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Sequential injection chromatography - advanced and two-dimensional separations
Gužiňák, Matej ; Chocholouš, Petr (advisor) ; Šatínský, Dalibor (referee)
Department: Department of analytical chemistry Candidate: Matej Gužiňák Supervisor: doc. PharmDr. Petr Chocholouš, Ph.D. Title of the diploma thesis: Sequential injection chromatography - advanced and two- dimensional separations The subject of this work was the development of two analytical methods intended for separating substances contained in silymarin, their comparison and preparing a template in the Microsoft Excel program to simplify data evaluation. In the first developed method, called classical sequential injection chromatography (1D- SIC), a long Ascentis® Express F5 chromatography column (10 cm x 4.6 mm, 5 µm) with an opti-guard® C18 pre-column (1 mm) was used during analysis. The mobile phase consisted of a mixture of acetonitrile with phosphoric acid solution with pH 2.0 prepared in a ratio of 27:73. The isocratic mode was chosen for the analysis; the total length was 16.3 minutes with a flow rate of 5 μL/s. In the second developed method, called two-dimensional sequential injection chromatography (2D-SIC), a Kinetex® 5 µm EVO C18 column (30 x 3.0 mm) with an opti- guard® C18 pre-column (1 mm) was chosen for the first dimension. In the second dimension, an Ascentis® Express F5 column (30 mm x 4.6 mm, 2.7 µm) was used. The analysis mode was chosen isocratic with a total length of 35.3...
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