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National Repository of Grey Literature

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Methodology of resistance evaluation of plum, Prunus domesica L., clone C5 to the Plum pox virus and mixed infections with another two viruses Polák, Jaroslav ; Kumar, Jiban ; Jarošová, Jana The methodology encloses the procedure of preparation and ínoculation of transgenic plants of plum with the individual viruses, the maintenance of field orchard, the evaluation of virus symptoms in leaves, serological and electronmicroscopical detection of viruses in leaves of plum trees, determination of relative concentration of PPV by DAS-ELISA, detection of PPV-Rec, ACLSV and PDV by RT-PCR, and determination of risk of recombinations between PPV-Rec and the homologous transgenic transcript in P. domestica, clone C5. Fulltext: PDF Detailed record

Methods of detection of wheat and barley strain of Wheat dwarf virus in vector Psammotettix alienus by PCR-RFLP Jaňourová, Blanka ; Ripl, Jan ; Kumar, Jiban Wheat dwarf (causal agent Wheat Dwarf Virus - WDV) is one of the most important cereal diseases in the Czech republic where it can cause huge economic losses in wheat, barley, triticale, rye as well as in oat. Psammotettix alienus is the only known vector of WDV while another way of transmission is unrecognized. This methodology describes a PCR assay for sensitive detection of Wheat Dwarf Virus in Psammotettix alienus, and RFLP assay for barley and wheat strains specific identification. Fulltext: PDF Detailed record

National Repository of Grey Literature : 12 records found   11 - 12  jump to record:

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