|
|
Genus Haemophilus and laboratory examinations in medicine today
KADLECOVÁ, Jana
My bachelor´s thesis focuses on the most major pathogen of the genus named Haemophilus, and Haemophila influenzae. H. influenzae is an originator of more serious infections, mainly its type b, which is the most virulent organism, causing invasive diseases mainly with children and older people. It causes mainly meningitis and chronic obstructive pulmonary disease. In the theoretical part of my bachelor´s thesis I write about the genus Haemophilus and clinic significances of the most famous species which occur at present. The genus Haemophilus is an aerobic and a facultative aerobic gram that means it is a negative bacteria, which is characteristic for its arduous requests for accretion. I used some methods for the diagnosis. I used the following methods a test with growth factors, which is a common diagnostic method, which distinguishing Haemophilus according to their addiction to the growth factors. This method is financially very demanding and currently the mason being why its results are not so accurate. But thanks to a new technology a more modern method called MALDI TOF weight spektronomie (MS) was found, which is easier, quicker and more accurate than the first one. MALDI TOF enables a diagnosis of the most demanding pieces of bacteria, yeast and mushrooms. The MALDI TOF diagnosis is characterized by undisputed reliability and cost effectiveness. Lactam antibiotics, mainly antibiotics - co-amoxicilin and chloramfenikol are used as treatment methods against Heamophilus. Against serious infections can one protect themselves with a vaccination the Hib vaccination. In the practical part of my bachelor´s thesis I described some processes and laboratory diagnostics of the pathogen H. influenza by methods MALDI TOF MS and by the Test for growth factories in a microbiological laboratory named Synlab, czech s.r.o., in České Budějovice. The results of how frequently H. influenza occurred in the year 2013, were gained from of the previously mentioned laboratory information system and were transported into the other laboratory from a field office of general practisioners. The other aim of my bachelor´s thesis was to compare severity and of each method. HYPOTHESIS 1: The frequency of H. influenzae in the year 2013 depends on a season. HYPOTHESIS 2: The MALDI TOF MS method is reliable and fast for identification of the genus Haemophilus. The Test with growth factories is not so reliable and fast. From the results it is obvious that the frequency of H. influenzae depends on the season. Heamophilus are very demanding and sensitive bacteria for cultivation and they need optimal conditions for their accretion. In this season the viral diseases increase and they can affect the process of the treatment. During the comparison of these two methods I managed to prove that the second hypothesis MALDI TOF is useful in a diagnosis. This method is not demanding. And the result can be obtained in a few minutes. At the end of my thesis it can be said that the MALDI TOF MS method is fast, reliable and an efficient diagnosis tool for identification of microorganisms. MALDI TOF MS has become an effective method for taxonomic classifications and microbiological studies. The MALDI TOF MS method is fast and there are minimal costs for consumables in samples.
|
|
|
The seniors´activation in the Our Lady´s Home of peaceful old age of the Regional charity in Klatovy
KADLECOVÁ, Jana
This bachelor's thesis deals with the activation of seniors. The theoretical part describes an aging, old age and its effects on the seniors´ activities. It characterizes the forms and kinds of activization programmes, by which we can act against these impacts. It provides the basic conditions for the success of activization programmes. The practical part characterizes the residential facilities of social services, the Home for the Elderly - The Our Lady´s Home of peaceful old age of the Regional charity in Klatovy, and the using of activization programmes there.
|
|
| |
|
|
Characterization and function of the transferrin from the tick \kur{Ixodes ricinus}
KADLECOVÁ, Jana
Iron metabolism is an important physiological process in ticks which has a great potential for an efficient tick control. This thesis is focused on the characterization and function assessment of the transferrin-related protein in the hard tick Ixodes ricinus. The full sequence Ixodes ricinus transferrin was determined and revealed that this protein is related to the insect transferrins of the type 2 (melanotransferrins). RNA interference silencing of tick transferrin did not affect tick feeding and survival suggesting that function of this protein is not directly involved in the iron transport.
|
|
| |
|
| |
|
|
Tests of thermal and radiation embrittlement of reactor steel 15CH2MFA
Dvořáková, M. ; Kadlecová, Jana ; Konop, R. ; Tomáš, Ivan ; Vértesy, G.
Results are presented of mechanical destructive and magnetic nondestructive tests of the 15Ch2MFA steel, used for pressure vessels of the VVER40 reactor type. Two series of samples were tested: (i) samples, embrittled by a special thermal processing, and (ii)samples, embrittled in a reactor by high fluences of neutron radiation. Results of mechanical impact Charpy tests are compared with results of Magnetic Adaptive Testing carried out on the two sample series. The existence of mutual correlation between the two types of examination indicates alternative of replacement of the mechanical destructive tests of classical surveillance samples by magnetic tests, which are nondestructive.
|
|
|
VOJTA PRINCIPLE IN THE PHYSIOTHERAPY OF LOCOMOTIVE DISORDERS BY THE CHILDREN´S NEUROLOGICAL PATIENTS
KADLECOVÁ, Jana
My bachelor thesis deals with reflexology in relation to treatment of children with cerebral palsy (CP). As the Vojta method has been considered the most effective for treatment of patients suffering from any form of cerebral palsy, I have chosen as the main objective of my thesis the evaluation of effectiveness of treatment with the Vojta method. The respondents were two clients of the Arpida center for rehabilitation of handicapped people, who were undergoing treatment for triparetic and diparetic form of cerebral palsy. In the theoretical part I described facts about the disorder, including its etiology, symptoms, clinical manifestations but particularly diagnostics, prevention and treatment. Subsequently, I described the essence of the Vojta method as a diagnostic and therapeutic system. The practical part used a method of qualitative research, based on a secondary analysis of data and direct observation. The monitoring included a standard observation test Gross Motor Function Classification System (GMFCS) and ADL test. To meet one partial objective of the thesis, i.e. analysis of experience of parents with treatment of CP using the Vojta method, I used a method of semi-structured interview. The examination of spontaneous mobility and the quality and quantity of body posture in the development of erective mechanisms has confirmed improvement in both the respondents after the applied therapy using the Vojta method, which has been demonstrated in the final kinesiology analysis. The case studies have shown that effectiveness of the therapy using the Vojta method is influenced by certain aspects, such as frequency, dosing, early beginning of the therapy, proper management of parents by the physiotherapist and the parents´ attitude to the therapy. The Vojta method represents a good basis for treatment of children with cerebral palsy but the rehabilitation shall be always an integral process. In conclusion of my thesis I have recommended to monitor clients in their development by using retardation quotient calculations.
|
|
|
QUESTIONS OF SAFETY IN THE INSTITUTIONS INTENDED FOR WATER HOLIDAY ACTIVITIES
KADLECOVÁ, Jana
``Water-related injuries{\crqq} are the second most frequent cause of death of children in the Czech Republic. My thesis addresses the issue of safety in facilities designed for aquatic leisure activities. Within the scope of the qualitative research with the aim to analyze technical standards, to ascertain their observance and afterwards to analyze the injury risks I used the data collection technique consisting in secondary data analysis, observation in combination with photographic documentation and semi-standardized interviews. For the research I selected a swimming pool in České Budějovice and Aquaviva Wellness Centre in Aurora Třeboň spa. The reason for this selection was creation of a pilot study monitoring safety in these facilities in South Bohemia. The Czech Republic has technical standards in place that are applied to minimize the risk of injury to the largest possible extent in facilities like that. They determine the procedures that have to be observed during construction and subsequent operation of facilities designed for aquatic leisure activities. The provisions defined by the standards are observed in both facilities but in their substance they are so generalized that it is only at the operators{\crq} discretion to what extent the safety is to be secured by them. They should be more specified and applied concretely to individual units of the equipment in order to create a safe environment. As far as risk places in the facilities are concerned, it may be stated that, regardless of compliance with the requirements defined by the standard, there is a large number of risks related in particular with slippery surfaces. Within the scope of the safety evaluation, it is also necessary to take account of hazardous behaviour of users of the chutes. The results of the research indicate that in Aquaviva Wellness Centre in Třeboň safety is secured and complies with the requirements of technical standards and the prevention requirements; the swimming pool in České Budějovice shows more serious defects {--} the standards are complied with but the surfaces are not covered with materials that would secure safety. On the basis of my research and other technical studies that confirmed seriousness of water-related injuries it is obvious that the issue of injuries in this field will require modification of safety measures and more precise specification of standards and thus elements such as materials of surfaces that would secure the required safety.
|
|
|
Analysis of selected genomic regions with dominant suppressors of the \kur{adgf-a} mutant phenotype in \kur{Drosophila melanogaster}
KADLECOVÁ, Jana
Adenosine is an important signaling molecule in various physiological processes in many different organisms. Dysregulation of the level of this molecule leads to many pathological consequences as Severe Combined Immunodeficiency in humans. Recently a genetic model using fruit fly Drosophila melanogaster was established to study effects of adenosine in this organism and a genetic screen for genes modifying the effects of heightened adenosine was started. This work presents partial tasks in this screen which include detailed characterization of selected regions which were identified as dominant suppressors of the phenotype associated with heightened adenosine. First task was to produce a smaller, molecularly defined deletion in range of originally identified bigger deletion. Second task included a testing of already existing smaller deletion for the suppressing effects. In the third task, a role of potential suppressor was further analyzed which included a verification of P-element insertions in this gene and their testing for suppressing effects. In the last task, the sequence for making a probe to characterize the expression of this gene was cloned
|
guest ::
RSS feed.