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Methodology for co-cryostorage of virus isolates in their host plants and relevant data
Bilavčík, A. ; Bjerkestrand, R. ; Bjelland, B. ; Blystad, D.-R. ; Bobrova, O. ; Fránová, Jana ; Faltus, M. ; Hamborg, Z. ; Hammond Hammond, S. D. ; Koloniuk, Igor ; Zámečník, J.
Currently, the nursery production of plant material emphasizes the production of healthy, virus-free material. For this reason, it is necessary to have reference plant materials in which the virus is present. Some types of viruses are difficult to maintain outside the plant host. Because of the potential transmission to outdoor plantings, it is necessary to keep infected plants in special cultivation areas, and net-houses, which brings considerable financial and labour costs. Therefore, a methodology was developed in which the virus status in plant material is analysed, the infected material can be stored using cryopreservation in liquid nitrogen and then regenerated in vitro, and relevant data in GeneEver cryobox can be stored.
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