National Repository of Grey Literature 69 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Novostavba roubeného rodinného domu
Mareš, Pavel
This thesis deals with the comparison of two construction technologies of timbered buildings in terms of financial and time requirements. It is divided into theoretical and practical parts. In the theoretical part, the history of timbered buildings in our country, the legislative requirements for buildings as of 2022, the division of construction systems and production processes of timbered buildings, traditional and modern technologies, the procedure for designing not only timbered buildings, the financial policy of today and the technical description of the building are described. In the practical part of this work, two implementation documents are prepared according to Decree 499/2006, Annex 13. One implementation documentation is prepared for the technology with dried BSH timbers and the other one for the technology with undried imber. Both documents include a budget with a bill of quantities. These documents, including budgets, serve as a basis for the comparison of the above mentioned technologies in terms of financial and time requirements for construction. The basis for the comparison and implementation documentation is a real order of OK Pyrus, s.r.o. with individual requirements of the investor. On the basis of these input documents, it is evaluated which of the following options is more time and financially advantageous.
Development of the circadian system after systemic potentiation of GABAergic transmission in early ontogenesis
Dušek, Jakub ; Bendová, Zdeňka (advisor) ; Mareš, Pavel (referee)
The circadian system is the evolutionary response of organisms to repetitive geophysical conditions and allows organisms to devote their energy to solving sudden changes. Although the circadian system developed to help organisms survive and thrive in dynamic conditions, system itself is sensitive to external changes. During critical developmental period of the mammalian CNS, the neonatal period, the circadian systém is significantly vulnerable. Benzodiazepines, pharmaceuticals widely prescribed for half a century, interfere with GABAergic transmission, which is critical type of signaling for the functioning of the circadian systém. The purpose of this thesis is to determine whether and how the development and functioning of the circadian system is altered after administration of a drug from the benzodiazepine class, clonazepam, during the critical developmental period. This thesis examines changes in the expression profile of selected genes in the hippocampus, olfactory bulb and frontal cortex of two ontogenetic stages of rats. Results of this work suggest that benzodiazepines administred in the neonatal period disrupt the development of the circadian system and that these changes, particularly in the hippocampus, persist for certain time. Key words: circadian system; circadian rhythms;...
Effects of allopregnanolon on motor functions of young laboratory rat.
Krejčí, Lukáš ; Mareš, Pavel (advisor) ; Valeš, Karel (referee)
Allopregnanolone is a typical representative of neurosteroids. It can be formed by de novo synthesis directly in the CNS or as a metabolite of progesterone. It is an allosteric modulator of GABAA receptors, due to which it has anxiolytic, sedative and anticonvulsant effects. This work investigates the effect of allopregnanolone (ALLO) on the locomotor skills of laboratory rat pups. The results will serve as a reference study for the work of Tereza Košťálová (2020), who investigated the newly discovered neuroactive steroid pregnanolone pyroglutamate (PPG). This exogenous steroid, synthesized at the Institute of Organic Chemistry and Biochemistry of the Academy of Sciences of the Czech Republic (IOCB PRAGUE), offers potential use in the treatment of epilepsy. Our goal was to perform the same battery of motor tests after the application of allopregnanolone as a comparison, to what extent and in what way these two substances acutely affect the motor performance of the rat pup. The theoretical part of the thesis describes the physiological mechanisms of neurosteroids in the human body and their potential in the treatment of serious diseases, especially epilepsy. Next, the ontogenetic development of the rat's motor skills is described and compared to humans. The methodology describes the selection and...
Consequences of perinatal insult on brain excitability in immature and adult rats
Fábera, Petr ; Mareš, Pavel (advisor) ; Pokorný, Jaroslav (referee) ; Ošlejšková, Hana (referee)
Perinatal insult may lead to a permanent impairment of brain function resulting in the development of epilepsy. Status epilepticus (SE) in immature rats leads to hippocampal hyperexcitability. The functional and morphological changes of the hippocampus are similar to those seen in human temporal lobe epilepsy. The excitability may be influenced by adenosine. Adenosine acts its anticonvulsant effect by activation of A1 receptors (A1R). The concentration of adenosine is regulated by adenosine kinase (ADK) present in two isoforms - ADK-L and -S. The main goal of the thesis is to elucidate the changes in A1R and ADK isoforms expression during intact brain development and after SE. A1R agonist 2-chloro-N6- cyclopentyladenosine (CCPA), as well as inhibition of ADK by 5-iodotubercidin (5-ITU), may bolster the anticonvulsant effect, but their action may correspond with the level of A1R and ADK. Hippocampal excitability in immature rats after LiCl-pilocarpine SE was studied by the model of hippocampal afterdischarges (ADs) and correlated with changes of A1R and ADK in the hippocampus. ADs demonstrated significantly decreased hippocampal excitability shortly after SE induction, whereas significant hyperexcitability accompanied by spontaneous seizures in older rats was shown. Increasing ADK-S expression...
Development of callosal interhemispheric connection in laboratory rat
Matternová, Lucie ; Mareš, Pavel (advisor) ; Žiak, Jakub (referee)
Corpus callosum is the main commissure linking cortex of the right and left brain hemisphere. For the first time, the callosal axons are observed during E18, when they cross the midline. In PND 5, most axons reach the contralateral cortex, and at the end of the second postnatal week, the callosal projection neurons and axon terminals are located similarly to the adult animal. The interhemispheric response was first observed in the rat in PND 4 but myelination in PND 12, suggesting that the transcallosal response is not dependent on myelinization of callosal fibers. As the rat age increases, the wave duration of the TCR, latency peak and threshold decreases. As opposed to that, amplitude of the positive and negative wave increases with age. TCR may be affected by acute drug application or chronic exposure to various effects, such as hormones.
The influence of status epilepticus (SE) in postnatal development early phase on laboratory rat psychomotor development
Boťchová, Lenka ; Kubová, Hana (advisor) ; Mareš, Pavel (referee)
Bibliografická identifikace v angličtině Author's first name and surname: Lenka Boťchová, BA Title of the master thesis: Does status epilepticus during early postnatal period influence laboratory rat psychomotor development? Department: Department of Rehabilitation and Exercise Medicine Supervisor: doc. PharmDr. H. Kubová, DrSc. The year of presentation: 2013 Abstract: The purpose of this study is to evaluate the influence of status epilepticus (SE) in postnatal development early phase on laboratory rat psychomotor development. Experimentally caused (SE) is the most common mode of epileptogenesis, which is the process leading to the epilepsy creation, and typical comorbidities. The influence of the early SE was studied when using lithium/pilocarpine model of SE induced on 12 days old rat cubs, which were repeatedly exposed to the "open field" test until 32nd day of their lives that means until the puberty beginning. The evaluation is focused on cognition and overall locomotion changes and anxiety demonstrations. The results show that in short periods after SE (up to 1 month) the cognitive functions regarding the habituation disorder are not worsened. However, hyperactivity short-term disposition and worse adaptability to experimental conditions due to animal anxiety increase were detected. Motor skills of...
Věkové a pohlavní rozdíly v GABAergní transmisi v substantia nigra pars reticulata u krys.
Chudomel, Ondřej ; Bojar, Martin (advisor) ; Vyklický, Ladislav (referee) ; Mareš, Pavel (referee)
Many experimental as well as clinical studies have shown that subcortical neuronal circuitries including the anterior part of the substantia nigra pars reticulata (SNR) are closely involved in the control of seizures propagation and termination. In vivo studies in rats demonstrated that inhibition of the GABAergic SNRA electrical output increases the seizure threshold in the flurothyl model of generalized clonic seizures. The anticonvulsant properties of the SNRA are largely age- and sex-depended. In the current experiments were used 3 age groups of Sprague Dawley rats (PN5-9, PN11-16 and PN25-32 days, PN = postnatal) to study GABAergic inhibition. Two types of GABAergic inhibition were studied: a) a synaptic inhibition, which is generated by α1 and α3-containing GABAARs and b) a tonic inhibition mediated by extrasynaptic δ subunit-containing GABAARs. Immunohistochemistry showed that the α1 subunit expression was generally more abundant in adult rats and females while the α3 subunit dominated in the early development and gradually decreased by the age of PN30. The more α1 subunit was expressed the faster were the kinetics, higher the mean amplitudes and frequencies of spontaneous inhibitory postsynaptic currents (sIPSCs). The α subunit subtype underlies sensitivity to zolpidem, which preferentially...
Extracellular space diffusion parameters and metabolism in the rat somatosrensory cortex during recovery from trabsient global ischemia and hypoxia
Zoremba, Norbert ; Syková, Eva (advisor) ; Mareš, Pavel (referee) ; Rokyta, Richard (referee)
Content Abstract .................................................................. ..... ...... ............................ .... 2 Introduction ............................................ ........................................ .................. 4 Aims .................. ................. ................................ ....... ..... . ............ .......... 6 Methods ............................................... ......................................................... ... 7 Results ................ ............................................................................ .................. 9 Discussion .. ... ............... .. ......... .. .......................... ............................. ... ............. 13 References ....... ................................................................ ....................... ........ 20 List of Publications ............. .... .. ............... .. ... .. .......... .. ... ............................... .... 22 Abstract The extracellular space (ECS) of the brain represents the microenvironment of nerve cells and enables the diffusion of neuroactive substances among neurons, axons and glia. Changes in the ECS diffusion parameters during ischemia are well known, but information about changes in ECS diffusion and energy-related metabolite...
The role of main excitatory and inhibitory transmitters in epileptic seizures and the effect of antiepileptic drugs in the immature brain
Liptáková, Soňa ; Mareš, Pavel (advisor) ; Komárek, Vladimír (referee) ; Šlamberová, Romana (referee)
Epilepsy affects 50 million people worldwide. Approximately 50% of epilepsies start in the infancy and childhood. In addition, there are several age-bound epileptic syndromes which are difficult to treat. Due to these facts, developmental studies of epileptic seizures are of primary importance. Predominance of excitation over inhibition is a mechanism of nearly all types of epileptic seizures. Hypothesis that this compromised balance can be normalized by drugs affecting main excitatory and inhibitory system at different stages of brain development was tested in models with different pathogenesis. Our studies demonstrated that seizures induced by derrangement of GABAergic inhibition can be suppressed by antagonists of NMDA receptors (MK-801) and vice versa seizures with glutamatergic pathogenesis can be affected by drugs potentiating GABAergic system (valproate, ganaxolone) at all developmental stages in immature rats. Analysis of site of action of NMDA receptor antagonists demonstrated reciprocal function of anterior and posterior parts of substantia nigra, structure important for spread of epileptic activity to spinal cord. Special attention was given to an age-dependent model - flexion, emprosthotonic seizures elicited by administration of NMDA. Valproic acid, which potentiates GABAergic inhibition,...

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11 MAREŠ, Pavel
18 MAREŠ, Petr
1 Mareš, Peter
18 Mareš, Petr
1 Mareš, Petr,
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