National Repository of Grey Literature 57 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
Rodinný dům s prodejnou
Kučera, Lukáš
The thesis theme is the design of a family home with a shop in the ground floor, located on the land of the current building for demolition. The property is designed for a family of four working from home. This is a non-basement house, with the shop on the ground floor being a walled and residential part of a wooden frame structure. The work is divided into text and drawing sections with attachments. The text section contains basic explanations of the used frame structure of the woodwork, the issue of barrier-free use of structures needed for the proposed house, criteria of low-energy buildings, options with dealing with flat roofs and fire safety of buildings. The practical part involves creating a documentation of the demolition works to obtain a permit for the removal of the structure and then creating the design documentation of the new building using two structural systems. The work also includes thermal technical assessment of perimeter structures and assessment of the building's fire safety.
Study of de novo purine synthesis in model systems of Caenorhabditis elegans and HeLa cell lines
Součková, Olga ; Zikánová, Marie (advisor) ; Šebesta, Ivan (referee) ; Kučera, Lukáš (referee)
Purines are involved in many biologic processes and are required to maintain stable energy charge, for metabolic regulation, as cofactors in enzymatic reactions and as building blocks in DNA and RNA. Necessary role in purine synthesis is played by de novo purine synthesis (DNPS) that is highly active in developing cells. DNPS is complex pathway involving enzymes that assemble into multienzyme complex, purinosome, which facilitates flux of purine intermediates through sequence of ten enzymatically catalyzed reactions. Properly functioning enzymes and purinosome ensure the fast flux of unstable and potentially cytotoxic intermediates resulting in final product IMP, the branch point for AMP and GMP synthesis. The mutations in DNPS genes lead to inherited rare disorders that are accompanied by elevated concentrations of enzyme substrates in body fluids and cells. Lack of a suitable model system to study pathophysiology of DNPS raised the necessity to develop a system mimicking DNPS disorders. HeLa cells with malfunctioning DNPS were characterized on the level of genes, transcripts, proteins, metabolites, and for the presence of the purinosome. Homozygous or compound heterozygous mutations led to the absence of proteins, decreased enzymatic activities, accumulation of enzyme substrates, downregulation...
Vegetation effects on ambient air quality alongside the motorways
Kučera, Lukáš ; Hůnová, Iva (advisor) ; Lhotka, Radek (referee)
The bachelor thesis is a literature search on the removal of pollutants emitted by traffic with the use of vegetation barriers along public roads. The aim of the thesis is to investigate the reasons leading to contradictions existing in the literature about the effectiveness of vegetation barriers. It explores which type and structure of vegetation barrier is the most effective in removing pollutants from the air. On the basis of the conducted research and available data, it determines whether it makes sense to consider introduction of vegetation barriers along H6 Úlibice-Hořice and H7 Hořice-Sadová, the segments of the D35 highway. The first part of the paper outlines that the effectiveness of a vegetation barrier is influenced by a number of factors such as the wind speed and its direction, precipitation summary, vegetation density,itś height and species composition or dimensions of vegetation barriers itself. For this reason, there is no general type or structure of vegetation barrier that improves air quality, and it is therefore essential to desingn such barrier on a site-specific basis. The reason for negative impact of some vegetation barriers on air quality was due to their improper design. The second part of the thesis deals with the suitability of planting vegetation barriers on selected...
Family house
Kučera, Lukáš ; Šoulová, Eva (referee) ; Vlček, Milan (advisor)
The subject of this bachelor's thesis is the design of project documentation for the construction of a family house in Brno. The construction plot is located in the Jundrov district in the northwestern part of the city of Brno. The house is designed as four floors, of which one floor is underground. There is one apartment unit in each above-ground floor and two parking spaces per unit. The project documentation also includes an assessment concerning building physics and the concept of technical equipment installations. The work was processed using the following programs: Microsoft Word, Microsoft Excel, Microsoft PowerPoint, Adobe Acrobat Pro, Autodesk AutoCAD 2022, BuildingDesign, SketchUp, Lumion.
THE ANALYSIS OF MARKETING STRATEGY OF A HOTEL
KUČERA, Lukáš
The thesis is related to the sector of services in the hotel industry. One of partial aims of this pthesis was a thorough analysis of the historical advancement till the present days. The marketing and management methods of SWOT analysis, STEP analysis and a questionnaire have been used to elaborate the work. In addition, various marketing strategies which could be put to use for the hotel in question have been evaluated. However, the main target of the work was a draft of such a marketing strategy that will appear the most advanced in the future.
Accurate pedometers function using the autocalibration
Kučera, Lukáš ; Slabý, Kryštof (advisor) ; Procházka, Michal (referee)
v angličtině Aim: The aim of this study is to explore the possibilities of accurate pedometer functions through a partial removal of individual measurement errors for a particular pair of pedometer- proband, using the auto-calibration of walking for the previously known distance. Methods: They will be used pedometers of mechanical type YAMAX SW-200 and fully electronic type OMRON HJ-720. Thirty adults from 18 to 69 years old (11 men, 19 women) took part in this study. In the pilot phase will be suggesting of a sufficiently long sections of known length (100 m, 300 m a 1000 m) and auto-calibration on a flat track. Own attempt will consist in landscaping measurement. Resulsts: From everage lengths and from the number of steps, which we measured, is possible to determine distance, which probands covered. The biggest aproach was to real distance 1000 m in case we used everage length of step from distance 300 m. In this case undervaluation of the distance reached in Omron 7 % and with Yamax just 10 %. Howewer the distance as well for Omron was overvalued in 27 % probands and for Yamax it was in 20 % probands. Conclusion: The best possibility seems to be average length of step, which is couted from distance for 100 m. Although there is undervaluation more frequent, overvalution is minimal. It will be...
Preparation of affinity surfaces for mass spectrometry
Kalaninová, Zuzana ; Pompach, Petr (advisor) ; Kučera, Lukáš (referee)
One of the most potent toxic substances of biological origin known are botulinum toxins - nerve toxins produced by strains of Clostridium bacteria. They affect neuro-muscular connections where they cleave proteins of the SNARE complex, block neurotransmitter release, and thus stop signal transmission. This leads to flaccid paralysis, the main symptom of a disease called botulism. Due to their extremely low lethal dose and high toxicity, BoNTs take a place on the list of agents of bioterrorism. Despite numerous methods available to this date, the mouse bioassay remains the gold standard for the detection of BoNTs. Regardless of the ethical aspects of these experiments, the time they require is also inconvenient. In this work, a unique combination of protein surfaces and detection of active BoNTs through their endopeptidase activity and mass spectrometry, EndoPep-MS, is introduced. Functionalized surfaces were prepared by ambient ion soft landing - a technique utilizing a beam of hyperthermal ions (energy range 1-100 eV) as a tool for controlled modification. Two approaches to the detection of botulinum toxin A1 were applied. One of them, based on avidin- biotin interaction, used a neutravidin-modified surface for enhancement of biotinylated peptides (substrate peptide for BoNT/A and peptide products...
Design of Supplier-Customer Relationships in Terms of FinancialTies
Kučera, Lukáš ; Sedláčková, Magda Piknová (referee) ; Jurová, Marie (advisor)
The bachelor’s thesis deals with an evaluation of the situation of individual entities from a selected company on the basis of information and documentation provided by the company. The thesis consists of theoretical part and practical part, of which theoretical part consists of individual definitions which are related to the thesis, it deals in more detail with claims which then helps realization of the analysis in the practical part. The practical part focuses on the analysis of supplier-customer relationships within individual entities of the selected company. The invoices issued in 2020 within the entities provided by the company were subjected to the analysis. The results of the analysis were then evaluated, commented and on the basis of the results are written recommendations for improvements and innovations in the company.
Residue analysis from archaeological ceramics using specific antibodies and mass spectrometry
Pavelka, Jaroslav ; Kučera, Lukáš (konzultant)
Important information preserved in the form of biological markers - so-called organic residues containing food residues - can be preserved on ceramics under special conditions. By analyzing them, it is possible to determine the type of food being prepared or consumed in the past. For this reason, a laboratory procedure based on the ELISA method (Enzyme-Linked Immuno Sorbent Assay) was developed, primarily aimed at determining the presence of denatured proteins from medieval ceramics, which uses available and certified kits for immunological analysis. The approved methodology is intended for scientific experts from the ranks of archaeologists, bioarchaeologists and immunologists working with archaeological material.
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National Repository of Grey Literature : 57 records found   1 - 10nextend  jump to record:
See also: similar author names
7 Kučera, Ladislav
1 Kučera, Leoš
5 Kučera, Libor
4 Kučera, Luboš
42 Kučera, Lukáš
42 Kučera, Lukáš
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