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N.sub.2./sub.O and N.sub.2./sub. emissions, denitrification activity and the size of the denitrifying community in a pasture soil – what is the role of pH?
Čuhel, Jiří ; Šimek, Miloslav ; Laughlin, R.J. ; Chéneby, D. ; Bru, D. ; Philippot, L.
Denitrification and subsequent N emissions are significantly influenced by soil pH. Nevertheless, it is not clear, whether pH controls denitrification only due to the influence on kinetics of denitrification enzymes or also due to the different structure of denitrifiers. The objective of the present study was to compare results from N gas emissions and N.sub.2./sub.O molar ratio from soils with artificially changed pH, with those obtained from potential denitrification, and to find out whether there is a relationship between N fluxes and abundance of denitrifiers. The experimental site was located in a grassland area at Borová Farm near Český Krumlov, Czech Republic, and consisted of 12 plots (3x3 m) amended with KOH solution (pH alkaline), H.sub.2./sub.SO.sub.4./sub. solution (pH acidic) or with no amendments (pH natural). The .sup.15./sup.N gas flux method was used to quantify emissions of N.sub.2./sub.O and N.sub.2./sub. in situ at intervals over 74 H.
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