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National Repository of Grey Literature 2 records found  Search took 0.01 seconds. 
Development of MEPS method for UHPLC-MS/MS determination of entecavir in renal ultrafiltrate
 Janák, Jaroslav ; Vlčková, Hana (advisor) ; Chocholoušová Havlíková, Lucie (referee)
1 Abstract Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of analytical chemistry Candidate: Jaroslav Janák Supervisor: RNDr. Hana Vlčková, Ph.D. Title of Thesis: Development of MEPS-UHPLC-MS/MS methods for the determination of entecavir in kidney ultrafiltrate. The thesis deals with the development and validation of sample preparation method for determination of entecavir in kidney ultrafiltrate, using techniques MEPS (microextraction by packed sorbent). The previously developed UHPLC-MS/MS method for the determination of entecavir in rat urine has been used for the analysis. Hydrophilic interaction chromatography by column Acquity BEH Amide and isocratic elution were employed. The composition of the mobile phase was acetonitrile and 5 mM ammonium acetate pH 4.0 in the ratio 75:25 (v:v). Entecavir C2 13 N15 was used as the internal standard. Ionization of the analyte was carried out by electrospray in the positive ion mode and triple quadrupole was employed as the detector. Quantification of the analyte was realized by two SRM transitions of entecavir and internal standard method. Based on the MEPS optimization method, porous graphitic carbon suitable for the analysis of polar compounds was selected as the most suitable sorbent. The mixture of acetonitrile and water in a...
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Development of MEPS method for UHPLC-MS/MS determination of entecavir in renal ultrafiltrate
 Janák, Jaroslav ; Vlčková, Hana (advisor) ; Chocholoušová Havlíková, Lucie (referee)
1 Abstract Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of analytical chemistry Candidate: Jaroslav Janák Supervisor: RNDr. Hana Vlčková, Ph.D. Title of Thesis: Development of MEPS-UHPLC-MS/MS methods for the determination of entecavir in kidney ultrafiltrate. The thesis deals with the development and validation of sample preparation method for determination of entecavir in kidney ultrafiltrate, using techniques MEPS (microextraction by packed sorbent). The previously developed UHPLC-MS/MS method for the determination of entecavir in rat urine has been used for the analysis. Hydrophilic interaction chromatography by column Acquity BEH Amide and isocratic elution were employed. The composition of the mobile phase was acetonitrile and 5 mM ammonium acetate pH 4.0 in the ratio 75:25 (v:v). Entecavir C2 13 N15 was used as the internal standard. Ionization of the analyte was carried out by electrospray in the positive ion mode and triple quadrupole was employed as the detector. Quantification of the analyte was realized by two SRM transitions of entecavir and internal standard method. Based on the MEPS optimization method, porous graphitic carbon suitable for the analysis of polar compounds was selected as the most suitable sorbent. The mixture of acetonitrile and water in a...
Detailed record

See also: similar author names
2 Janák, Jakub
4 Janák, Jiří
6 Janák, Josef
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