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Antiphospholipid antibodies and their avidity in selected groups of diseases
Kuchař, Oliver ; Fialová, Lenka (advisor) ; Štípek, Stanislav (referee) ; Navrátil, Leoš (referee)
The heterogeneity of antiphospholipid antibodies (APA) may be reflected not only in the specificity of different antigens but also in their avidity. APA avidity appears to be clinically useful as a valuable additional characteristic. APAs that are not included in the laboratory criteria for antiphospholipid syndrome (APS), the, so-called non-criteria antibodies, have been detected in patients with seronegative antiphospholipid syndrome. Antiphosphatidylethanolamine (aPE) antibodies represent one type of APA directed against neutral phospholipids, phosphatidylethanolamines. The main objectives of this dissertation were: 1) to compare several modifications of ELISA (enzyme-linked immuno sorbent assay) with different chaotropic reagents for the determination of avidity of anticardiolipin antibodies (ACLA), 2) to investigate the relationship between ACLA IgG avidity and their levels, 3) to evaluate the levels and avidity of aPE as an example of non-criteria antibodies in several groups of patients and compare them with conventional APAs. To compare different methods for determining the avidity of ACLA IgG, we tested a modified ELISA method using different concentrations of urea and sodium chloride as chaotropic reagents and different serum dilutions. We analyzed the levels and avidity of ACLA and aPE...
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