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Ecotoxicological evaluation of contaminated water and soil and use of biotests for bioremediation
Hubálek, Tomáš ; Novotný, Čeněk (advisor) ; Pazlarová, Jarmila (referee) ; Malachová, Kateřina (referee)
2 2. Abstract The aim of this thesis is to identify the ecotoxicity of contaminated soil and water during bioremediation. Different test organisms were selected for the battery of ecotoxicity tests such as the bacterium Vibrio fischeri, the terrestrial plants Sinapis alba, Lactuca sativa, and Hordeum vulgare, the water plant Lemna minor, the earthworm Eisenia fetida, and the crustaceans Daphnia magna and Heterocypris incongruens. A comparison of individual biotests with various organisms and endpoints should demonstrate their suitability and sensitivity for the ecotoxicity evaluation of contaminated soil and water. The bioassays were used for the evaluation of the bioremediation of hydrocarbon-contaminated soil, composting of PAH-contaminated soil and remediation of contaminated groundwater. The ecotoxicity of hydrocarbon-contaminated soil originating from a brownfield was evaluated during a 17-month biodegradation pilot test (experiment I). The initial concentration of total petroleum hydrocarbons (TPH) in the soil was 6380 μg.g-1 dry weight; the concentration of TPH in the soil decreased by 65.5 % after bioremediation. The highest toxicity was detected in the first period of bioremediation; however, certain toxic effects were detectable during the whole bioremediation process. The contact tests were the...
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