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National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Amplitude and phase objects observation through scattering media by means of coherence-controlled holographic microscope
 Effenberger, Adam ; Lošťák, Martin (referee) ; Kollárová, Věra (advisor)
This diploma thesis deals with phase and amplitude objects observation through scattering media by means of a coherence-controlled holographic microscope (CCHM). A brief history of development and construction of the microscope, its advantages compared to the classical light microscopy and hologram processing are described. Quantitative phase imaging through scattering media by means of ballistic as well as diffuse light is verificated in the experimental part. A comparison of an image obtained through a scattering layer by means of CCHM and a classical microscopy in the light field is demonstrated.
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Emission difference in superresolution fluorescence microscopy
 Havelka, Tomáš ; Kollárová, Věra (referee) ; Bouchal, Petr (advisor)
Fluorescence microscopy finds broad applications in biological sciences as well as in technical research. Due to the complete spatial incoherence of the radiation emitted from the sample, fluorescence microscopy serves as the foundation for a range of super-resolution techniques. The bachelor's thesis is focused on the method of emission difference. This method utilizes the difference between standard and vortex image to achieve super-resolution. In this work, a q-plate is used to create the vortex image. The q-plate is a liquid crystal device that replaces costly spatial light modulators used in previous implementations of the method. The method of emission difference is described in the bachelor's thesis through theoretical calculations and numerical modeling at both the point and two-dimensional imaging levels. The experimental part of the work involves the preparation of an optical setup for the practical testing of the investigated method. The main result of the thesis is the original implementation of the method of emission difference using a q-plate and the verification of its capability to achieve super-resolution in imaging fluorescence nanoparticles.
Detailed record
Amplitude and phase objects observation through scattering media by means of coherence-controlled holographic microscope
 Effenberger, Adam ; Lošťák, Martin (referee) ; Kollárová, Věra (advisor)
This diploma thesis deals with phase and amplitude objects observation through scattering media by means of a coherence-controlled holographic microscope (CCHM). A brief history of development and construction of the microscope, its advantages compared to the classical light microscopy and hologram processing are described. Quantitative phase imaging through scattering media by means of ballistic as well as diffuse light is verificated in the experimental part. A comparison of an image obtained through a scattering layer by means of CCHM and a classical microscopy in the light field is demonstrated.
Detailed record

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4 Kollárová, Veronika
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