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Use of thin layer chromatography for fractionation of humic substances isolated from oxyhumolite
Hegrová, Nela ; Doskočil, Leoš (referee) ; Enev, Vojtěch (advisor)
The main goal of this thesis was to optimize the fractionation of humic substances (HS) using the method of Thin-layer chromatography, so that it can be used for physico-chemical characterization of the obtained individual organic fractions. Due to the high content of HS in the natural oxyhumolite matrix this oxidized brown coal was selected as a source of these biocolloid compounds which were isolated according to the international IHSS procedure. The sample of native oxyhumolite and oxyhumolite after extraction was characterized by thermal techniques such as thermogravimetric (TGA) and elemental analysis (EA). The above-mentioned oxyhumolite samples were further analyzed by Fourier transform infrared spectroscopy, mainly for deeper understanding of their structure in terms of an organic matrix containing HS. Characterization of isolated HSs was performed by the following thermochemical and spectrometric techniques, elemental analysis, thermogravimetry, ultraviolet and visible electromagnetic absorption (UV/Vis) molecular spectroscopy, Fourier transform infrared spectroscopy and steady-state fluorescence spectroscopy. Molecular absorption spectroscopy (UV/Vis) and steady-state fluorescence spectroscopy were used for physicochemical characterization of individual organic fractions.
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Application of thin layer chromatography (TLC) for fractionation of humic substances.
Hegrová, Nela ; Doskočil, Leoš (referee) ; Enev, Vojtěch (advisor)
The main aim of this Bachelor thesis was to suggest and optimize a method of fractionation of organic matter of humic substances using thin-layer chromatography (TLC). HS Pahokee Peat standards have been characterized by thermochemical and spectrometric techniques such as thermogravimetry, elemental analysis, UV-Vis and Fourier-transform infrared spectrometry. Molecular absorption spectrometry in the ultraviolet and visible range of electromagnetic radiation and steady-state fluorescence spectrometry were used for physicochemical characterization of organic fractions. Using fluorescence spectrometry, humic substances were found to be composed of fluorophores of humic and non-humic (protein) character. Organic fractions corresponding to the humic fluorphores were characterized by a higher aromaticity, average molecular weight and higher content of oxygen substituents on the aromatic nucleus. In contrast, the organic fractions corresponding to the protein constitutional units were distinguished by low values of the humification index. These tell us about microbial origin of these organic substances during the genesis of HS Pahokee Peat.
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Use of thin layer chromatography for fractionation of humic substances isolated from oxyhumolite
Hegrová, Nela ; Doskočil, Leoš (referee) ; Enev, Vojtěch (advisor)
The main goal of this thesis was to optimize the fractionation of humic substances (HS) using the method of Thin-layer chromatography, so that it can be used for physico-chemical characterization of the obtained individual organic fractions. Due to the high content of HS in the natural oxyhumolite matrix this oxidized brown coal was selected as a source of these biocolloid compounds which were isolated according to the international IHSS procedure. The sample of native oxyhumolite and oxyhumolite after extraction was characterized by thermal techniques such as thermogravimetric (TGA) and elemental analysis (EA). The above-mentioned oxyhumolite samples were further analyzed by Fourier transform infrared spectroscopy, mainly for deeper understanding of their structure in terms of an organic matrix containing HS. Characterization of isolated HSs was performed by the following thermochemical and spectrometric techniques, elemental analysis, thermogravimetry, ultraviolet and visible electromagnetic absorption (UV/Vis) molecular spectroscopy, Fourier transform infrared spectroscopy and steady-state fluorescence spectroscopy. Molecular absorption spectroscopy (UV/Vis) and steady-state fluorescence spectroscopy were used for physicochemical characterization of individual organic fractions.
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Application of thin layer chromatography (TLC) for fractionation of humic substances.
Hegrová, Nela ; Doskočil, Leoš (referee) ; Enev, Vojtěch (advisor)
The main aim of this Bachelor thesis was to suggest and optimize a method of fractionation of organic matter of humic substances using thin-layer chromatography (TLC). HS Pahokee Peat standards have been characterized by thermochemical and spectrometric techniques such as thermogravimetry, elemental analysis, UV-Vis and Fourier-transform infrared spectrometry. Molecular absorption spectrometry in the ultraviolet and visible range of electromagnetic radiation and steady-state fluorescence spectrometry were used for physicochemical characterization of organic fractions. Using fluorescence spectrometry, humic substances were found to be composed of fluorophores of humic and non-humic (protein) character. Organic fractions corresponding to the humic fluorphores were characterized by a higher aromaticity, average molecular weight and higher content of oxygen substituents on the aromatic nucleus. In contrast, the organic fractions corresponding to the protein constitutional units were distinguished by low values of the humification index. These tell us about microbial origin of these organic substances during the genesis of HS Pahokee Peat.
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