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Function of gut mucosal barrier: methodology and modulation.
Hornová, Michaela
Function of gut mucosal barrier: methodology and modulation Right function of the intestinal barrier is essential for maintaining the homeostasis of the inner environment and it serves as a mechanical barrier against penetration of macromolecular compounds and microbes from outer environment. Impaired gut mucosa cannot effectively protect from invasion of infectious microorganisms, antigens from food, bacteria, viruses and xenobiotics into the subepithelial gut layer. Excessive interaction among the immune system and antigens from intestinal lumen can lead to immune mediated damage of the organism. Damaged intestinal barrier plays an important role in the pathogenesis of inflammatory and autoimmune diseases, like inflammatory bowel disease, diabetes, celiac disease and rheumatoid arthritis. The aim of this study was to define methods for measuring intestinal permeability, which would reflect the gut barrier damage. We were trying to detect the gut damage caused by indomethacin (non-steroidal anti-inflammatory drug), alcohol and bacterial LPS by measuring the intestinal permeability for 4,4 kDa FITC-dextran. We were also evaluating changes in gene expression of tight junction proteins, IL-6 and iNOS in mice after intraperitoneal administration of LPS, which induces septic shock. Another goal was to...
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Function of gut mucosal barrier: methodology and modulation.
Hornová, Michaela
Function of gut mucosal barrier: methodology and modulation Right function of the intestinal barrier is essential for maintaining the homeostasis of the inner environment and it serves as a mechanical barrier against penetration of macromolecular compounds and microbes from outer environment. Impaired gut mucosa cannot effectively protect from invasion of infectious microorganisms, antigens from food, bacteria, viruses and xenobiotics into the subepithelial gut layer. Excessive interaction among the immune system and antigens from intestinal lumen can lead to immune mediated damage of the organism. Damaged intestinal barrier plays an important role in the pathogenesis of inflammatory and autoimmune diseases, like inflammatory bowel disease, diabetes, celiac disease and rheumatoid arthritis. The aim of this study was to define methods for measuring intestinal permeability, which would reflect the gut barrier damage. We were trying to detect the gut damage caused by indomethacin (non-steroidal anti-inflammatory drug), alcohol and bacterial LPS by measuring the intestinal permeability for 4,4 kDa FITC-dextran. We were also evaluating changes in gene expression of tight junction proteins, IL-6 and iNOS in mice after intraperitoneal administration of LPS, which induces septic shock. Another goal was to...
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Function of gut mucosal barrier: methodology and modulation.
Hornová, Michaela ; Tlaskalová - Hogenová, Helena (advisor) ; Prokešová, Ludmila (referee)
ABSTACT Function of gut mucosal barrier: methodology and modulation Right function of the intestinal barrier is essential for maintaining the homeostasis of the inner environment and it serves as a mechanical barrier against penetration of macromolecular compounds and microbes from outer environment. Impaired gut mucosa cannot effectively protect from invasion of infectious microorganisms, antigens from food, bacteria, viruses and xenobiotics into the subepithelial gut layer. Excessive interaction among the immune system and antigens from intestinal lumen can lead to immune mediated damage of the organism. Damaged intestinal barrier plays an important role in the pathogenesis of inflammatory and autoimmune diseases, like inflammatory bowel disease, diabetes, celiac disease and rheumatoid arthritis. The aim of this study was to define methods for measuring intestinal permeability, which would reflect the gut barrier damage. We were trying to detect the gut damage caused by indomethacin (non-steroidal anti-inflammatory drug), alcohol and bacterial LPS by measuring the intestinal permeability for 4,4 kDa FITC-dextran. We were also evaluating changes in gene expression of tight junction proteins, IL-6 and iNOS in mice after intraperitoneal administration of LPS, which induces septic shock. Another goal was to...
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Composite leading index and its role in the analysis of the business cycle in the Czech republic between 1993-2015
Hornová, Michaela ; Czesaný, Slavoj (advisor) ; Štěpánek, Pavel (referee)
The main topic of my thesis is the problem of monitoring business cycles. Composite leading indicators are one of the options, how to make this monitoring. In my thesis I am going inside of the process of construction of composite leading indicator. I am comparing the methods of OECD, Eurostat and Conference Board. Special attention is dedicated to the methods of czech institutions as well. These methods are still being evolved and improved, which makes the analysis more difficult. Together with all the information about business cycle and economic policy in Czech republic between 1993-2015. I am judgeing the functionality of leading indicators for the monitoring of business cycle. Method of my analysis is SWOT analysis so the result of this thesis is mentioning of the strengths, weaknesses, opportunities and threaths of the making and application of composite leading indicators.
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Gut microbiome and colorectal cancer
Tlaskalová-Hogenová, Helena ; Klimešová, Klára ; Zákostelská, Zuzana ; Kverka, Miloslav ; Hornová, Michaela ; Vannucci, Luca ; Štěpánková, Renata ; Hudcovic, Tomáš ; Kozáková, Hana ; Rossmann, Pavel
The work deals with metagenomic approaches that are currently being used to decipher the genome of the microbiota (microbiome), and, in parallel, functional studies are being performed to analyze the effects of microbiota on the host.
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