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Influence of cultivation medium composition on metabolism of tobacco plants
Minářů, Kateřina ; Ryšlavá, Helena (advisor) ; Müller, Karel (referee)
Phosphoenolpyruvate carboxylase (EC 4.1.1.31; PEPC) plays many roles in plants; it connects the metabolism of saccharides and amino acids. PEPC is regulated at many levels including phosphorylation of serine residue near N-terminus. The aim was to found out, if the composition of cultivation medium affects the activity of PEPC and metabolically related enzymes such as NADP-malic enzyme (EC 1.1.1.40), pyruvate, phosphate dikinase (EC 2.7.9.1; PPDK) and enzymes participating in nitrogen assimilation, thus nitrate reductase (EC 1.7.1.1; NR), glutamine synthetase (EC 6.3.1.2; GS), glutamate synthase (EC 1.4.1.14; GOGAT), NAD-glutamate dehydrogenase (1.4.1.2; NAD-GDH) and NADP-glutamate dehydrogenase (1.4.1.4; NADP-GDH). Tobacco plants (Nicotiana tabacum L., cv. Petit Havana SR1) were grown in vitro in Murashige-Skoog agar. The effect of the presence of 2% succrose, reduced content of nitrogen compounds or phosphate and limited supply of CO2 was followed. Sucrose added to Murashige-Skoog medium caused the increase of enzyme activity of PEPC, NADP-ME and most of enzymes related to nitrogen uptake and metabolism - NR, GS, GOGAT, NAD-GDH a NADP-GDH. Reduced content of nitrogen compounds and phosphate in cultivation medium decreased activity of all monitored enzymes. Enzyme activities were also affected by the...
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