|
| |
|
|
Isolation and characterization of catechol 1,2-dioxygenase of Candida tropicalis
Jechová, Jana ; Stiborová, Marie (advisor) ; Martínková, Markéta (referee)
Candida tropicalis yeast is a microorganism that possesses high tolerance for phenol and strong phenol degrading activity. This yeast is capable of utilizing phenol as the sole source of carbon and energy without formation of any secondary waste product. Catechol-1,2- dioxygenase was isolated from cytosolic fraction of this yeast by the procedure consisting of chromatography on DEAE-Sepharose and gel permeation chromatography on Sephadex G- 100. The catechol-1,2-dioxygenase was purified to homogeneity. The enzyme activity was followed by HPLC (catechol consumption and/or cis,cis-muconic acid formation). The activity profiles at different temperatures showed temperature optimum of 30řC. Kinetic characterizations were studying in different values of pH. The values of Km and Vmax of 0,52 mM and 17,2 nM/min for consumption of catechol, respectively, and 0,34 mM and 12,6 nM/min for formation of cis,cis-muconic acid, respectively, were found at optimum pH of the reaction, pH 7,6.
|
|
|
Isolation and characterization of catechol 1,2-dioxygenase of Candida tropicalis
Jechová, Jana ; Stiborová, Marie (advisor) ; Martínková, Markéta (referee)
Candida tropicalis yeast is a microorganism that possesses high tolerance for phenol and strong phenol degrading activity. This yeast is capable of utilizing phenol as the sole source of carbon and energy without formation of any secondary waste product. Catechol-1,2- dioxygenase was isolated from cytosolic fraction of this yeast by the procedure consisting of chromatography on DEAE-Sepharose and gel permeation chromatography on Sephadex G- 100. The catechol-1,2-dioxygenase was purified to homogeneity. The enzyme activity was followed by HPLC (catechol consumption and/or cis,cis-muconic acid formation). The activity profiles at different temperatures showed temperature optimum of 30řC. Kinetic characterizations were studying in different values of pH. The values of Km and Vmax of 0,52 mM and 17,2 nM/min for consumption of catechol, respectively, and 0,34 mM and 12,6 nM/min for formation of cis,cis-muconic acid, respectively, were found at optimum pH of the reaction, pH 7,6.
|
|
| |
|
|
Beginning to Read and Write in the First and Second Grade
Jechová, Jana ; Wildová, Radka (advisor) ; Křivánek, Zdeněk (referee)
My diploma dissertation deals with the question whether it is possible to achieve the development of children's creativity by their early reading and writing in common school condidions. I also use various methods and forms which conduce to the development of one's creative abilities and skills. I tired to outline the creative activitis which I had pursued with pupils in the course of two schools years. I did a small survey, comparing the results of the development of children's creativity on a number of other shools.
|
guest ::
RSS feed.