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Miniaturizovaný řasový biotest
Lukavský, Jaromír
Growth algal bioassay is based on long-term cultivation of a testing alga with tested substance in nutrient solution, or in a sample from nature. The bioassay is at least 3 generations and also chronic affects or influence of repruction process can be so detected. The yield of biomass of testing alga in a sample from nature represent the maximal biomass which could be expected in nature without limitation with light, temperature, CO2 and predation.Miniaturized bioassay is exploating cultivation of algae in wells of an immunological plate in volumes of 0.2 ml. In a plate 9x13 cm, there are 96 wells which enables us to expose 10 samples every in 6 replicates, 2x25 plates in a cultivation unit of size of a common table has capacity of 3 000 miniaturized cultures. Their filling, inoculation and evaluation can be facilitated with a great spectrum of tools and equipments for immunological assays.
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