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The development of the microregion on topographical and thematical maps
Jílková, Jana ; Pospíšil, Lubomil (referee) ; Plánka, Ladislav (advisor)
This master thesis deals development of geographical names, cartographic symbols and on thematically maps documents changes of land use in microregion Lomnicko. Basis for its elaboration were analog and digital topographical and thematically maps from various sources and different time of periods. A part of the thesis are characteristics of used maps documents and conditions of their obtaining. Development of names and cartographic symbols is documented by tables and charts.
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Influencing of molecular mass of hyaluronic acid by fermentation process
Wikarská, Monika ; Jílková, Jana (referee) ; Velebný, Vladimír (advisor)
The biological function of hyaluronic acid (HA) – the polysaccharide, which consists of repeating units of N-actylglucosamine and glucuronic acid – is extremely broad and often contradictory, depending on its molecular weight (MW). The bacterial production provides HA of high molecular weight and purity. The objective of this thesis is to control the molecular weight of produced HA by the addition of hyaluronidases during the process of fermentation, resulting in reduction of viscosity of the medium followed by increase of yield. Hyaluronic acid was prepared by batch fermentation of the microorganism Streptococcus equi subsp. zooepidemicus. During the process of fermentation, the enzyme hyaluronate 4-glycanohydrolase (BTH) at various concentrations and different times of cultivation was added to bioreactors. The HA yield, the molecular weight and the polydispersity by the SEC-MALLS were monitored, together with the residual glucose concentration and the dynamic viscosity of the production medium. The formation of low and medium molecular weight HA was achieved. The addition of 10 U (BTH) / g (HA) had no significant effect on the molecular weight and therefore the HA function, but the corresponding drop of viscosity was noteworthy for the simplification of the product purification. Potential increase in HA yield was not confirmed because of the very low concentration of residual glucose and the method of precipitating low and medium MW HA.
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Influencing of molecular mass of hyaluronic acid by fermentation process
Wikarská, Monika ; Jílková, Jana (referee) ; Velebný, Vladimír (advisor)
The biological function of hyaluronic acid (HA) – the polysaccharide, which consists of repeating units of N-actylglucosamine and glucuronic acid – is extremely broad and often contradictory, depending on its molecular weight (MW). The bacterial production provides HA of high molecular weight and purity. The objective of this thesis is to control the molecular weight of produced HA by the addition of hyaluronidases during the process of fermentation, resulting in reduction of viscosity of the medium followed by increase of yield. Hyaluronic acid was prepared by batch fermentation of the microorganism Streptococcus equi subsp. zooepidemicus. During the process of fermentation, the enzyme hyaluronate 4-glycanohydrolase (BTH) at various concentrations and different times of cultivation was added to bioreactors. The HA yield, the molecular weight and the polydispersity by the SEC-MALLS were monitored, together with the residual glucose concentration and the dynamic viscosity of the production medium. The formation of low and medium molecular weight HA was achieved. The addition of 10 U (BTH) / g (HA) had no significant effect on the molecular weight and therefore the HA function, but the corresponding drop of viscosity was noteworthy for the simplification of the product purification. Potential increase in HA yield was not confirmed because of the very low concentration of residual glucose and the method of precipitating low and medium MW HA.
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Determinants of Foreign Language Learning
Jílková, Jana ; Prokop, Jiří (advisor) ; Betáková, Lucie (referee) ; Smetáček, Vladimír (referee)
The purpose of this doctoral thesis is to analyse chosen factors involved in foreign language acquisition, compare three types of assessment strategies of foreign language knowledge and skills to look for correlations between students' outcomes, their school and out-of-school background and time factors. In this mixed research study the data collected from documents, questionnaires and semi-structured interviews were analysed. State language examination candidates and upper- secondary students who were to take the new state school-leaving examination are involved in the study. The results show statistically significant relationship between the formal assessment by the teachers, through standardised examinations and by self-evaluation and factors as age when the learners started the foreign language learning, learning materials used and teachers' background. These findings may be used to provide feedback for strategic changes in study achievements to students, teachers and examiners.
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The development of the microregion on topographical and thematical maps
Jílková, Jana ; Pospíšil, Lubomil (referee) ; Plánka, Ladislav (advisor)
This master thesis deals development of geographical names, cartographic symbols and on thematically maps documents changes of land use in microregion Lomnicko. Basis for its elaboration were analog and digital topographical and thematically maps from various sources and different time of periods. A part of the thesis are characteristics of used maps documents and conditions of their obtaining. Development of names and cartographic symbols is documented by tables and charts.
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Podnikatelský plán maloobchodu a velkoobchodu kancelářskými potřebami
Domanská, Lucie ; Vojík, Vladimír (advisor) ; Jilková, Jana (referee)
Cílem diplomové práce bylo zjistit povinnosti podnikatelů při zakládání zcela nového podniku, jaká kritéria by měl splňovat, za jakých podmínek může či nemůže svou podnikatelskou činnost provozovat apod. V další části se práce zabývá jednotlivými náležitostmi podnikatelského plánu. Na tyto teoretické poznatky pak navazuje praktická část. Jejím úkolem bylo zjistit, zda autorčin zamýšlený podnikatelský záměr je realizovatelný a z dlouhodobého hlediska také životaschopný a úspěšný.
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