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HPLC method development and validation for analysis of anthocyanins in highbush blueberries
Křížová, Kateřina ; Šatínský, Dalibor (advisor) ; Sklenářová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Kateřina Dostálová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of Diploma Thesis: HPLC method development and validation for analysis of anthocyanins in highbush blueberries HPLC method for simultaneous determination of anthocyanins: delphinidin-3-O-galactoside, delphinidin-3-O-glucoside, cyanidin-3-O-galactoside, cyanidin-3-O-glucoside, cyanidin-3-O- arabinoside, peonidin-3-O-glucoside and malvidin-3-O-galactoside in 21 varieties of blueberries ('Spartan', 'Sunrise', 'Toro', 'Bluegold', 'Croatan', 'Northland', 'Duke', 'Gila', 'Jersey', 'Bluecrop', 'Herbert', 'Berkeley', 'Patriot', 'Darrow', 'Bluetta', 'Collins', 'Brigitta', 'November', 'Iranka', 'Bluejay' and 'Rancocas') was developed and optimized in this thesis. Kinetex PFP column, 150 x 4.6 mm (particle size 2.6 µm) was used for the analysis. The detection was performed by DAD detector at 520 nm, column temperature of 50řC using gradient elution with a mobile phase of acetonitrile/ 2 % formic acid at a flow rate of 1.0 ml/min. The total time of the analysis of one sample took 21 minutes. Keywords: HPLC, blueberries, anthocyanins, polyphenols, antioxidants, delphinidin-3-O- galactoside, delphinidin-3-O-glucoside,...
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Multifunctional house
Šenfeld, Pavel ; Šilerová-Křížová, Kateřina (referee) ; Brukner, Bohuslav (advisor)
The aim of the thesis is to project a family house with a workroom and to work out a plan. The house is being built on building site no. 1127/28 in the town of Hoštka. The project is developed for four to six residents, with the workroom (separated side entrance). Self-contained house ground plan is in the form of the letter L (47.57 × 50.85 ft.). Disposition of the projected single storey house is 5+1 with the habitable attic. There are entry foyer, hall, bathroom, toilet, living room, utility room, garage, workroom with kitchenette, toilet and multifunctional lobby on the first floor. Hall, bedroom, two other rooms and bathroom with toilet are located in the habitable attic. The house is projected on concrete strip. As far as the supporting part of the building is concerned, the Porotherm system is used. Horizontal construction above the first
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