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Control circuits of railway lift
Kopecký, Jan ; Lažek, Tomáš (referee) ; Huták, Petr (advisor)
This master thesis deals with the mechanical and electrical design of the drive of the inclined construction lift. Individual components are selected in the design. Furthermore, a theoretical research is carried out regarding wireless modules and logic controllers. The selection of sensors is made and the method of retrieving signals from sensors and converting them into iputs the control circuits is also outlined. A program is written in Arduino IDE that wirelessly controls the motion of a motor via variable frequency drive.
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determination of kinetic parameter of reactions using DSC
Kopecký, Jan ; Kouřil, Miloslav (referee) ; Jan, Vít (advisor)
The master thesis deals with DSC measurments a calculation of kinetic parameters. In the theoretical part, iron alotropy is briefly described, followed by a description of the DSC analysis itself. The thesis also includes division and derivation of isoconversional methods for calculating the activation energy. In the experimental part, a series of measurments is perfomed on pure iron prepared by Cold Spray method. In the paper the influence of deformation on the Curie temperature of the magnetic transformation in -Fe and transformation > is investigated. The activation energy is calculated for transformation > for different degrees of deformation. The degree of deformation is monitored by channeling contrast.
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Design of synchronous spoke motor
Kopecký, Jan ; Bulín, Tomáš (referee) ; Knebl, Ladislav (advisor)
This thesis is focused mainly on the theoretical research of permanent magnet synchronous motors. In addition, various methods of designing the motor‘s magnetic circuit are discussed and their advantages and disadvantages are compared. The reluctance network method is analyzed more thorough, because this method is used for motor designing process. In the last part the results obtained by reluctance network are compared with the results evaluated by finite element method.
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Environmental drivers of microbial community composition in a temperate forest
Martinović, Tijana ; Baldrian, Petr (advisor) ; Vašutová, Martina (referee) ; Kopecký, Jan (referee)
Recently, much attention has been focused on the importance of forests to the global carbon cycle. Microorganisms are a critical component of the carbon cycle in forests, participating in important ecosystem processes and degrading various carbon compounds, from simple to highly recalcitrant compounds. However, most studies in this regard have focused on fungi. Only in recent years has it become known that bacteria are also capable of degrading complex plant polymers in soil, and that their contribution to the carbon cycle may be as important as that of fungi. Furthermore, our knowledge of the temporal dynamics of both fungal and bacterial communities is limited, as well as their response to different environmental drivers. The main objective of this dissertation was to fill these knowledge gaps by (1) quantifying the rate of temporal turnover of fungal and bacterial communities in soils of temperate forests, (2) describing the factors that shape fungal communities in spruce and beech stands during their long-term development (stand age), (3) assessing the contribution of fungi and bacteria to the utilisation of different carbon sources in forest soils, and finally (4) describing the development of bacterial communities in response to clearcutting of a spruce stand. The study of microbes...
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Microbial communities of soils affected by long-term heavy metal contamination
Průchová, Pavla ; Kopecký, Jan (advisor) ; Matyska Lišková, Petra (referee)
This work is focused on microbial communities living in the soil affected by long-term exposure to heavy metals. The soil was sampled at two sites with different levels of contamination near Příbram. In the samples, respiration rate was measured in vitro after addition of carbon sources and at different levels of cadmium, one of the contaminating metals found in the soil. After the incubation with carbon sources, soil samples were collected for environmental DNA isolation. Gene coding for 16S rRNA in Actinobacteria was amplified from the environmental DNA samples and the amplicon composition was assessed by terminal restriction fragment length polymorphism analysis. The resulting profiles were used to compare actinobacterial communities in both groups of soil samples and in individual treatments. The analysis showed a clear distinction between the two sites differing in the contamination level and shifts in the community composition of various intesity depending on the added substrate.
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Effect of ectopic synthesis of mitochondrial uncoupling protein 1 in white adipose tissue on whole-body metabolism in mice
Janovská, Petra ; Kopecký, Jan (advisor) ; Drahota, Zdeněk (referee) ; Kalous, Martin (referee)
The prevention and treatment of obesity is a major problem of health care systems in affluent societies. Metabolism of adipose tissue belongs to the therapeutical targets, since accumulation of adipose tissue is the basis of obesity development. Experiments using transgenic mice with ectopic expression of brown- fat uncoupling protein 1 (UCP1) in white adipose tissue (WAT), verified a concept that obesity could be ameliorated by increasing energy expenditure in WAT. The goal of the experiments of this PhD Thesis was to characterize in detail the phenotype of this unique animal model of obesity resistance. We have shown that mitochondrial uncoupling in WAT resulted in increased oxidation of fatty acids (FA), in face of decreased lipogenesis and induced mitochondrial biogenesis in this tissue. In further studies, we aimed to modulate propensity to obesity be increasing FA oxidation in WAT in response to physiological stimuli. This could be accomplished in response to the combination treatment using n-3 polyunsaturated fatty acids (n-3 PUFA) and mild calorie restriction in mice fed high-fat diet. Synergistic induction of mitochondrial oxidative capacity and lipid catabolism in epididymal WAT was associated with suppression of low-grade inflammation of WAT, which is typical for obesity. The improvement of lipid...
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Soil microbial communities in agroecosystems and natural habitats contributing to resistance and resilience of the soil environment
Sarikhani, Ensyeh ; Kopecký, Jan (advisor) ; Demnerová, Kateřina (referee) ; Větrovský, Tomáš (referee)
Ensyeh Sarikhani Soil microbial communities in agroecosystems and natural habitats contributing to resistance and resilience of the soil environment. Summary The control of common scab of potatoes (CS) includes resistant varieties (cultivars), precise fertilization, increase of soil moisture, and chemical treatments. Yet, these management practices do not have common or reproducible results at differing sites. A monitoring study was done in 32 sites to evaluate the relation between CS and biological/chemical soil parameters. Correlations were observed between scab severity and content of nutrients such as Fe, N, and Ca in soil and periderm, and between disease severity and abundance of actinobacteria and total bacteria, together with the pathogenicity determinant, txtB gene (biosynthetic gene of thaxtomin) in both soil and periderm of potatoes. The findings led to novel conclusions, which can help to understand relationships applicable in scab control. Peat and DTPA chelated iron were supplemented to pots filled with soil conducive for CS in order to determine the effects of soil organic matter, iron and pH on CS development. The results were compared with data obtained for a suppressive soil from a nearby field with naturally low CS severity. Both peat and iron supplements decreased CS and the combination...
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