|
|
Použití kapalinové chromatografie v analýze vzorků životního prostředí
KŘÍŽOVÁ, Monika
This work deals with the use of HPLC method in the analysis of environmental samples. Cigarette butts are one of the most common garbage in nature. These cigarette butts may contain high amounts of polycyclic aromatic hydrocarbons (PAHs) and many other toxic and chemical compounds, which may contaminate the environment. One of the aims of this work was to optimize extraction process of 16 priority PAHs from freshly smoked cigarette butts and the HPLC/FLD analytical method and consequently to determine their contents in collected samples. The second part of the work is devoted to the introduction of a method suitable for the quantitative evaluation of metformin loss in 100% aqueous samples. The method will be further used for the analysis of standard solutions in the monitoring and evaluation of possible photochemical reactions of metformin.
|
|
|
Circadian changes of the cholinergic system in various areas of the central nervous system
Křížová, Monika ; Mysliveček, Jaromír (advisor) ; Jakubík, Jan (referee)
Muscarinic acetylcholine receptors type 4 are one of the five subtypes of metabotropic muscarinic receptors whose knockout led to disruption of the biorhythm represented by locomotion in female mice. In this thesis, we attempted to determine the biorhythmic activity of selected components of the cholinergic system in structures of the central nervous system which are suspected to regulate circadian locomotion. These selected structures were the striatum, motor cortex, thalamus, intergeniculate leaflet, subparaventricular zone and posterior hypothalamic area. Changes of muscarinic receptor density in wild type and M4 receptor knockout female mice were measured by using autoradiography throughout the day. In the striatum, motor cortex, and thalamus, the density of muscarinic receptors type 1 and the activity of acetyl- and butyrylcholinesterases were also measured by autoradiography and the Ellman's method. Rhythmic oscillations of muscarinic receptor density were observed in all structures selected in both types of mice. The main brain structure responsible for the differences in the biological rhythm of wild type and M4 receptor knockout mice was identified as the striatum and motor cortex region, where the percentage of M4 receptors was highest. Diurnal oscillations were also exhibited by...
|
|
|
Role of M4 muscarinic receptors in the central nervous system
Křížová, Monika ; Mysliveček, Jaromír (advisor) ; Slepička, Jakub (referee)
Muscarinic receptors type 4 are one of the five subtypes of muscarinic receptors, that are members of cholinergic receptors family, together with nicotinic receptors. An acetylcholine binding on the receptor triggers the receptor activation and the signal transmission into the cell by G-proteins located on plasmatic membrane, in the case of muscarinic receptors type 4 by Gi/o proteins. The M4 receptors are, just like other subtypes on muscarinic receptors, located in central and peripheral nervous system. In the central nervous system, they are mostly located in striatum and hippocampus. The M4 receptors have a whole range of regulative functions, the most significant one is most likely the regulation of a locomotion in striatum by the dopaminergic system. The muscarinic receptors type 4 are involved in many behavioural and cognitive processes and are therefore studied as potential drug target for the treatment of mental disorders. Key words: muscarinic receptors, M4 muscarinic receptors, GPCRs, cholinergic system, central nervous system
|
|
|
Role of M4 muscarinic receptors in the central nervous system
Křížová, Monika ; Mysliveček, Jaromír (advisor) ; Slepička, Jakub (referee)
Muscarinic receptors type 4 are one of the five subtypes of muscarinic receptors, that are members of cholinergic receptors family, together with nicotinic receptors. An acetylcholine binding on the receptor triggers the receptor activation and the signal transmission into the cell by G-proteins located on plasmatic membrane, in the case of muscarinic receptors type 4 by Gi/o proteins. The M4 receptors are, just like other subtypes on muscarinic receptors, located in central and peripheral nervous system. In the central nervous system, they are mostly located in striatum and hippocampus. The M4 receptors have a whole range of regulative functions, the most significant one is most likely the regulation of a locomotion in striatum by the dopaminergic system. The muscarinic receptors type 4 are involved in many behavioural and cognitive processes and are therefore studied as potential drug target for the treatment of mental disorders. Key words: muscarinic receptors, M4 muscarinic receptors, GPCRs, cholinergic system, central nervous system
|
|
|
Antidecubital Care as a Nursing Problem
KŘÍŽOVÁ, Monika
This thesis deals with the issue of providing antidecubital care and observes the fulfilment of nursing interventions in the care about non-self sufficient patients. During performing her duties, every nurse encounters the issue of decubitus ulcers formation and with this related antidecubital care. Thus, it can be stated that the occurrence of decubitous ulcers serves also as the indicator of the quality of provided nursing care. A lot of people think that the problem develops particularly in elders, but young people who are bedridden after injuries and accidents are also affected and this complication degrades the quality of their life. The thesis is divided into theoretical and empirical parts. The theoretical part describes the anatomy of skin and its function, decubitus and classification of decubitus ulcers are defined, their localization with characteristics of predilection sites are specified, outlining complications in decubitus ulcers. The theoretical part pursues antidecubital care with the division into subheads on positioning, antidecubital aids, nursing interventions, and the mission of the European Pressure Ulcer Advisory Panel is described. The separate chapter includes the risk assessment of decubitus ulcers formations, as is e.g. Norton scale, etc. The last chapter embraces the specification of conservative and surgical treatment of pressure sores. The aim of the research examination was to determine whether the nurses perceived antidecubital care as a nursing problem and to map the opinions of the nurses relating to antidecubital care. Three research questions were set to achieve the objectives: first, what the pitfalls of nursing care were on the part of the nurses, secondly, what areas within antidecubital care were neglected and finally, how antidecubital care at selected inpatient wards was provided. The research was implemented at the chosen inpatient wards in České Budějovice Hospital, PLC, from the period of February to April 2014. The research file consisted of the general nurses and the medical assistants. A hidden participant observation was conducted and then questionnaires with 15 questions that were closed or semi-closed were distributed to the nurses. 45 questionnaires were handed out, 31 handed back and all 31 questionnaires could be processed. Thus, the total return of the questionnaires was 69%. Questions in the questionnaire were based on the observation criteria. The hidden participant observation was processed and plotted on the observation sheet and then classified according to the given criteria. After delivery, the returned questionnaires were checked and evaluated graphically. All results were processed using Misrocoft Office Excel program, were plotted in graphs with keys and descriptions. By processing and comparing the results, we ascertained that in some fields the opinion of the nurses was different from the practice that was really performed. It could be also caused by the fact that antidecubital care is very time-consuming, demanding and comprises a lot of nursing interventions. Sufficient number of aids with which the ward is equipped is relevant as well, be it preventive aids or medical ones. And last but not least the organization of the care. At present, the time spent on completing documentation is increasing and the time that can be used by nurses for contacting a patient and satisfy his/her needs is decreasing. Nevertheless, the patient´s state and his/her contentment reflect and demonstrate our care. After previous arrangement, the results of our research examination will be communicated to the ward sisters at the selected inpatient wards where the research examination was performed.
|
|
|
Asylum support as a social life net.
KŘÍŽOVÁ, Monika
Cílem práce je podat co nejširší přehled o způsobech a metodách práce v azylovém domě (zaměřeno na azylový dům pro matku a dítě), seznámit s vnitřními pravidly, se službami, které jsou uživatelkám sociální služby k dispozici, popsat sociální vrstvy potencionálních klientek a jejich dětí, obeznámit s nejčastějšími důvody pobytu v azylovém zařízení.
|
guest ::
RSS feed.