|
|
Determination of the expression of enzymes DHRS7B and DHR7C in human tissues
Kozáková, Klára ; Wsól, Vladimír (advisor) ; Štambergová, Hana (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Klára Kozáková Supervisor: prof. Ing. Vladimír Wsól, Ph.D. Title of diploma thesis: Determination of the expression of enzymes DHRS7B and DHRS7C in human tissues Dehydrogenase/reductase (SDR family) member 7B (DHRS7B) and 7C (DHRS7C) are human enzymes that belong to the short chain dehydrogenase/reductase (SDR) superfamily. This very old and extensive superfamily includes members, which play an important role in different physiological and pathological processes. In spite of that the SDR enzymes are currently more studied, still about half of these enzymes are only poorly characterized, such as DHRS7B and DHRS7C. The aim of this study was the determination of the expression profiles of human enzymes DHRS7B a DHRS7C at mRNA and protein level and thus brings new knowledge of these enzymes. Expression of enzymes was tested in human tissues. RNA was isolated from the samples and used for transcription to cDNA. Obtained cDNA was utilized as a template for qPCR with fluorescence SYBR GREEN I detection. Determination of enzymes at protein level was done in homogenates of human tissues by using a Western blotting with chemiluminescence detection. The level of expression mRNA DHRS7B was strongest in testes...
|
|
| |
|
|
Valuation of the company Arysta LifeScience
Kozáková, Klára ; Špička, Jindřich (advisor) ; Strouhal, Jiří (referee)
The aim of this master thesis is to assess the objectified value of equity of the company Arysta LifeScience Czech s.r.o. Estimation is conducted from the parent company's perspective, assessing the value of equity before the moment of purchase of the parent company by American firm Platform Specialty Products. Discounted cash flow method based on FCFE is used for the valuation. Essential foundation for application of this method is the financial plan, which is based on internal company data and performed strategic and financial analysis. Monte Carlo simulation is applied to the DCF method in order to verify the accuracy of determining the value of equity under uncertainty. Moreover, sensitivity analysis is used to identify risk factors, which are impacting value of equity the most. Discounted cash flow method is complemented by asset-based approach, specifically by the book value method. The value of equity based on income approach is estimated to be 932 367 thousand CZK.
|
|
|
Financial analysis of the company Arysta LifeScience Czech s.r.o.
Kozáková, Klára ; Špička, Jindřich (advisor) ; Krause, Josef (referee)
The aim of this bachelor thesis is to assess financial health of the company Arysta LifeScience Czech s.r.o. during the period of 2006 - 2011. Theoretical part of this thesis contains the calculation methodology of the indicators of financial analysis, including justification for their use. The practical part of this thesis focuses on assessment of financial health of the company using the indicators introduced in the theoretical part. Financial analysis is conducted from the perspective of managers, using internal company data. The conclusion summarizes the findings of the financial situation, identifies strengths and weaknesses of the company and offers recommendations for improvement of the current situation.
|
guest ::
RSS feed.