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Relation between cardiovascular microRNAs and pregnancy-related complications
Dalecká, Linda ; Hromadníková, Ilona (advisor) ; Daňková, Pavlína (referee)
During the pregnancy, there are many complications, which can affect a fetal development and eventually, its or mother's life. Pre-eclampsia is one of these complications. It is a hypertensive disorder, which appears during the pregnancy. Another example of these complications is an intrauterine growth restriction. It is a condition, when the fetus is not able to reach its genetical growth potential. Both of those pathological disorders are accompanied by changes in microRNA gene expression in placenta, for example miR-16, miR-21, miR-210. Knowledge about these changes in gene expression could represent unique instruments in the field of noninvasive prenatal diagnosis of these disorders. This could be benefitial due to the fact, that both of these disorders are responsible for increasing maternal and perinatal morbidity and mortality. This work focused on microRNAs, their expression in aformentioned disorders and benefits, which could bring in diagnostics. In this work these two disorders, characteristics of microRNAs and their biogenesi are described. MicroRNAs are interesting for us as potential biomarkers for noninvasive prenatal diagnostics becouse they are present in body fluids, such as plasma, breast milk or amniotic fluid and they are not subject of rapid degradation. Key words:...
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Eda gene in the development of teeth
Dalecká, Linda ; Hovořáková, Mária (advisor) ; Buchtová, Marcela (referee)
Ectodysplasin A (EDA) is a transmembrane protein of the TNF family, which plays an important role in the development of ectodermal derivates, such as teeth, hair or glands. Mutation in Eda gene causes the Hypohidrotic ectodermal dysplasia (HED) in humans. Sonic hedgehog (Shh) as a downstream of Eda signalling pathway is an important signalling molecule involved in the initiation of tooth development. In frame of the present study, we aimed to evaluate the involvement of Eda gene during the development of mice teeth and its relation to Shh signalling, since Shh is an important marker of the normal tooth development. We focused on Shh expression in Eda mutant mice teeth with spontaneous mutation in Eda gene, since these mice represent a natural model for X-linked HED. First, we compared the Shh expression pattern during the tooth development in Eda mutant mice with CD1 control using the dissociation of dental epithelia and fluorescent microscopy. Consequently, we focused on the cell line expressing Shh observation using Cre-loxP system. We also visualized SHH expression in Eda mutants using imunohistochemistry and qantified SHH protein in the embryonic jaws using western blot. According to our results, the development of teeth in Eda mutants seems to be approximately one day delayed compared to...
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Eda gene in the development of teeth
Dalecká, Linda ; Hovořáková, Mária (advisor) ; Buchtová, Marcela (referee)
Ectodysplasin A (EDA) is a transmembrane protein of the TNF family, which plays an important role in the development of ectodermal derivates, such as teeth, hair or glands. Mutation in Eda gene causes the Hypohidrotic ectodermal dysplasia (HED) in humans. Sonic hedgehog (Shh) as a downstream of Eda signalling pathway is an important signalling molecule involved in the initiation of tooth development. In frame of the present study, we aimed to evaluate the involvement of Eda gene during the development of mice teeth and its relation to Shh signalling, since Shh is an important marker of the normal tooth development. We focused on Shh expression in Eda mutant mice teeth with spontaneous mutation in Eda gene, since these mice represent a natural model for X-linked HED. First, we compared the Shh expression pattern during the tooth development in Eda mutant mice with CD1 control using the dissociation of dental epithelia and fluorescent microscopy. Consequently, we focused on the cell line expressing Shh observation using Cre-loxP system. We also visualized SHH expression in Eda mutants using imunohistochemistry and qantified SHH protein in the embryonic jaws using western blot. According to our results, the development of teeth in Eda mutants seems to be approximately one day delayed compared to...
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Relation between cardiovascular microRNAs and pregnancy-related complications
Dalecká, Linda ; Hromadníková, Ilona (advisor) ; Daňková, Pavlína (referee)
During the pregnancy, there are many complications, which can affect a fetal development and eventually, its or mother's life. Pre-eclampsia is one of these complications. It is a hypertensive disorder, which appears during the pregnancy. Another example of these complications is an intrauterine growth restriction. It is a condition, when the fetus is not able to reach its genetical growth potential. Both of those pathological disorders are accompanied by changes in microRNA gene expression in placenta, for example miR-16, miR-21, miR-210. Knowledge about these changes in gene expression could represent unique instruments in the field of noninvasive prenatal diagnosis of these disorders. This could be benefitial due to the fact, that both of these disorders are responsible for increasing maternal and perinatal morbidity and mortality. This work focused on microRNAs, their expression in aformentioned disorders and benefits, which could bring in diagnostics. In this work these two disorders, characteristics of microRNAs and their biogenesi are described. MicroRNAs are interesting for us as potential biomarkers for noninvasive prenatal diagnostics becouse they are present in body fluids, such as plasma, breast milk or amniotic fluid and they are not subject of rapid degradation. Key words:...
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