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Methodology for evaluation of pest resistance to zoocides by biological methods and antiresistance staregies
Kocourek, František ; Stará, Jitka ; Zichová, Tereza ; Hubert, Jan ; Nesvorná, Marta
The methodology contains description of biological methods for evaluation of pest resistance to zoocides arranged according to world standards. The methods are verified on case studies on pests with proved resistance to insecticides or with high risk of selection of resistance in Czechia, as pollen beetle, Colorado potato beetle, cabbage seedpod weevil, cabbage stem flea beetle and codling moth. The methodology contains survey of basic principles of insect resistance management and proposals of antiresistant strategies for pest mentioned in the case studies. The methods for evaluation of resistance are utilized for resistance monitoring performed by state administration or other organisations. Recommendations given in antiresistant strategies are utilized for pest management in winter rape, potatoes and apples.
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The effect of oribatid mites on the composition of fungal community in pine litter
Černá, Kristýna ; Koukol, Ondřej (advisor) ; Hubert, Jan (referee)
4 Abstract The aim of this study was to determine whether oribatid mites affect the variety of fungi in litter of Scots pine (Pinus sylvestris), and if the dispersibility of fungi particles is related to oribatid mite species. To this end, I have prepared several semi-natural systems, in which I observed dispersion of fungi separately and in the presence of two oribatid mite species: Phthiracarus sp. and Adoristes ovatus. I assessed the experiment in two ways, by a cultivation of washed needles on agar medium and direct observation of needles under a binocular magnifier. In the next part of this study I isolated particles of fungi present on the surface and in the faeces of oribatid mites Adoristes ovatus, Porobelba spinosa and Spatiodamaeus verticillipes and compared it with the respective oribatid mite species. The most frequent needles fungi spread by oribatid mites were Oidiodendron maius var. maius, Oidiodendron muniellense, Oidiodendron setiferum, Umbelopsis ramanniana, Mucor hiemalis f. hiemalis and Mortierella hyalina. On the mites body surface the most commonly recorded fungi were Beauveria bassiana, Umbelopsis ramanniana, Cladosporium cladosporioides s.l., Cladosporium herbarum s.l. and unknown species of the genus Penicillium. Fungi species Acrodontium crateriforme, Umbelopsis ramanniana and...
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To first aid wasn't last... Laic first aid with a view to staff in-prison services and denouncement
Kubíková, Helena ; Býma, Svatopluk (advisor) ; Klicperová, Zuzana (referee) ; Hubert, Jan (referee)
Author: Helena Kubíková So the first aid isn't the last one… The non-professional first aid the public with specialization for the employees of prison service and the prisoners The aim of my bachelor's work was to study the employees and convicts in this prison and their level of knowledge in giving the first aid - especially in life threatening situations. I would like to find the problems in this matter and suggest possible solutions. For finding the level of knowledge I decided to use a questionnaire. I formed the questions using the specialized literature providing non-professional first aid by the public. The results showed that both enquired groups need to boost their theoretical knowledge and that they need to get more information. Also the right motivation should be used to encourage a moral duty for helping others. Translate : Petra Hokešová
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Certified technological procedure of laboratory tests of acaricidal compounds against stored product mites
Hubert, Jan ; Nesvorná, Marta ; Stará, Jitka
The certified technological procedure was developed in order to support Czech national legislation - Act No. 258/2000. The procedure is aimed at the protection of stored plant substrates against infestation of mites (Acari: Astigmata). The procedure includes two tested methods (i) impregnated filter paper test and (ii) population growth tests. The procedure includes novel findings describing the discrimination doses of acaricidal compounds to the mites. The doses are useful for identification of resistance. The described methods enable the comparison of efficacy of different acaricidal compounds as well as the comparison of toxic effect to various populations of mites.
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