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On-line derivatization of saccharides in capillary electrophoresis
Mareš, Vít ; Křížek, Tomáš (advisor) ; Coufal, Pavel (referee)
Saccharides chains may suffer from various inaccuracies during their biosy- thesis, that effects their biologic functions. Therefore, they can be used for dia- gnosis of some diseases or quality control in biopharmaceutics. Capillary electro- phoresis has its place in diagnosing these occuring errors. In this thesis a method of on-line derivatization of saccharides in fused-silica capillary with 50 cm in lenght and 50 µm in inner diameter was developed. Gluco- se was used as a model molecule, which was labeled by a fluorescent derivatization reagent, 7-aminonaphthalene-1,3-disulfonic acid. Derivatization of glucose and its separation needed optimalization. First was determined an electrophoretic mo- bility of the derivatization reagent in 0.5; 1.0 and 2.5M acetic acid and 20mM phosphate buffer (pH = 3.5). Both, 2.5M acetic acid and 20mM phosphate buffer, were tested as solvents for 7-aminonaphthalene-1,3-disulfonic acid. The reaction with glucose was the best in acetic acid solution. On the contrary, phosphate buf- fer was found to be the best background electrolyte for the separation. Derivatized glucose was detected by UV detector at 229 nm. Henceforth, the reaction con- ditions were adjusted such as reaction time, voltage and time of its application. Input voltage was applied negative due to the...
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Vaccine hesitancy: The journalistic ethics of reporting on the benefits and risks of vaccination
Mareš, Vít ; Kasík, Pavel (advisor) ; Moravec, Václav (referee)
The bachelor thesis "Vaccine hesitancy: The journalistic ethics of reporting on the benefits and risks of vaccination" examines how journalists perceive their role in reporting on health issues, with particular regard to vaccine hesitancy. The latter became a significant social issue during the COVID-19 pandemic. The theoretical part of the thesis discusses vaccine hesitancy itself, its possible causes, and the influence that news media and the journalists can have on it. The practical part is based on quantitative research using a questionnaire survey among journalists from selected Czech online media. It seeks to identify journalists specialising in health care, and to look for how their perspectives and practices differ from those of journalists in other specializations. Due to the small research sample, the research does not provide representative results, but with the help of open-ended questions it provides insight into certain areas of journalistic work related to vaccine and vaccination reporting.
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Application of project management in construction of wooden building
Mareš, Vít ; Riedl, Marcel (advisor) ; Červený, Luboš (referee)
In my thesis, I applied project management in optimizing activities in the implementation of wooden buildings. I used methods of PERT and CPM, and then compared their contribution to practical use in the implementation of the historic wooden buildings of baroque truss. I suggested classification methodology sorting random effects in the implementation of timber, which will be useful in the practice of project management. Then I confronted estimates project duration calculated according to these methods with real historical truss during construction.
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