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Role of the parliamentary party groups in the constitutional system of the Czech Republic
Červinka, Lukáš Lev ; Antoš, Marek (referee)
- 145 - Abstract Role of the Parliamentary Party Groups in the Constitutional system of the Czech Republic The ultimate goal of my work was to fill in the blank spaces on the map of the Constitutional system of the Czech Republic by thorough examination and detailed description of the role and the status of the parliamentary party groups (PPGs) in it. However, this thesis is not just a general introduction, but a complex study of the actors of such importance and influence that it keeps one wondering, why they had remained out of a scope of the mainstream scientific research in our country for so long. After the necessary theoretical introduction to the subject of the parliamentary party groups within the field of the legal and political sciences, I examined the existence of the PPGs within the Chamber of Deputies, their relationships, interdependence and interconnections with the political parties and finally their inner functioning in close detail. It was necessary to deal with a number of serious issues and answer several important questions during my research, most notably: the question of the nature of the parliamentary party groups, i.e. whether they are bodies of the Chamber of Deputies, bodies of the political parties or whether they even possess their own legal personality. It was necessary to deal...
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Microfltration nanofibrous membrane for wastewater treatment
Lev, J. ; Holba, Marek ; Došek, M. ; Kalhotka, L. ; Detvanová, L. ; Dostálová, L. ; Přichystalová, J. ; Vincent, I. ; Kimmer, D.
Pilot testing of nanofiber membrane module demonstrated long-term stability of the filtration process in activated sludge and showed the applicability in real-world conditions at the wastewater treatment plant. Microbial contamination of samples of the filtrate reached log removal 2 CFU/mL. Filtration in combination with UV irradiation resulted in a reduction of microbial contamination under required legislative standards. These results supported our assumptions to use nanofibre membranes in membrane bioreactor. Regeneration, optimization and antifouling surface treatment will be another step to increase the competitiveness of nanofiber membranes for water treatment.
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Bacteria deactivation and removal from Wastewater and polluted Air
Kimmer, D. ; Vincent, I. ; Dudák, J. ; Bergerová, E. ; Petras, D. ; Lev, J. ; Holba, Marek ; Kalhotka, L. ; Mikula, Přemysl ; Kořínková, R. ; Kubáč, L.
Procedures permitting to prepare homogeneous functionalized nanofibre structures based on polyurethanes modified by phthalocyanines (PCs) by employing a suitable combination of variables during the electrospinning process are presented. Compared are filtration and bacteria deactivation properties of nanostructures without PCs, modified in bulk and with PCs embedded into polyurethane chain by a covalent bond protecting the release of active organic compound during the filtration process. Finding that the functionalized nanofibre structures have an effect on bacterial growth was confirmed by microbiological, physico-chemical and molecular biological analyses, such as the inoculation in a nutrient agar culture medium, flow cytometry and real-time polymerase chain reaction.
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Experimental Study on Bacteria Removal from Artificial and Real Wastewater by Nanofibrous Filters
Lev, J. ; Holba, Marek ; Kalhotka, L. ; Mikula, Přemysl ; Kimmer, D.
Unique properties of electrospun nanofibrous materials could be utilized in various environmental technologies, e.g. bacteria removal from water. Recent studies show various removal efficiencies of bacteria and particulated matter by nanofibrous filters. Our article presents results from filtration tests through polyurethane nanofibrous material on short-term scale with artificial wastewater and also results from tests with real wastewater. We determined E. coli abundances in artificial and real wastewaters and the amount of the other hygienically important bacteria in real wastewater samples only. The results show possibility of long-term full-scale application of those materials, however some material modifications are needed to be accomplished.
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Can Nanofibers Replace Current Microfiltration Materials?
Lev, J. ; Holba, Marek ; Došek, M. ; Kalhotka, L. ; Kimmer, D.
The electrospun nanofibrous material M247 can be used as antimicrobial filter. Laboratory tests show high removal value (up to 6 log10 CFU/mL) and similar filtration efficiency compared filter Pragopor 6, presently used for sterilization of liquids in laboratories. Semi-pilot test confirmed good mechanical endurance and high removal efficiency. Our study showed high removal value with non-functionalized nanofibrous material despite of other cited studies. These results confirm our expectations, that nanofibrous material can replace the current filtration materials for laboratory use.
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Using of flow cytometry for the detection of bacterial filtration efficiency in (waste)water treatment processes
Mikula, Přemysl ; Lev, J. ; Kalhotka, L. ; Holba, Marek ; Kimmer, D. ; Maršálek, Blahoslav ; Vítězová, M.
Aim of the study was to detect filtration efficiency of several (nano)materials used for the filtration/removal of E. coli bacteria or natural bacterial communities from water samples. Filtration efficiency was evaluated by commonly used culticvation techniques as well as by flow cytometry (FCM). Although filtration efficiency of certain nanomaterials detected by cultivation was very high (>99%), it was demonstarted that sample filtration does not have to lead to significant decline of total bacterial counts measured by FCM. From this viewpoint FCM can be considered as a suitable analytical method for the assessment of wastewater treatment processess efficiency, which, compared to cultivation techniques, possess some disadvantages (difficult standardization) but also has several advantages (speed, more realistic measurement conditions).
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