National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Preparation and application of nanoparticles and nanofibres with natural UV filters
Plachá, Monika ; Skoumalová, Petra (referee) ; Márová, Ivana (advisor)
The presented diploma thesis is focused on preparation of nanoparticles and nanofibres with natural UV filters. Liposomes with encapsulated aqueous, ethanol and lipid extracts were prepared. Nanofibers from PHB containing lipid extract were prepared too. As a part of this work, an overview of natural sources with potential effects as UV filters were introduced. Moreover, nanoparticles and nanofibers and methods of their characterization were described. Size, polydisperse index and colloid stability of prepared nanoparticles were characterized via DLS. In experimental part aqueous, ethanol and lipid extracts were prepared from roasted coffee, green coffee and cascara. These extracts were spectrophotometrically characterized for the content of polyphenols, flavonoids, antioxidant activity, tannins and their SPF. Liposomes and liposomes containing PHB with these extracts were prepared and the encapsulation effectivity, short–term and long–term stability as well as SPF of nanoparticles were determined. Nanofibers from PHB containing lipid extracts were prepared via electrospinning and forcespinning methods. Prepared nanofibers were examined via FTIR–ATR. Antioxidant activity, short–term and long–term stability were determined spectrophotometrically. From selected nanoparticles, emulsions and gels were prepared and their SPF was also determined. Three types of emulsions with the best SPF were selected and tested on volunteers. Sedimentation stability of emulsions was tested by analytical centrifuge. Finally, cytotoxicity of selected nanoparticles and nanofibers was tested via MTT assay using human keratinocytes.
Characterization of some natural substances with antimycotic effect
Plachá, Monika ; Hlaváček, Viliam (referee) ; Márová, Ivana (advisor)
The introduced bachelor thesis is focused on investigation of the effects of natural substances with potential antimycotic effects and characterization of their constituents. As a part of this work, an overview of mycosis, antimycotics and constituents of natural substances were introduced. Moreover, antifungal susceptibility and genus Candida were described. In the end of the theoretical part the liposomes and their characterization were described. In the experimental part general characteristics of some natural substances and antimycotic test were analyzed. Aqueous, ethanol, methanol and DMSO extracts were tested on yeast strain Candida glabrata. Aqueous extract of the cloves, cloves oil and their combination were encapsulated into liposomes. The encapsulation effectivity, long-term stability and their antifungal activity were determined too. Characterization of liposomes was identified by DLS. Finally, application of liposomes to a preparation of pharmaceutical and cosmetic preparations were presented.

See also: similar author names
5 PLACHÁ, Markéta
1 PLACHÁ, Michaela
2 PLACHÁ, Milena
2 Plachá, Marie
5 Plachá, Markéta
2 Plachá, Martina
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