chapter focuses on design of the control board for this device and also on description of the particular schematic parts. There is a control algorithm explanation in the fifth chapter of this thesis followed by the brief manual to machine operation.
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