|
|
The role of Hepatitis B virus capsid protein in the host ubiquitin proteasome pathway
Eliáš, Vratislav ; Weber, Jan (advisor) ; Šmahel, Michal (referee)
Hepatitis B virus (HBV) is a Hepadnaviridae virus infecting mammals. Its infection can result in an acute or chronic infection. Chronic infection can result in hepatocellular carcinoma and liver cirrhosis, potentially leading to death of the patient. HBV is a small 42 nm virus with a genome length of 3.2 kb encoding seven viral proteins. HBV Core protein (HBc) is a capsid forming protein which is pleiotropic in function. We have identified two ubiquitin ligases which could interact with this protein: F-box only protein 3 (FBXO3; E3 ubiquitin ligase) and Ubiquitin conjugating enzyme E2 O (UBE2O; E2/E3 ubiquitin ligase). By employing multiple methods we have confirmed these interactions. Co- immunoprecipitation and further western blot analysis unveiled multiple new insights into the ligases′ impact on HBc: FBXO3-mediated HBc polyubiquitination stimulation and UBE2O-mediated HBc monoubiquitination promotion. FBXO3's and UBE2O's role in HBV life cycle was investigated as well. By silencing the expression of FBXO3 and UBE2O respectively, we have observed changes in HBV replication levels: FBXO3 serves as an inhibitor of HBV replication, while UBE2O stimulates the course of HBV life cycle. Further investigation of these newly-discovered understandings may lead to a whole new HBV - host interplay...
|
|
|
The role of capsid protein in hepatitis B virus life cycle
Eliáš, Vratislav ; Weber, Jan (advisor) ; Drda Morávková, Alena (referee)
Hepatitis B virus (HBV) can cause either an acute or a chronic infection of hepatocytes, often leading to a hepatocellular carcinoma or a liver cirrhosis. HBV encodes seven proteins in its 3.2kb genome. Two of these proteins are transcribed from the same ORF (HBcAg and HBeAg). HBeAg, as a soluble variant of the core protein, plays a crucial role in virus immunogenicity. HBcAg, apart from building viral capsid, represents a pleiotropic protein engaging in multiple viral stages. Its primary structure is divided into two domains: an assembly domain and a C-terminal domain (CTD). The latter, containing four arginine clusters, is an important life cycle regulation element. By interaction with multiple viral and cellular factors, it directs the virus through a successful replication. In this thesis, we have collected the available data about the various roles of HBcAg in viral life cycle. We believe that further investigation of this field could lead to therapeutic advances resulting in the decrease of HBV infections worldwide, subsequently to a lower lethality connected with the diseases caused by HBV. Powered by TCPDF (www.tcpdf.org)
|
|
|
The role of Hepatitis B virus capsid protein in the host ubiquitin proteasome pathway
Eliáš, Vratislav ; Weber, Jan (advisor) ; Šmahel, Michal (referee)
Hepatitis B virus (HBV) is a Hepadnaviridae virus infecting mammals. Its infection can result in an acute or chronic infection. Chronic infection can result in hepatocellular carcinoma and liver cirrhosis, potentially leading to death of the patient. HBV is a small 42 nm virus with a genome length of 3.2 kb encoding seven viral proteins. HBV Core protein (HBc) is a capsid forming protein which is pleiotropic in function. We have identified two ubiquitin ligases which could interact with this protein: F-box only protein 3 (FBXO3; E3 ubiquitin ligase) and Ubiquitin conjugating enzyme E2 O (UBE2O; E2/E3 ubiquitin ligase). By employing multiple methods we have confirmed these interactions. Co- immunoprecipitation and further western blot analysis unveiled multiple new insights into the ligases′ impact on HBc: FBXO3-mediated HBc polyubiquitination stimulation and UBE2O-mediated HBc monoubiquitination promotion. FBXO3's and UBE2O's role in HBV life cycle was investigated as well. By silencing the expression of FBXO3 and UBE2O respectively, we have observed changes in HBV replication levels: FBXO3 serves as an inhibitor of HBV replication, while UBE2O stimulates the course of HBV life cycle. Further investigation of these newly-discovered understandings may lead to a whole new HBV - host interplay...
|
|
|
The role of capsid protein in hepatitis B virus life cycle
Eliáš, Vratislav ; Weber, Jan (advisor) ; Drda Morávková, Alena (referee)
Hepatitis B virus (HBV) can cause either an acute or a chronic infection of hepatocytes, often leading to a hepatocellular carcinoma or a liver cirrhosis. HBV encodes seven proteins in its 3.2kb genome. Two of these proteins are transcribed from the same ORF (HBcAg and HBeAg). HBeAg, as a soluble variant of the core protein, plays a crucial role in virus immunogenicity. HBcAg, apart from building viral capsid, represents a pleiotropic protein engaging in multiple viral stages. Its primary structure is divided into two domains: an assembly domain and a C-terminal domain (CTD). The latter, containing four arginine clusters, is an important life cycle regulation element. By interaction with multiple viral and cellular factors, it directs the virus through a successful replication. In this thesis, we have collected the available data about the various roles of HBcAg in viral life cycle. We believe that further investigation of this field could lead to therapeutic advances resulting in the decrease of HBV infections worldwide, subsequently to a lower lethality connected with the diseases caused by HBV. Powered by TCPDF (www.tcpdf.org)
|
|
|
Marketing communication (demonstrated on hotel Golf Praha)
Kadlecová, Alena ; Indrová, Jarmila (advisor) ; Eliáš, Vratislav (referee)
Marketing communication (demonstrated on hotel Golf Praha) The aim of this thesis is to define criteria of marketing communication in the field of hospitality. One part of the thesis is focused on evaluation of hotel Golf Praha communication according to its quality and efficiency. Benefits should be definitely eventual suggestions for improving the communication and its quality, or changes that should actuate on clients as effectively as possible to help reach better commercial results.
|
guest ::
RSS feed.