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HPLC in quality control of food supplements containing gingerols and curcumins
Hujová, Eliška ; Šatínský, Dalibor (advisor) ; Chocholouš, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Eliška Hujová Supervisor: prof. RNDr. Dalibor Šatínský, Ph.D. Title of the diploma thesis: HPLC in quality control of food supplements containing gingerols and curcumins In this diploma thesis, a new HPLC method for determination of 6-, 8- and 10-gingerol, 6-shogaol, curcumin, demethoxycurcumin, bisdemethoxycurcumin, and piperine was developed and validated. The developed method was used for the analysis of food supplements: Kurkumin - piperin plus (+ zázvor) from VIESTE, Bio tumeric + ginger from VANAVITA, Ginger root extract from BioMedical, Shogaol zázvor from EPIGEMIC®, Ginger root extract from SWANSON®, and Ginger root (Zingiber officinale) from SOLGAR®. Just as a matter of interest, this method was applied for the selected tea blends containing ginger and/ or tumeric: Dilmah Orange & Ginger (flavoured ceylon black tea), Loyd Brusinky & Zázvor, Teekanne Dýchací cesty a krk se zázvorem, Pickwick Nos a krk se zázvorem, Tesco Ginger & Lemon, Teekanne Ginger Curcuma. For the analysis YMC-Triart C18 ExRS (150 x 4.6 mm; 3 µm) analytical column was used. Separation was carried out by gradient elution with mobile phases A: acetonitrile, B: ultrapure water at flow rate 1 ml/min. Detection was...
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HPLC in quality control of food supplements containing gingerols and curcumins
Hujová, Eliška ; Šatínský, Dalibor (advisor) ; Chocholouš, Petr (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Eliška Hujová Supervisor: prof. RNDr. Dalibor Šatínský, Ph.D. Title of the diploma thesis: HPLC in quality control of food supplements containing gingerols and curcumins In this diploma thesis, a new HPLC method for determination of 6-, 8- and 10-gingerol, 6-shogaol, curcumin, demethoxycurcumin, bisdemethoxycurcumin, and piperine was developed and validated. The developed method was used for the analysis of food supplements: Kurkumin - piperin plus (+ zázvor) from VIESTE, Bio tumeric + ginger from VANAVITA, Ginger root extract from BioMedical, Shogaol zázvor from EPIGEMIC®, Ginger root extract from SWANSON®, and Ginger root (Zingiber officinale) from SOLGAR®. Just as a matter of interest, this method was applied for the selected tea blends containing ginger and/ or tumeric: Dilmah Orange & Ginger (flavoured ceylon black tea), Loyd Brusinky & Zázvor, Teekanne Dýchací cesty a krk se zázvorem, Pickwick Nos a krk se zázvorem, Tesco Ginger & Lemon, Teekanne Ginger Curcuma. For the analysis YMC-Triart C18 ExRS (150 x 4.6 mm; 3 µm) analytical column was used. Separation was carried out by gradient elution with mobile phases A: acetonitrile, B: ultrapure water at flow rate 1 ml/min. Detection was...
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