National Repository of Grey Literature 176 records found  beginprevious96 - 105nextend  jump to record: Search took 0.01 seconds. 
Interaction of phenylpropionic acids with copper
Zemanová, Kamila ; Karlíčková, Jana (advisor) ; Matysová, Ludmila (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Kamila Zemanová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Interaction of phenylpropionic acids with copper Copper is an essential trace element which is essential for our body. It has a significant effect on the correct functioning of important organs and it plays an important role in the transfer of electrons at the major enzymatic pathways as a prostetic group. On the other hand, excess or deficiency of copper in the human body can cause many diseases. Phenylpropionic acids are group of substances which can have antioxidant, anti-inflammatory and anticancer effects. They are involved in the scavenging of free radicals and reactive oxygen species. In this diploma thesis, I tested copper chelating activity of six phenylpropionic acids at different pHs by spectrofotometric methods using a hematoxylin and a bathocuproinedisulfonic acid disodium salt as an indicator. 3-(3,4-dihydroxyphenyl)propionic acid showed the highest chelating potential but only using a hematoxylin. KEYWORDS: Copper, Phenylpropionic acids, Antioxidants, Chelating activity, Hematoxylin, Bathocuproin
Determination of illegal compounds in cosmetics by modern chromatographic methods
Novotná, Aneta ; Khalikova, Maria (advisor) ; Matysová, Ludmila (referee)
Charles University, Faculty of Pharmacy Department of Analytical Chemistry Candidate: Aneta Novotná Supervisor: Dr. Maria Albertovna Khalikova, Ph.D. Consultant: doc. RNDr. Dalibor Šatinský, Ph.D. Title of Graduation Thesis: Determination of illegal substances in cosmetics using modern chromatographic methods This diploma thesis deals with the development of ultra - fast liquid chromatographic method, which will best detect the preservatives used in cosmetics (parabens). The development of the method involves the selection of the best stationary phase and the mobile phase. In the next phase, selecting the appropriate temperature, gradient, flow rate, and before applying real cosmetic samples, validation was required to show that the method is sufficiently accurate and repeatable for this research. This development of the method was performed on the UltiMate ™ 3000 HPLC and UHPLC System, 9 stationary phases from 4 manufacturers (Fortis, Ascentis, Kinetex, Onyx), properties and contents were evaluated. The most suitable stationary phase that separated all 9 substances was Kinetex Biphenyl 100A, 2.6 μm 150 x 4.6 mm. Due to the solubility and properties of the parabens, two mobile phases and one combination thereof (ACN, MEOH) were tested. The acetonitrile-containing mobile phase better separated the...
Interaction of phenylpropionic acids with copper
Zemanová, Kamila ; Karlíčková, Jana (advisor) ; Matysová, Ludmila (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Kamila Zemanová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Interaction of phenylpropionic acids with copper Copper is an essential trace element which is essential for our body. It has a significant effect on the correct functioning of important organs and it plays an important role in the transfer of electrons at the major enzymatic pathways as a prostetic group. On the other hand, excess or deficiency of copper in the human body can cause many diseases. Phenylpropionic acids are group of substances which can have antioxidant, anti-inflammatory and anticancer effects. They are involved in the scavenging of free radicals and reactive oxygen species. In this diploma thesis, I tested copper chelating activity of six phenylpropionic acids at different pHs by spectrofotometric methods using a hematoxylin and a bathocuproinedisulfonic acid disodium salt as an indicator. 3-(3,4-dihydroxyphenyl)propionic acid showed the highest chelating potential but only using a hematoxylin. KEYWORDS: Copper, Phenylpropionic acids, Antioxidants, Chelating activity, Hematoxylin, Bathocuproin
Method development for determination of 8-hydroxy-2-deoxy guanosine in urine for clinical research
Šestáková, Veronika ; Kujovská Krčmová, Lenka (advisor) ; Matysová, Ludmila (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Veronika Šestáková Supervisor: assoc. prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of the Diploma Thesis: Method development for determination of 8-hydroxy-2-deoxy guanosine in urine for clinical research This diploma thesis is based on the method development for determination of 8- hydroxy-2-deoxy guanosine, 8-hydroxyguanosine and creatinine using UHPLC. The aim was to develop optimal conditions for clinical research. Experiments were carried out using UHPLC Nexera with mass spectrometer LCMS- 8030, (Shimadzu, Japan). Two stationary phases were tested. The chromatographic separation was achieved using a Meteoric core C18 BIO 4.6 × 50 mm stationary phase with core-shell particles, particle size 2.7 μm (YMC, Germany) secured with a KrudKatcher Ultra 0.5 μm in-line filtr (Phenomenex, Germany). The used mobile phase consisted of water (pH 3 using acetic acid) and methanol (using formic acid 0.2 mM) in the ratio 90:10 (v/v). The temperature was maintained at 25 řC, a flow rate was set at 0.5 mL/min and 4 µl of sample was injected. After optimization of separation conditions, the method was applied to biological material (urine). The samples were prepared using solid-phase extraction. The method...
Development and validation of UHPLC method for determination of omeprazole in oral suspensions
Reiská, Veronika ; Matysová, Ludmila (advisor) ; Kujovská Krčmová, Lenka (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Anylytical Chemistry Candidate: Veronika Reiská Supervisor: doc. PharmDr. Ludmila Matysová, Ph.D. Title of diploma thesis: Development and validation of UHPLC method for determination of omeprazole in oral suspensions The aim of the diploma thesis was to find out suitable conditions for method which enables to determinate the concentration of omeprazole in oral suspensions. There was used the column Kinetex TM 1.7 µm, C18 100 A, 50 x 2.1 mm, (Phenomenex, USA) for analysis. The mobile phase was 0.025mol/L phosphate buffer and acelonitrile in ratio 74:26. A suitable pH value of 7.6 was chosen. The internal standard method of methylparaben was applied. The whole analysis was detected on the wavelength of 300 nm. After finding of optimal conditions, 6 different samples of oral omeprazole suspensions were measured. The solution of the mobile phase was used for dissolution of samples. The method was validated. Keywords: omeprazole, UHPLC, oral suspensions.
Validation of bioanalytical methods - EU and USA legislations (review)
Hrochová, Zuzana ; Matysová, Ludmila (advisor) ; Kujovská Krčmová, Lenka (referee)
This thesis was aimed of the legal requirements and the comparison of specific requirements for validation of bioanalytical methods resulting from the requirement administration Food and Drug Administration (FDA) in the US and European medicines Agency (EMA) in the European Union. It was found that both agencies based on a different legal basis. FDA is part of the federal system. Publishes its own laws and the regulations which have binding legal force . In contrast, the EMA is a non governmental agency and only serves as an advisory comitte of the European Union. Both agencies have issued their instruction - Guids. The FDA issued a Guidance for Industry - Bioanalytical Method Validation and EMA issued Guidaline on bioanalytical method validation. Both manuals are only as recommendations and They haven't legal lability. Both manuals contain similar informations. Both agencies differ from the arrangement of the chapters and an emphasis on individual issues, but the basic parameters that are discussed and Theky are the same selectivity, carry over, LLOQ and petting of the calibration curve, accuracy and precision of the method, system suitability, and cross validation. There were no fundamental differences between the two instructions, they only differ from the criteria that recognize the individual...
Treatment of biological samples before HPLC analysis - determination of alfa-tocoferol in erythrocyte membrane
Pospíchalová, Naďa ; Solich, Petr (advisor) ; Matysová, Ludmila (referee)
α-Tocopherol is the principal membrane antioxidant in mammalian cells, although antioxidant is not the only mechanism of this vitamin. Many studies consider erytrocyte α-tocopherol levels to be more suitable to assess the tocopherol status of organism than its plasma levels. This thesis presents erytrocyte membranes α-tocopherol HPLC-UV analysis with diferential ultracentrigation and solid phase extraction sample pretreatment. Red cell samples were ultracentrifuged (288 000 × g, 3 minutes, 4 řC) in the presence of butylated hydroxytoluene (BHT), D-mannitol, HEPES and CaCl2. Then α-tocopherol was extracted from erytrocyte membranes by solid phase extraction with n-hexane in the presence of ascorbic acid and tocopherol acetate internal standard. The extract was dissolved in methanol and separated on the monolithic column Chromolith Performance RP-18e (100  4.6 mm) using a 100% methanol as the mobile phase. The absorbance of α-tocopherol was measured at 295 nm wavelength.

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