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Význam lipidů v metabolismu tuků
Kohlíková, E. ; Petr, M. ; Přistoupilová, K. ; Přistoupil, T. I. ; Pelclová, D. ; Žák, J. ; Heyrovský, Michael ; Navrátil, Tomáš
In course of last two years we compared values of 34 parameters, obtained from 11 young active sportsmen given 5 g of creatine (CR) daily during 1 month. Among these parameters were the products of metabolic pathways, the changes of which are connected with the development of atherosclerosis. This holds for the levels of cholesterol (CH), triacylglycerols (TAG) and homocysteine (HoCySH), and for the levels of vitamin B12 and of folates in blood.
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Patch Clamp Technique – The Tool for Ions Transport across Model and Real Biological Membranes
Navrátil, Tomáš ; Jaklová Dytrtová, J. ; Jakl, M. ; Šestáková, Ivana ; Mareček, Vladimír ; Krůšek, Jan
The project is aimed at the preparation and characterization of model and/or real phospholipid bilayers at stabilized phase boundaries enabling studies of transfer of charged and uncharged species between the two phases. The best way for investigation of inorganic ion transfer is provided by using mercury as one of the phases, in view of the large amount of the relevant electrochemical data available, whereas the transfer of more complex species is better studied in the system of an organic and an aqueous phase stabilized by (agar) gel. The results contribute to the understanding of ion transport across real membranes. Polypeptides and peptides capable forming ionic channels and enabling transport of various ions and molecules across the membranes, will be incorporated into the model membranes. The possibilities of transport of selected compounds across the model membrane will be investigated using cell permeable peptides as well.
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The Use of Electrochemical Stripping Methods for Lead and Cadmium Speciation in Soil Solutions
Šestáková, Ivana ; Jaklová Dytrtová, J. ; Jakl, M. ; Navrátil, Tomáš ; Fojta, Miroslav
Soil solution is an aqueous phase of soil which is affected by plant root systems. The soil solution contains nutrients, organic and inorganic soil substances and also exudates – substances released by plant roots (e.g. low molecular weight organic acids). For the bioaccumulation (transport from soil into plants) the free metal ions and labile complexes contents (usually in trace amounts) in soil solution are important. That is why the electrochemical methods using working electrode preconcentration step, i.e. differential pulse anodic stripping voltammetry, anodic stripping square-wave voltammetry and stripping chronopotentiometry with constant current, are applicable. Mentioned methods combined with the hanging mercury drop electrode were tested for the cadmium and lead speciation possibilities in model and also real soil solutions.
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Electrochemical detection of trace amounts of nitrated explosives
Barek, J. ; Fischer, J. ; Vyskočil, V. ; Pecková, K. ; Navrátil, Tomáš ; Josypčuk, Bohdan
Nitrated organic compounds belong to the group of the most frequently used explosives. Detection of low levels of such compounds is very attractive in these days. Due to the easy reducibility of the nitro group, voltammetry seems to be very suitable method for these purposes. For these determinations the following electrodes were used: were used hanging mercury drop electrode, meniscus modified silver solid amalgam electrode, miniaturized paste silver amalgam electrode, paste electrode on the basis of silver amalgam powder and proper organic liquid were used.
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Vliv suprafyziologických dávek kreatinu na homocysteinový metabolismus u sportovců
Kohlíková, E. ; Petr, M. ; Přistoupilová, K. ; Šenholdová, Z. ; Přistoupil, T. I. ; Pelclová, D. ; Žák, J. ; Heyrovský, Michael ; Navrátil, Tomáš
One month´s administration of creatine (CR) in the dose of 5 g/day to 11 young men affected their daily amount of CR, creatinine, and thiodiglycolic acid (TDGA) excreted into urine. The probands were divided into 4 groups, according to the amount of CR found in urine, and of folates and of vitamin B12 determined in blood. The changes of folates and vitamin B12 were mutually reciprocal, as well as muscle mass and body fat. Each group utilized CR as donor of one- and two-carbon (1C and 2C) units by means of homocysteine (HoCySH), folates, and vitamin B12, in different metabolic pathways.
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Interaction of Creatine Metabolism with Three Possible Enzymatic Pathways Using Homocysteine
Přistoupilová, K. ; Navrátil, Tomáš ; Petr, M. ; Šenholdová, Z. ; Přistoupil, T. I. ; Pelclová, D. ; Žák, J. ; Kohlíková, E. ; Heyrovský, Michael
Alternative administration of creatine (CR) and vitamin B12 in food supplements has confirmed our finding that CR is a source of 2C units. They can be utilized in oxidative pathway, an intermediate of which is thiodiglycolic acid (TDGA). 2C units can be used also in other metabolic pathways, in which folates and vitamin B12 coenzymes take part together with homocysteine.
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Participation of electrochemistry in the studies of human metabolism
Navrátil, Tomáš ; Šenholdová, Z. ; Přistoupilová, K. ; Přistoupil, T. I. ; Heyrovský, Michael ; Pelclová, D. ; Žák, J.
The contribution deals with explanation of metabolism of long term supplemented creatine in human organism. This process differs from metabolism of natural endogenous creatine, which is excreted in form of creatinine. Exogenous creatine induces increase of peptide formation, of body mass, and of intracellular fluid.
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The use of prototype of multichannel device for electrochemical detection of DNA hybridization
Brázdilová, P. ; Navrátil, Tomáš ; Josypčuk, Bohdan ; Fojta, Miroslav
The article presents newly constructed 8-channel device, which enables sequential analysis realized on up to 8 different or equal sensors. The device is computer controlled. The screen-printed carbon electrodes (SPCE) were connected to the device. DNA hybridization was detected. DNA present in the sample spontaneously adsorbed on the surface of SPCE and free surface was subsequently blocked using beef serum albumin. The adsorbed DNA was then hybridized with the probe marked with biotin and streptavidin conjugated with enzyme alkaline phosphatase (SALP). This enzyme catalyzes the transformation of electrochemically inactive 1-naphtylphosphateone 1-naphtol, which anodic signal was detected DC voltammetry (DCV).
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