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Rare B lymphocyte populations in the context of peripheral B lymphopoiesis in humans
Grígelová, Andrea ; Růžičková, Šárka (advisor) ; Hrušák, Ondřej (referee)
B-cells of peripheral blood in humans represent a heterogeneous cellular environment displaying many important functions in the immune system. Recently, there is an increasing amount of evidence that B cell subpopulations are involved in the pathogenesis of many different diseases. However, there is little or no knowledge on how the individual differentiation stages of B lymphocytes are involved in pathological processes, and how they are distributed and represented under the physiological state and under pathological conditions. There is a reasonable assumption that, as with dendritic cells, NK / NKT cells and T lymphocytes, also B cell populations will contain minor and/or rare subpopulations reaching relative frequencies in the range of 0.01% to 0, 1 ‰. The primary aim of this thesis was to investigate the extent of phylogenetic and ontogenetic heterogeneity of the peripheral B cell population and lymphopoietic tissues on the basis of a comparative study across different vertebrate species. Another goal of the work was to use polychromatic flow cytometry with 183 individuals, out of them 50 controls and 133 patients with different (immune) pathologies or tumors in order to identify an optimal combination of surface features, and to use it to detect and demonstrate the existence of minor/rare...
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Academic spin-off companies in the Czech Republic
Grígelová, Andrea ; Patěk, Daniel (advisor) ; Štenglová, Ivanka (referee)
Academic spin-off companies in the Czech Republic My personal interest in understanding the mechanisms and structures of academic society was the reason for choosing the topic. The issue of academic entrepreneurship the author met during my employment at Palacky University. In the course of work related to the preparation of internal documents regulating the Palacky University shareholding in the business of other legal persons, I faced many problems that emanated from a specific college status as legal persons under public law and traits that show business companies in which the partial ownership belongs to a research organisation. Due to the fact that the topic is completely untreated in the Czech literature, I decided to compile my findings on the functioning of academic spin-off companies in the thesis. The thesis is divided into five content bearing chapters (total work has 10 chapters). The first chapter contains the introduction. The second chapter is divided into three parts and deals with the definition of the concept, types and models of formation of academic spin-off companies. The third chapter describes the historical background and the context of academic spin-off companies' creation in the United States, Europe and the Czech Republic with an emphasis on the interaction between the academic...
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