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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Optimisation of rapeseed in vitro cultures used for CRISPR/Cas9 technology
 HARENČÁK, Jan
Plant transformation using Agrobacterium tumefaciens bacteria (Rhizobium radiobacter, according to new nomenclature) is widely used method for plant genome editing. However, different oilseed rape varieties do not always have the same in vitro regeneration capacity, which is crucial for transformation. This work aims to optimize regeneration protocol for selected varieties of oilseed rape. Of the six varieties tested, only two regenerated successfully - Arabella and Obelix. The created protocol can be used for regeneration without transformation, but it is also possible to implement specific steps in it that enable transformation using A. tumefaciens.
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Use of RT-PCR in stress reaction studies
 HARENČÁK, Jan
The aim of this work is to evaluate the expression of the ERD10 gene for dehydrin protein and ICE1 transcription factor gene in oilseed rape, which are potentially connected to the cold stress reaction in oilseed rape. Eventhough the oilseed rape is relatively cold resistant, cold stress is an significant element reducing its yield in the Czech Republic. The analysis of expression was done using RT qPCR method in cold stressed plants of Cadeli and Navajo varieties which both show a different cold stress reaction.
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