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National Repository of Grey Literature 4 records found  Search took 0.01 seconds. 
The role of glycoconjugates in cell signalling - CEACAM1 and Fc ε RI
 Dudková, Lenka
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The role of glycoconjugates in cell signalling - CEACAM1 and Fc ε RI
 Dudková, Lenka
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The role of glycoconjugates in cell signalling - CEACAM1 and Fc ε RI
 Dudková, Lenka ; Šmíd, František (advisor) ; Stiborová, Marie (referee) ; Bílková, Zuzana (referee)
Carcinoembryonic cell adhesion molecule 1 (CEACAM1) is human membrane glycoprotein. The purpose of the first part of this thesis was to isolate CEACAM1 glycoprotein from bile and characterise its purity and recovery that have not been described before. Affinity chromatography of CEACAM1 on hydrazide-activated cellulose with immobilized monoclonal anti-CEA F34-187 antibodies is described. The method is based on the oxidation of the immunoglobulin carbohydrate component located on the Fc fragment of antibody by periodate and oriented bounding to hydrazide-activated matrix. Crude protein fraction from bile was applied onto the affinity column and CEACAM1 was eluted with 6 M guanidine-HCl. A single immunopositive 85 kDa band of CEACAM1 was detected on the western blot with anti-CEA antibody. Probably due to the high glycosylation of CEACAM1 any common method of protein staining was not applicable except staining with lectin.
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Czech-German Economic Relations
 Dudková, Lenka ; Čajka, Radek (advisor) ; Richter, Stanislav (referee)
The aim of this thesis is to evaluate the economic relations between the Czech Republic and Germany by analysing their economies, foreign trade policies and mutual economic cooperation. The first chapter presents and compares the basic macroeconomic data. The following chapter focuses on the history of mutual relations, the Czech-German merchandise and services trade and the potential of mutual trade relations. The last chapter is devoted to the foreign direct investments in both economies.
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3 DUDKOVÁ, Lucie
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