National Repository of Grey Literature 48 records found  1 - 10nextend  jump to record: Search took 0.00 seconds. 
Inhibition of nicotinic acetylcholine receptors by tacrine and its derivatives
Skřenková, Kristýna ; Krůšek, Jan (advisor) ; Doležal, Vladimír (referee)
Nicotinic acetylcholine receptors are ligand-gated ion channels which are located on neuromuscular junction and in central and perifric nervous system. Activity of nicotinic receptor might be modulated by variety of pharmacological agents. In this work, we have focused on the study of the inhibition effect of tacrine and its derivatives on the nicotinic acetycholine receptors of muscle and neuronal type. These derivatives function as acetylcholinesterase inhibitors and also interact with nicotinic acetylcholine receptors. The majority of current forms of treatment of Alzheimer's disease is based on cholinesterase inhibitors. We have studied the mechanism of tacrine and its derivatives by using patch clamp method in the configuration of whole-cell recording. Powered by TCPDF (www.tcpdf.org)
Gen expresion of high affinity choline transporter in mouse model of Alzheimer's disease
Kurfürst, Helena ; Doležal, Vladimír (advisor) ; Krištofíková, Zdena (referee)
: Choline is being used in all mammalian cells as a precursor for synthesis of a major phospholipide phosphatidylcholine and as a donor of acetyl residues. Cholinergic neurons in addition require choline to synthesize neuromediator acetylcholine. The ability of cells to create choline via de novo synthesis is limited and therefore they need to transport choline from extracellular space. Limited availability of choline in brain leads specifically to diminished function of cholinergic neurons and in general to impaired reparation of biological membranes. Dysfunctions of cholinergic signaling in brain is characteristic for Alzheimer's disease. Aim of this work was to investigate whether gene and protein expression of high- affinity cholinergic transporters is altered in 5-6 months old APPswe/PS1dE9 mouse model of Alzheimer's disease. Expression of specific high-affinity cholinergic transporter CHT1 (responsible for transport of choline to be used for acetylcholine synthesis) and putative high-afinity choline transporter CTL1 (generally present in all cells and related to high affinity choline transport for phospholipide synthesis) in cerebral cortex was measured. Compared to non-trangenic littermates, no changes in the expression of both genes were detected at either mRNA (quantitative PCR) or protein...
Cholinergic neurons and Alzheimer's disease
Machová, Eva ; Doležal, Vladimír (advisor) ; Krištofíková, Zdena (referee) ; Zvolský, Petr (referee)
Alzheimer's disease is a degenerative brain disorder. The incidence of the disease increases with age and every year the total number of affected persons is higher. The malfunction of various mental functions is a typical feature of Alzheimer's disease, including loss of short-term memory and development of personality changes. It is now generally accepted that the main cause of the disease is increased production of r3-amyloid fragments that mediate toxic effects and lead to r3-amyloid plaques formation. Alzheimer's disease and also normal aging are accompanied by a loss of cholinergic neurons and weakened cholinergic neurotransmission. In my thesis I dealt with changes in the brain chol inergic system during aging in control mice and in a mouse model of Alzheimer's disease (APPswe/PS1 dE9). In this context I also investigated in vitro influence of docosahexaenoic acid (w-3 essential fatty acid) changes in membrane cholesterol content on the expression of cholinergic phenotype in the NG108-1 5 cholinergic cell line. I also performed ex vivo experiments on rat brain cortex to investigate the characteristics of action of muscarin ic agonist xanomeline that was developed as a selective muscarinic agonist to strengthen signalization through the muscarinic M1 receptor in Alzheimer's disease. The experiments on...
The role of charged residues in the activation and modulation of the TRPA1 ion channel
Zímová, Lucie ; Vlachová, Viktorie (advisor) ; Doležal, Vladimír (referee) ; Rokyta, Richard (referee)
Important receptor for sensing painful stimuli is ion channel TRPA1, which is expressed in peripheral endings of nociceptive neurons, where it serves as transducer of physical and chemical environmental signals to the language of the nervous system. The effort to understand the mechanisms of its activity on a molecular level is driven by the vision of progress in treatment of chronic pain in humans. Our work focused on C-terminal cytoplasmic domain of TRPA1 receptor, where we described i.a. the probable binding site for calcium, which is the most important TRPA1 modulator. Using the combination of homology modeling and molecular dynamic simulations with electrophysiological measurements we were able to explain molecular basis of familial pain syndrome caused by TRPA1 point mutation. We contributed to the understanding of the TRPA1 voltage-dependent activation mechanism by describing the amino acids in proximal C-terminus and in S4-S5 linker of transmembrane domain that are directly involved in voltage-dependent gating. Powered by TCPDF (www.tcpdf.org)
My experiences with interpretation of Gustav Mahler´s work
Vondrová, Petra ; HAJÓSSYOVÁ, Magdaléna (advisor) ; DOLEŽAL, Vladimír (referee)
In my bachelor thesis I have decided to focus on the person of Gustav Mahler. A large part of the text is focused on his fate a works. After reviewing important events from his life, I´m watching his professional development. In conclusion I am focusing on his work Kindertotenlieder - I am capturing the history of this work´s origin, form and its structure. Using a comparation method I am also watching their interpretation by famous singer Dietrich Fischer- Dieskau as a guide for my own interpretation. I am also describing my own problems with their interpretation. Their solution have rised my experiences.
Comparison of Leoncavallo’s and Puccini’s opera La Bohéme
Škandera, Adam ; DOLEŽAL, Vladimír (advisor) ; MELNIK, Natalija (referee)
Bachelor thesis focuses on important figures of the great opera world of the 19th and 20th centuries, Giacomo Puccini and Rugger Leoncavallo. It focuses on the life of both composers and a summary of their opera works. Great emphasis is placed above all on La Bohéme opera, which the two composers wrote almost simultaneously. The work describes the story of both of Bohém, which, although written on the same subject of the novel by Henry Murger Scènes de la Vie de Boheme, are different and each highlights the other components of the story.

National Repository of Grey Literature : 48 records found   1 - 10nextend  jump to record:
See also: similar author names
1 DOLEŽAL, Vladislav
5 DOLEŽAL, Václav
3 Doležal, Vojtěch
5 Doležal, Václav
4 Doležal, Vít
2 Doležal, Vítězslav
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