|
|
Analysis of essentiality of glmM gene coding for phosphoglucosamine mutase of Streptococcus pneumoniae.
Krupička, Jiří ; Branny, Pavel (advisor) ; Čáp, Michal (referee)
Phosphoglucosamine mutase (GlmM) is an enzyme of bacterial cell wall biosynthesis. The main aim of this thesis was to find out, whether gene glmM is essential for viability of Streptococcus pneumoniae. Therefore, we prepared merodiploid strain containing two copies of glmM; the genomic gene and ectopic copy under control of zinc inducible promoter. Subsequently, depletion strain was prepared by deletion of genomic copy of glmM. This strain was further used for analysis of viability and phenotype features in the medium containing various concentrations of zinc ions, an inducer of ectopic glmM expression. We found out, that the viability of this strain was strictly dependent on the concentration of inducer and further, that depletion of GlmM resulted in remarkable morphological defects. The rescue of mutant strain was observed after addition of inducer up to the level of the control sample. These results have provided the evidence of glmM essentiality for S. pneumoniae viability. Furthermore, we analyzed, whether phosphorylation of key amino acid residues, S99 and S101, is essential for GlmM functionality. Four different strains were prepared by means of site-directed mutagenesis expressing glmM with substitutions of key serine residues for alanine or glutamic acid. Since deletion of chromosomal locus in...
|
|
|
Forensic ballistics
Papírníková, Lenka ; Konrád, Zdeněk (advisor) ; Krupička, Jiří (referee)
The aim of this thesis is to introduce the basic information about the forensic ballistics and about the procedure of ballistic experts. The first chapter is dedicated to clarification the fundamental terms of the subject matter of this thesis. Beside the term of forensic ballistics I also introduce the meaning of some other related terms used in the following text. Next chapter deals with the history and development of the ballistics. The key feature of every ballistic researches are the ballistics marks described in the third chapter. I focus on the ballistic marks division, the origin and occurrence and receiving of these. The gunshot residues are mentioned in their own subchapter. The objects of the ballistic researches, listed in the chapter four, are firearms, ammunition and animate or inanimate target. Next chapter deals with the range of ballistic researches which can be divided into two groups. Within the frame of the identification examination of firearms and ammunition, the individual and group identification of the gun used in a criminal offence is performed. That means the ballistic marks from the seized firearm are compared with the ones detected in a crime scene. Within the frame of the non- identification examination the firearms and ammunition characteristics, the shooting direction,...
|
|
|
Methods of investigation of murders (selected issues)
Saňáková, Ivana ; Musil, Jan (advisor) ; Krupička, Jiří (referee)
The thesis "Methodology of Investigation of Murders (selected issues)" deals only with the selected aspects of the methodology of this crime. Since the topic is too broad, it is not within the author's possibilities to cover the topic completely while working on this thesis. For this reason, only selected points of view on the investigation of this serious crime are discussed in details. The thesis is divided into two main chapters. The first chapter is devoted to the introduction to this topic. The initial subchapter provides a wider view of crime murder from the perspective of criminal law. It also describes the legal regulation providing the basic human right, specifically the right to life, as stated in the Czech legal order, including the documents at European or international level as well. The second subchapter deals with the physical as well as fault elements of this crime. The whole chapter is interwoven with historical aspects and background, while the author also comes with the comparison of current legislation with the previous one. The following subchapter contains the comparison of this crime with the similar crimes, particularly manslaughter, infanticide, negligent homicide and also the participation in suicide. In the next section, current statistics about the quantity of murders in...
|
|
|
Fingerprinting - Past, Present and Future
Semera, Lukáš ; Krupička, Jiří (advisor) ; Konrád, Zdeněk (referee)
FINGERPRINTING - PAST, PRESENT AND FUTURE The objective of this thesis is to present the forensic fingerprinting as a scientific method which, although in comparison with other forensic sciences being one of the oldest, is not out-dated at all, quite to the contrary - it presents an ever-changing and continuously developing field of science. The objective is approached through three distinctive and yet intertwined chapters corresponding with the title of the thesis - past, present and future of the fingerprinting. They are also designed, at least in part, to capture this constant forward motion. Accordingly, the first chapter aims to describe briefly scientific roots of the method, the way it drew in its beginnings from various scientific discoveries, inspired by the rise of Darwinism. Particular emphasis is put on the description of the formulation and empirical confirmation of three physiological laws of fingerprinting that even today serve as its grounds. The second chapter deals with and tries to describe the current state of knowledge and particular methods used in detection, development and preservation of fingerprints, especially latent ones. To do this it seems to be necessary to describe briefly the anatomy and physiology of friction ridge skin, as it enables to fully comprehend the way in...
|
|
|
Issues of punishment for traffic offences
Klinovský, Karel ; Říha, Jiří (advisor) ; Krupička, Jiří (referee)
My thesis is focused on the issues of the punishment for traffic offences. This topic is actually in the spotlight of the social discourse since the relatively high number of tragic traffic accidents leads to the call for the solution of the anomy on the Czech roads. One of possible solutions is also a proportional use of the state repression using the legal tools of the criminal law. The goal of this thesis is an analysis of all the punishments applicable to the traffic offences and their de lege lata and de lege ferenda evaluation. In the first part of this thesis, I will define all kinds of transportation and their legal basis. This is necessary to put the road traffic offences into their proper place in the system of traffic related offences. Furthermore, I define the traffic offence and I also elaborate on which offences are traffic offences per se. My conclusion is that we should use more restrictive approach when it comes to listing the traffic offence. Later on, I modify this restrictive approach by adding the offences of reckless endangerment and obstruction of an official decision. As a part of this chapter, I also examine each offence in the context of their practical use and judicial decisions. The second part of this thesis is focused on the traffic offender. A very specific aspect of...
|
|
|
Dactylography - History, Present and Future
Jelínek, Milan ; Konrád, Zdeněk (advisor) ; Krupička, Jiří (referee)
Dactylography - History, Present and Future This thesis introduces one of the most important identification methods - dactylography (or dactyloscopy), e.g. the method based on analysis and classification of patterns of friction ridges (especially fingerprints). This thesis consists of three main chapters considering its name. The first chapter describes interesting history of this method from first discoveries, through various breakthroughs, to reaching the status of forensic evidence. This chapter mainly focuses on individual pioneers on the field of dactylography, but tells as well the story of Czechoslovak dactylography and reminds cases, that were very close to prove this method wrong at its fragile beginning. The second chapter focuses on the present of dactylography. This chapter starts with three basic laws of dactylography, but the essence of it lies in the term "dactyloscopic evidence". The methods of revelation, analysis and preservation of dactyloscopic evidence are described here as well as their comparison. If it comes to analysis, I am trying to compare two approaches used today (holistic and numerical) and offer my opinion which one is better. Also, I am trying to answer the question, if it is possible to tell how old the dactyloscopic evidence is. The third and the last chapter...
|
|
|
Identification by a line-up
Hanušová, Zuzana ; Konrád, Zdeněk (advisor) ; Krupička, Jiří (referee)
The topic of my thesis is a recognition in criminal proceedings. The thesis deals with recognition in terms of crimilonogy and criminal procedure. The recognition is one of the important evidence, which are often based on evidence in criminal proceedings. At first sight it may seem that a recognition is easy procedure in criminal proceedings, but closer examination reveals its complexity. The aim of the thesis is to introduce the recognition as a special way of proving and its specifics, summarization of the knowledge gained by studying textbooks and monographs, articles in journals and some judicial decisions, as well as outlining the selected questions and poiting out possible problems, especially the obligation of the defendant to attend the recognition. The thesis is divided into eight chapters, which are divided to subchapters. Following the introduction, the first chapter defines the concept of recognition, and then focuses on psychological basics of recognition, historical evolution of recognition as an evidence since 1873 and also its grounding in the Code of Criminal Procedure. Second chapter describes the relationship between recognition and other criminal methods, poiting out some of the common features and also its differences. Third chapter defines different types of recognition - with...
|
|
|
Methods of investigation of murders (selected issues)
Polívková, Lucie ; Štourač, Petr (advisor) ; Krupička, Jiří (referee)
in English The topic of the thesis is the " The Methodology of Investigation of Murders (selected issues)". The topic is too broad for the limited capacity of the dissertation. While writing the thesis, after the necessary theoretical introduction (which contains the definition of crimes against life, medical definition of death, etc.), I focused only on the initial stage of the investigation of the crime of murder. I specifically focused on the discovery of the body, on securing the crime scene, on autopsy and on the demarcation of investigative versions. Next stage of the investigation is only briefly summarized in the ninth chapter. The thesis is divided into ten chapters. The first chapter explains basic terms, especially murder, murder of a newborn baby by its mother and other crimes against life, such as manslaughter, negligent homicide and assistance to suicide. This chapter also includes medical definition of death, and a brief description of the post-mortem changes. The second chapter consists of brief statistics on the number of murders in the Czech Republic between the periods 2000 to 2012. It contains two graphs; the first graph shows the evolution of the total number of murders each year and their solving. The second graph divides murders according to the specific motives. The third...
|
|
|
Crime of murder and klling under Section 140 and 141 of the Czech Criminal Code
Rossmannová, Kateřina ; Hořák, Jaromír (advisor) ; Krupička, Jiří (referee)
CRIME OF MURDER AND CRIME OF MANSLAUGHTER ACCORDING TO § 140 TZ, § 141 TZ The topic of my thesis is the crime of murder (§ 140) and the crime of manslaughter (§ 141 of the Criminal Code). These are the most serious crimes directed against human life. As the topic of human life is very important for my work, the first chapter is focused on the description of the legal regulation of the protection of human life in the terms of Czech and international regulation as well as on definition of the beginning and the end of human life. In this chapter I have also included the infanticide. In the next chapter I dealt with the constituent elements of the crime of murder and manslaughter. Separately, I spoke about the object, the physical element, the subject and the mental element of these crimes. In the section discussing the object I focused on the subject of attack and on the victim, too. Under the discussion about the subject I tried to define the subject of the crime of murder. The fourth chapter is devoted to the classification of crimes of murder and manslaughter under the valid Criminal Code and to comparison of new and old conception of these crimes arising from the new and the old Criminal Codes valid in the Czech republic. The following chapter is focused on the crime of murder. In this chapter I...
|
|
|
Non-conventional bacterial signaling pathways
Krupička, Jiří ; Branny, Pavel (advisor) ; Beranová, Jana (referee)
Two component systems were traditionally considered as main phosphorylation systems of bacteria involved in cell signalling. Recently, attention focuses increasingly on bacterial eukaryote-like Ser/Thr protein kinases (eSTKs). These protein kinases are structurally similar to their eukaryotic counterparts. Some eSTKs possess additional domains such as extracellular PASTA domains that were discovered in a variety of gram-positive bacteria. It has been proved that these domains can act as sensors for unlinked peptidoglycan fragments. However, majority of environmental signal molecules still remains unknown. eSTKs phosphorylate a broad spectrum of substrates including proteins involved in various cell processes such as virulence, cell wall biosynthesis, cell division, and central and secondary metabolism. Cross talk between eSTKs and two component systems also occurs. In this thesis, the current knowledge about eSTKs and their significant substrates in different bacterial species is discussed.
|
guest ::
RSS feed.