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Development of a new DNA mutation detection technology for application in cancer diagnostics
Janoušková, Kristýna ; Minárik, Marek (advisor) ; Klepárník, Karel (referee)
The aim of this diploma thesis was to test a new technology for DNA mutation detection and to optimize the parameters related to the introduction of a new method, that uses this technology, into oncological diagnostic practice. The new method consists of an experimental modification of an already used method for the detection of somatic mutations in tumor DNA, which is based on heteroduplex analysis and denaturing capillary electrophoresis. The new aspect of the technology is the use of short oligonucleotide probe, which significantly simplifies the interpretation of data and also enables the simultaneous examination of multiple mutations in one reaction (multiplexes). Key words: DNA, denaturating capillary electrophoresis, heteroduplex, mutation, PCR, probe
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Comparison of methods for the determination of ambroxol in medicinal products
Janoušková, Kristýna ; Nesměrák, Karel (advisor) ; Kozlík, Petr (referee)
This bachelor thesis aimed to compare selected analytical methods for determining the content of ambroxol in medicinal products in terms of trueness and precision of the measurement and measurement time and cost. Three methods were selected for the determination: (1) UV spectrometry using the calibration method, (2) UV spectrometry based on the standard addition method, and (3) high performance liquid chromatography. Two types of syrups, tablets, and prolonged-release capsules were chosen as tested medicinal products. Although high performance liquid chromatography was the most time and money-consuming, it gave the truest results. In contrast, both UV spectrometric determinations were the least costly and time-consuming, but the calibration line method was the least true and the standard addition method was the least precise. Key words: ambroxol, UV spectrometry, high-performance liquid chromatography
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Criminal law regulation of protection against terrorism in the Czech Rpublic
Janoušková, Kristýna ; Jelínek, Jiří (advisor) ; Vanduchová, Marie (referee)
1 Criminal law regulation of protection against terrorism in the Czech Republic Abstract This diploma thesis deals with the criminal law regulation of protection against terrorism in the Czech Republic. Its aim is to characterize this criminal law regulation, including selected related topics. The thesis is divided into four chapters. The author first introduces the reader to the issue of terrorism through a short historical excursion, then focuses on various definitions of the term terrorism and describes its characteristic features, principles, types, forms and presents it in the context of organized crime and extremism. The second chapter deals with instruments of protection against terrorism. An essential term in this context is anti-terrorism or counter-terrorism, which is a set of measures and activities aimed at preventing, suppressing and eliminating the consequences of terrorist acts and implemented through so-called "anti-terrorism policy". First of all, in this chapter, the analysis of individual terrorist threats and their relevance for the Czech Republic positively answers the question of the need for instruments of protection against terrorism, accented also by a sub-chapter dealing with phenomenon of lone wolfs and foreign terrorist fighters. Finally, two basic concepts of anti-terrorism...
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Comparison of funding selected social and health facilities from European and national sources
JANOUŠKOVÁ, Kristýna
The subject of the bachelor thesis is to compare and evaluate the depletion of financial resources from European and national sources of selected social-health care centres. In the theoretical part, is described the Regional policy of the European Union together with its goals, principle and instruments and also structural policy and funds in relationship to this Regional policy. Finally is focused in explanation of system social services in the Czech Republic and resources of its financing. The practical part focuses on history, characteristics, the services offered and the funding of social-health care centres in Kdyně and in Domažlice from European and national sources. The thesis is evaluates and detects of potential differences of current funding of two social-health care centres, hereafter a finding, whether these facilities use all possibilities of the depletion of finances from the European or national sources, which are available.
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