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Texture Analysis of Ophthalmologic Images
Kaňka, Jan ; Odstrčilík, Jan (referee) ; Jan, Jiří (advisor)
This thesis is concerned with creating of software for textural analysis of retinal images by statistic method called co-occurrence matrices as possible alternative method to detection of lesion of retina by the glaucoma diseases. The Glaucoma diseases without well-timed diagnostics and consequential treatment leads to blindness. The retinal images captured by fundus camera are common, easily processable and modern devices are able to make images of 10 Mpix resolution and bigger, what specifies outputs of analytic software. Method of co-occurrence analysis is simple and effective statistic. But computing demandingness, showing up with growing objective quality, comming up as disadvantage.
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Controlling and Evaluation of Laser Micromanipulation Experiments
Kaňka, Jan ; Jákl, Petr (referee) ; Provazník, Ivo (advisor)
This work is focused on the development of a user friendly software interface using the LabViewTM environment that simplifies running of various experiments using laser micromanipulations and laser microspectroscopy of living microorganisms. Both techniques have been developing very fast for the last decade and belong to the growing group of contact-less and nondestructive techniques for manipulation and diagnostics of individual living microorganisms, cells, or viruses. Within this project we mastered the driving of peripheries, calibration of CCD scene, real-time image processing of the CCD scene, automatic selection of the cell for further laser processing, acquisition and processing of the Raman spectrum from living microorganisms. The final goal of our activity is fully automatic laser-based sorter of living cells depending on their chemical compositions. This work has been elaborated at the Institute of Scientific Instruments of the ASCR, v.v.i. under the supervision of prof. Pavel Zemanek.
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Texture Analysis of Ophthalmologic Images
Kaňka, Jan ; Odstrčilík, Jan (referee) ; Jan, Jiří (advisor)
This thesis is concerned with creating of software for textural analysis of retinal images by statistic method called co-occurrence matrices as possible alternative method to detection of lesion of retina by the glaucoma diseases. The Glaucoma diseases without well-timed diagnostics and consequential treatment leads to blindness. The retinal images captured by fundus camera are common, easily processable and modern devices are able to make images of 10 Mpix resolution and bigger, what specifies outputs of analytic software. Method of co-occurrence analysis is simple and effective statistic. But computing demandingness, showing up with growing objective quality, comming up as disadvantage.
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Controlling and Evaluation of Laser Micromanipulation Experiments
Kaňka, Jan ; Jákl, Petr (referee) ; Provazník, Ivo (advisor)
This work is focused on the development of a user friendly software interface using the LabViewTM environment that simplifies running of various experiments using laser micromanipulations and laser microspectroscopy of living microorganisms. Both techniques have been developing very fast for the last decade and belong to the growing group of contact-less and nondestructive techniques for manipulation and diagnostics of individual living microorganisms, cells, or viruses. Within this project we mastered the driving of peripheries, calibration of CCD scene, real-time image processing of the CCD scene, automatic selection of the cell for further laser processing, acquisition and processing of the Raman spectrum from living microorganisms. The final goal of our activity is fully automatic laser-based sorter of living cells depending on their chemical compositions. This work has been elaborated at the Institute of Scientific Instruments of the ASCR, v.v.i. under the supervision of prof. Pavel Zemanek.
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SMV-2015-36: „Light Sheet“ microscope combining three wavelengths
Ježek, Jan ; Kaňka, Jan ; Jákl, Petr ; Šerý, Mojmír
The equipment is used for 3D scanning of fluorescence in biological samples. Three lasers with wavelengths suitable for biological samples are used for the excitation of fluorescent dyes. The image is scanned by a highly sensitive CCD camera. Switching between different excitation lasers is controlled by a computer. The observed sample is held in a holder mounted on a four-axis table (3 axes for moving a sample, one axis of rotation around the axis of the sample). The equipment is used to capture images of the sample in different positions of the positioning table.
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From the mere pushing to sorting of microparticles and assembly of microrobots by light
Zemánek, Pavel ; Arzola, Alejandro V. ; Brzobohatý, Oto ; Chvátal, Lukáš ; Jákl, Petr ; Kaňka, Jan ; Karásek, Vítězslav ; Šerý, Mojmír ; Šiler, Martin
We demonstrate the recent progress in the field of optical micromanipulation. We start with the classical applications of solar sail propelled by the radiation pressure and approach the recent ones dealing with optical tractor beams, optical binding and sorting of microobjects. The pioneering attempts to assemble a microrobot by light is presented.
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Specific forms of users interaction with holographic optical tweezers controlled by leap motion
Keša, P. ; Tomori, Z. ; Kaňka, Jan ; Jákl, Petr ; Šerý, Mojmír ; Bernatová, Silvie ; Antalík, M. ; Zemánek, Pavel
Optical tweezers allows contactless three-dimensional manipulation with electrically neutral objects of sizes from tens of nanometers to tens of micrometers. Mouse or joystick are the traditional devices to control the positions of trapping beams in holographic optical tweezers that provide optical manipulation with microobjects. Our approach is based on a modern 3D depth sensors combined with gestures and speech recognition software. The human operator controls the position of one trapping beam by a traditional pointing device like mouse or joystick. However, manipulation with more objects needs control of corresponding number of independent trapping beams generated by a spatial light modulator. We upgraded own NUI software which processes signals from sensors and sends the control commands to holographic Raman tweezers which subsequently controls the positions of trapping microparticles and the acquisition system of Raman spectra.
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