|
|
Evaluation of zolpidem tartrate using HPLC
Kopecká, Iva ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
Charles University in Praque, Faculty of Pharmacy in Hradci Králové Department: Pharmaceutical Chemistry and Drug Control Candidate: Iva Kopecká Tutor: PharmDr. Pavla Pilařová, Ph.D. Name of Degree Paper: Evaluation of zolpidem tartrate using HPLC In the diploma thesis, there was optimazed the method of separation of zolpidem tartrate and its impurity A. There was tested the influence of the composition of the mobile phase, molarity of phosphate buffer, its pH and the temperature on the column. The optimal conditions of the separation were determined - methanol:acetonitril: 0,06 mol/l phosphate buffer pH 4 (20:15:65), the temperature on the column 30řC, the flow rate of the mobile phase was 1ml/min and the detection in the UV in the wavelength of 254 nm. Afterwards, the method was used for rating of stability tests in these load conditions - alkaline environment, increased temperature, oxidative environment and daylight. The decrease of the concentration of effective substance of zolpidem tartrate in the given environment and time was observed as well as the potential development of the destructive products of the effective substance.
|
|
|
Chromatographic evaluation of amiodarone and its active metabolite
Kopecká, Iva ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
77 Abstract Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department: Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Mgr. Iva Kopecká Tutor: PharmDr. Pavla Pilařová, Ph.D. Name of Degree Paper: Chromatographic evaluation of amiodaronu and its active metabolite The main purpose of this thesis was to optimize the existing HPLC method used in University Hospital Hradec Králové for determination of amiodarone and its active metabolite in biological material. For this HPLC method was used Waters Symetry C18 column, 150 x 4,6 mm, 5 µm, mobile phase: acetonitrile: 25 mM phosphate buffer (55:45), flow rate 1,4 ml/min, at 45 ř C, injection 5 µl, UV detection at 242 nm. This method was subsequently transferred to a UHPLC using Phenomenex Kinetex C18 column, 100 x 2,1 mm, 1,7 µm, mobile phase: acetonitrile: 25 mM phosphate buffer (55:45), flow rate 0,3 ml/min, at 45 ř C, injection 5 µl, UV detection at 242 nm. This UHPLC method was modified for eventual detection by mass detector using same UHPLC column, mobile phase: acetonitrile: 0,1% formic acid (55:45), flow rate 0,3 ml/min, at 45 ř C, injection 5 µl, UV detection at 242 nm. At the same time plasma, resp. serum sample isolation was optimized. The best result was achieved by adding 4 µl of 10% zinc sulfate to the plasma sample...
|
|
|
Chromatographic evaluation of amiodarone and its active metabolite
Kopecká, Iva ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
77 Abstract Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department: Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Mgr. Iva Kopecká Tutor: PharmDr. Pavla Pilařová, Ph.D. Name of Degree Paper: Chromatographic evaluation of amiodaronu and its active metabolite The main purpose of this thesis was to optimize the existing HPLC method used in University Hospital Hradec Králové for determination of amiodarone and its active metabolite in biological material. For this HPLC method was used Waters Symetry C18 column, 150 x 4,6 mm, 5 µm, mobile phase: acetonitrile: 25 mM phosphate buffer (55:45), flow rate 1,4 ml/min, at 45 ř C, injection 5 µl, UV detection at 242 nm. This method was subsequently transferred to a UHPLC using Phenomenex Kinetex C18 column, 100 x 2,1 mm, 1,7 µm, mobile phase: acetonitrile: 25 mM phosphate buffer (55:45), flow rate 0,3 ml/min, at 45 ř C, injection 5 µl, UV detection at 242 nm. This UHPLC method was modified for eventual detection by mass detector using same UHPLC column, mobile phase: acetonitrile: 0,1% formic acid (55:45), flow rate 0,3 ml/min, at 45 ř C, injection 5 µl, UV detection at 242 nm. At the same time plasma, resp. serum sample isolation was optimized. The best result was achieved by adding 4 µl of 10% zinc sulfate to the plasma sample...
|
|
|
Evaluation of zolpidem tartrate using HPLC
Kopecká, Iva ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
Charles University in Praque, Faculty of Pharmacy in Hradci Králové Department: Pharmaceutical Chemistry and Drug Control Candidate: Iva Kopecká Tutor: PharmDr. Pavla Pilařová, Ph.D. Name of Degree Paper: Evaluation of zolpidem tartrate using HPLC In the diploma thesis, there was optimazed the method of separation of zolpidem tartrate and its impurity A. There was tested the influence of the composition of the mobile phase, molarity of phosphate buffer, its pH and the temperature on the column. The optimal conditions of the separation were determined - methanol:acetonitril: 0,06 mol/l phosphate buffer pH 4 (20:15:65), the temperature on the column 30řC, the flow rate of the mobile phase was 1ml/min and the detection in the UV in the wavelength of 254 nm. Afterwards, the method was used for rating of stability tests in these load conditions - alkaline environment, increased temperature, oxidative environment and daylight. The decrease of the concentration of effective substance of zolpidem tartrate in the given environment and time was observed as well as the potential development of the destructive products of the effective substance.
|
|
|
Quality Management in Education
Kopecká, Iva ; Trunda, Jiří (advisor) ; Trojan, Václav (referee)
The main aim of my bachelor thesis is a perception of a quality management by headmistresses of kindergarden. Their opinions and arguments about the quality management of a school and their clarifications. The bachelor thesis is suppose to discover what awareness do the headmistresses of the kindergarden have about opportunities of the quality management. What are their opinions about methods of the quality management and their introducing to a school system. What form of introducing would bring benefits to competitive environment of the kindergardens. The bachelor thesis should bring real description, opinions and stance of the kindergarden's headmistresses on a education system, its quality management, their potential arguments and their reasons. Keywords: Quality, quality management, quality in education, quality of pre-school education, methods of quality management.
|
|
|
Analýza bankovního produktu se zaměřením na studentské konto
Kopecká, Iva ; Malý, Václav (advisor) ; Samek, Petr (referee)
V magisterské práci byl proveden marketingový výzkum studentských kont nabízených bankami na českém trhu. Byly analyzovány faktory, podle kterých se studenti rozhodují při výběru konta. Na základě této analýzy a analýzy konkurence bylo navrženo konto Independent. Konto je založeno na svobodném výběru jednotlivých prvků studentského konta, které jsou uspořádány do pyramidy. V každém patře pyramidy konta si student podle předem stanoveného schématu volí balíčky služeb. Výsledkem je, že si klient může navrhnout své konto podle svých představ a neplatí za služby, které nepotřebuje.
|
guest ::
RSS feed.