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Detection CD56 antigenic expression of the patient with morfology report LGL in the peripheral blood.
Procházková, Irena ; Fátorová, Ilona (advisor) ; Vrbacký, Filip (referee)
Author: Bc. Irena Procházková Job title: Detekce exprese antigenu CD56 u pacientů s morfologickým nálezem LGL v periferní krvi Diploma work Charles Univerzity in Prague, Faculty of Pharmacy in Hradec Králové, Biological and medical department Field of study: Expert of specialized methods Abstract The goal of this diploma work was gathering evidence to support the theory, that LGL cells, large granular lymphocytes, are identical to the population of NK cells, that are detectable by antigen CD56. Numbers of the CD56+ cells were gained by flow cytometry measuring the expression of antigen CD56 labeled with antibody against CD56. The correlation was reviewed with the numbers of LGL cells, which were found in the periferal blood of the patient by microscopic evaluation with common panoptical staining of blood coat. There were used the absolute counts of detected parameters for this comparison. The leukocytes from the measured blood count of the patients on the analyzer Advia 2120i were used for calculation the absolute counts of LGL cells. In the group of patients with immunodeficiencies and various forms of allergy where established an increased number of CD56+/CD3- cells (NK cells), we performed a correlation with the number of LGL cells defined microscopically. We found a strong correlation betwwen the two...
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Detection CD56 antigenic expression of the patient with morfology report LGL in the peripheral blood.
Procházková, Irena ; Fátorová, Ilona (advisor) ; Vrbacký, Filip (referee)
Author: Bc. Irena Procházková Job title: Detekce exprese antigenu CD56 u pacientů s morfologickým nálezem LGL v periferní krvi Diploma work Charles Univerzity in Prague, Faculty of Pharmacy in Hradec Králové, Biological and medical department Field of study: Expert of specialized methods Abstract The goal of this diploma work was gathering evidence to support the theory, that LGL cells, large granular lymphocytes, are identical to the population of NK cells, that are detectable by antigen CD56. Numbers of the CD56+ cells were gained by flow cytometry measuring the expression of antigen CD56 labeled with antibody against CD56. The correlation was reviewed with the numbers of LGL cells, which were found in the periferal blood of the patient by microscopic evaluation with common panoptical staining of blood coat. There were used the absolute counts of detected parameters for this comparison. The leukocytes from the measured blood count of the patients on the analyzer Advia 2120i were used for calculation the absolute counts of LGL cells. In the group of patients with immunodeficiencies and various forms of allergy where established an increased number of CD56+/CD3- cells (NK cells), we performed a correlation with the number of LGL cells defined microscopically. We found a strong correlation betwwen the two...
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