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Energy sources and consumption
Majer, Jan ; Kuba, Pavel (referee) ; Bobák, Petr (advisor)
The thesis describes basic methods of producing energy and presents the basic division and characterize sources of energy. It compares and describes the production of energy in the Czech Republic and the United States of America. The thesis explains, where and how is this energy consumed and presents energy production and consumption in the Czech republic and in the United States of Amerika and provides a comparison of the two countries. Indicates the percentage of consumption in various sectors in these countries and the distribution of energy consumed by resource used. The thesis is also focused on the comparison of household consumption in the Czech Republic and the United States of America. It specifies the average household consumption of Prague and New York and investigates the causes of the difference in average household consumption of both countries.
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Calculation of steam pipeline
Majer, Jan ; Dohnal, Miloslav (referee) ; Nekvasil, Richard (advisor)
This thesis describes issues related to design and structural analysis of steam pipeline. Analysis are done by using two different software program – AutoPIPE and Ansys. Issues related to pipeline support system and thermal expansion are solved by using AutoPIPE. Structural analysis results are evaluated in accordance with ČSN EN 13 480 code.
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Preparation and characterization of diamond-based nanocarriers for transfection of siRNA
Majer, Jan ; Cígler, Petr (advisor) ; Fišer, Radovan (referee)
Although nanodiamonds were discovered and produced tens of years ago, they have been utilized in medical and biological fields just recently, particularly in drug and gene delivery into a cell and in bioimaging methods. Nanodiamonds can be modified with specific positively charged moieties for complexation with negatively charged nucleic acids. These complexes afterwards overcome extracellular and intracellular barriers and transport the nucleic acid either into cytosol or into the nucleus. Owing to fluorescence centres nitrogen- vacancy, which can be formed in the nanodiamonds, nanodiamonds exhibit excelling optical properties, as they emit stable fluorescence without "photoblinking" or "photobleaching". This thesis reviews properties, synthesis and modifications of nanodiamonds and other selected nanoparticles and their in vitro applications. This thesis also compares their cytotoxicity and gene knockdown efficiency.
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Dynamics of modified diamond nanocrystals in living cells
Majer, Jan ; Libusová, Lenka (advisor) ; Fišer, Radovan (referee)
Nanodiamonds (NDs) are an interesting platform in biological applications and disease treatment. Because of their photoluminescence properties and modifiable surface, they have been investigated as potential carriers for drugs and nucleic acids as well as fluorescent probes. In order to design NDs meeting specifically desired parameters, which would succeed in clinical trials and in medicinal therapy, understanding the mechanism of uptake and intracellular fate of NDs is crucial. The diploma thesis is focused on mechanistic investigation of ND-based nanoparticles delivering nucleic acids to human cells. First, NDs coated with a novel cationic co-polymer were prepared. NDs were then complexed with siRNA in order to transfect siRNA inside U-2 OS cells. NDs proved to be biocompatible and effective transfection particles as observed by qPCR and colorimetric cytotoxicity and cell viability tests. To examine ND uptake by cells, we inhibited endocytosis by specific inhibitors. Obtained results implicated that ND uptake was clathrin- and caveolin dependent. Nonetheless, more than half of NDs was internalized by cells in a different fashion. Some NDs colocalized with early endosomes, lysosomes and caveolin-derived endosomes after internalization. Other NDs resided either in unknown cell structures or escaped from...
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Dynamics of modified diamond nanocrystals in living cells
Majer, Jan ; Libusová, Lenka (advisor) ; Fišer, Radovan (referee)
Nanodiamonds (NDs) are an interesting platform in biological applications and disease treatment. Because of their photoluminescence properties and modifiable surface, they have been investigated as potential carriers for drugs and nucleic acids as well as fluorescent probes. In order to design NDs meeting specifically desired parameters, which would succeed in clinical trials and in medicinal therapy, understanding the mechanism of uptake and intracellular fate of NDs is crucial. The diploma thesis is focused on mechanistic investigation of ND-based nanoparticles delivering nucleic acids to human cells. First, NDs coated with a novel cationic co-polymer were prepared. NDs were then complexed with siRNA in order to transfect siRNA inside U-2 OS cells. NDs proved to be biocompatible and effective transfection particles as observed by qPCR and colorimetric cytotoxicity and cell viability tests. To examine ND uptake by cells, we inhibited endocytosis by specific inhibitors. Obtained results implicated that ND uptake was clathrin- and caveolin dependent. Nonetheless, more than half of NDs was internalized by cells in a different fashion. Some NDs colocalized with early endosomes, lysosomes and caveolin-derived endosomes after internalization. Other NDs resided either in unknown cell structures or escaped from...
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Preparation and characterization of diamond-based nanocarriers for transfection of siRNA
Majer, Jan ; Cígler, Petr (advisor) ; Fišer, Radovan (referee)
Although nanodiamonds were discovered and produced tens of years ago, they have been utilized in medical and biological fields just recently, particularly in drug and gene delivery into a cell and in bioimaging methods. Nanodiamonds can be modified with specific positively charged moieties for complexation with negatively charged nucleic acids. These complexes afterwards overcome extracellular and intracellular barriers and transport the nucleic acid either into cytosol or into the nucleus. Owing to fluorescence centres nitrogen- vacancy, which can be formed in the nanodiamonds, nanodiamonds exhibit excelling optical properties, as they emit stable fluorescence without "photoblinking" or "photobleaching". This thesis reviews properties, synthesis and modifications of nanodiamonds and other selected nanoparticles and their in vitro applications. This thesis also compares their cytotoxicity and gene knockdown efficiency.
|
|
|
Calculation of steam pipeline
Majer, Jan ; Dohnal, Miloslav (referee) ; Nekvasil, Richard (advisor)
This thesis describes issues related to design and structural analysis of steam pipeline. Analysis are done by using two different software program – AutoPIPE and Ansys. Issues related to pipeline support system and thermal expansion are solved by using AutoPIPE. Structural analysis results are evaluated in accordance with ČSN EN 13 480 code.
|
|
|
Energy sources and consumption
Majer, Jan ; Kuba, Pavel (referee) ; Bobák, Petr (advisor)
The thesis describes basic methods of producing energy and presents the basic division and characterize sources of energy. It compares and describes the production of energy in the Czech Republic and the United States of America. The thesis explains, where and how is this energy consumed and presents energy production and consumption in the Czech republic and in the United States of Amerika and provides a comparison of the two countries. Indicates the percentage of consumption in various sectors in these countries and the distribution of energy consumed by resource used. The thesis is also focused on the comparison of household consumption in the Czech Republic and the United States of America. It specifies the average household consumption of Prague and New York and investigates the causes of the difference in average household consumption of both countries.
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