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Explant culture of Trifolium pratense L.
Dudová, Adriana ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Adriana Dudová Supervisor: PharmDr. Marie Kašparová, PhD. Title of Doctoral Thesis: Explant Culture of Trifolium pratense L. The objective of this thesis was to observe the effect of the abiotic elicitors of aluminium chloride and chromium chloride on the production of flavonoids, as well as the effect of the aluminium chloride elicitor on the production of isoflavonoids in the Trifolium pratense L. exulant culture (variety DO-9). The cultures were cultivated in the Gamborg nutrient media with the addition of 2 mg.l¯¹ of 2,4-dichlorophenoxyacetic acid and 2 mg.l¯¹ of 6-benzylaminopurine, at the temperature of 25 řC, 16-hr light/8-hr dark period. The quantity of flavonoids was determined spectrophotometrically according to the Czech Pharmacopoeia 2009. The quantity of isoflavonoids was determined using HPLC. The results of the elicitation of the Trifolium pratense L. suspension culture with aluminium chloride and chromium chloride show that the maximum increase in the flavonoid production compared to the control culture was evoked by the application of a 100 µmol concentration over a 168-hr period in both cases. During elicitatiton of the Trifolium pratense L. exulant culture (variety DO-9)...
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Spirulina platensis effects in experimental atherogenesis
Dudová, Adriana ; Nachtigal, Petr (advisor) ; Rathouská, Jana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Spirulina platensis effects in experimental atherogenesis Rigous thesis Mgr. Adriana Dudová Consultant of rigous thesis: PharmDr. Petr Nachtigal, Ph.D. Spirulina platensis is a blue-green microalga belonging to the cyanobacteria family. Microalga is a large source of proteins and good fatty acid and other nutritional elements, for example calcium, iron, chromium, lithium, selenium. It also contains natural dyes chlorophyll and phycocyanin. Its antioxidant and anti-inflammatory were described. Background: In this rigous thesis, was to determine potencial anti-inflammatory effects of Spirulina platensis in apoE-deficient mice. Methods: In this study were used ApoE knockout mice. ApoE-deficient mice were fed a standard diet for ten weeks. Mice were randomly divided in to two groups. One group (n=8) wasfed a diet containing 1% cholesterol (control group). The other group (Spirulina group) received the same atherogenic diet only enriched with Spirulina platensis at the dosage of 40 mg per day on the mouse. The biochemical analysis of total cholesterol of blood sample was performed and expression of macrophages in atherosklerotic lesion was determined by imunohistochemical and stereological...
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Explant culture of Trifolium pratense L.
Dudová, Adriana ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Adriana Dudová Supervisor: PharmDr. Marie Kašparová, PhD. Title of Doctoral Thesis: Explant Culture of Trifolium pratense L. The objective of this thesis was to observe the effect of the abiotic elicitors of aluminium chloride and chromium chloride on the production of flavonoids, as well as the effect of the aluminium chloride elicitor on the production of isoflavonoids in the Trifolium pratense L. exulant culture (variety DO-9). The cultures were cultivated in the Gamborg nutrient media with the addition of 2 mg.l¯¹ of 2,4-dichlorophenoxyacetic acid and 2 mg.l¯¹ of 6-benzylaminopurine, at the temperature of 25 řC, 16-hr light/8-hr dark period. The quantity of flavonoids was determined spectrophotometrically according to the Czech Pharmacopoeia 2009. The quantity of isoflavonoids was determined using HPLC. The results of the elicitation of the Trifolium pratense L. suspension culture with aluminium chloride and chromium chloride show that the maximum increase in the flavonoid production compared to the control culture was evoked by the application of a 100 µmol concentration over a 168-hr period in both cases. During elicitatiton of the Trifolium pratense L. exulant culture (variety DO-9)...
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