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BUT Area Kraví hora
Trčálková, Miroslava ; Dvořák, Miloš (referee) ; Nový, Alois (advisor) ; Dvořák, Miloš (advisor)
The aim of the diploma thesis is a detailed study of the VUT area at Kraví Hora to a scale of 1: 200. The aim is to combine the interests of VUT and Brno in this extraordinary territory for the benefit of both subjects. For the needs of the city, I created a publicly accessible park in which I placed the VUT museum buildings and the spaces for the promotion of individual VUT faculties that the Technical University lacks. I have also placed the object of the creative business center here. These are rented spaces especially for VUT graduates and VUT employees who want to do creative work. Both buildings with a green walkable roof are embedded in the terrain so as not to disturb the overall impression of the landscape and create a natural transition between the buildings and the city park.
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Probiotics and probiotic microorganisms
Kostková, Lucie ; Dvořák, Miloš (referee) ; Babák, Libor (advisor)
Probiotics and their positive influence on human health are presently very discussed theme. Nevertheless is informedness of public about probiotics benign influence insufficient, as well as their practical usage. This thesis summarizes present scientific knowledge about probiotic effects on human organismus. Fisrt chapter deals with determination of concept probiotics, their history, meaning, qualities, effects on human health and hazard connected with probiotic usage. Second chapter includes description of probiotic products, their forms and foodstuff containing probiotics. Chapter three discuss probiotic microorganisms, their main characteristics and examples of commercial starting cultures. Fourth chapter takes in informations about viability of probiotic microorganisms. And last chapter researches methods of integration of probiotics into products.
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Study of the authenticity of chocolate with different content of cocoa solids
Vanduchová, Petra ; Dvořák, Miloš (referee) ; Diviš, Pavel (advisor)
This master's thesis deals with authenticity of chocolate with different content of cocoa solids. The theoretical part describes the production of chocolate products and the issue of food authenticity. Experimental part of this thesis is focused on the determination of total cocoa solids in the selected samples. Different samples of chocolate containing 50–90 % of cocoa were grated to fine particles, that were subjected to extraction without previous chemical treatment. The methylxanthines theobromine and caffeine were detected by HPLC to determine the content of fat–free cocoa solids. Fat–free cocoa solids content were found in the range 19,7–43,1 g/100 g of dry matter and the content of total fat was determined by the gravimetric method and was found in the range 30,9–55,4 g/100 g. The results of this thesis prove that, the producers meet the legislative requirements and the declared values are corectly declared on the wrappers of the product.
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Study on pretreatment and hydrolysis of selected lignocellulose materials
Kovářová, Markéta ; Dvořák, Miloš (referee) ; Obruča, Stanislav (advisor)
This diploma thesis is focused on study of chemical and enzymatical hydrolysis of raw wood material. The aim of this work was to find the suitable method for pretreatment of selected lignocellulose materials. The theoretical part deals with characterization of lignocellulosic material and its components. There are also subscribed various pretreatment methods and their effect on hydrolysis of sawdust. In experimental part of the work the most appropriate approach of pretreatment and hydrolysis of sawdust was studied. Criteria for the selection of suitable method was concentration of saccharides as desired product of hydrolysis and also concentration of the most important microbial inhibitors - polyphenols. Application of 96% ethanol or 5% H2O2 were identified as the most promising pretreatment methods which enhanced yields of fermentable sugars about 30 %. Further, we also performed cultivation of bacteria Burkholderia cepacia and bacteria Burkholderia sacchari using solution obtained by hydrolysis of lignocellulose material.
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Low-cost microextraction and preconcentration procedures for biomedical applications
Vašátko, Jan ; Diviš, Pavel (referee) ; Dvořák, Miloš (advisor)
This thesis focuses on low-cost microextraction techniques and their application for purification and preconcentration of biological samples, specifically on the experimental study of supported liquid membrane (SLM) extraction. The described microextraction technique uses commercially available filtration plates as the extraction units and allows the extraction of basic drugs from biological samples of urine and blood (in the form of dried blood spots). The experimental part includes the optimization of microextraction conditions of basic drugs from real samples through a SLM coupled in-line to lab-made capillary electrophoresis. The basic optimization of microextraction conditions involved selecting the appropriate organic phase for membrane impregnation (1:1 mixture of ENB and DHE), appropriate agitation speed for sample convection during extraction (1000 rpm), and optimal ratio of donor to acceptor volumes for high preconcentration of the analytes (400:15 µL). After basic optimization, the effect of donor alkalization with NaOH on extraction recovery (ER) was investigated. For all matrices used (saline solution, undiluted human urine samples, human capillary blood eluted from dry blood spots with deionized water), the highest ER values were achieved using a neutral donor and an acidic acceptor. The extraction time (60 minutes) was optimized based on the time profile of the microextraction for 120 minutes. This optimized microextraction method is suitable for the determination of basic drugs in real matrices with sufficient sample clean-up, preconcentration and ER values.
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BUT Area Kraví hora
Muroň, Jakub ; Dvořák, Miloš (referee) ; Nový, Alois (advisor)
The subject of this diploma thesis is to continue on the previous atelier work, which was focused on elaboration of a strategic urban-architectural study of the Kraví hora area and the BUT holding located there. A multifunctional facility, which would include activities that BUT still lacks, should be designed there. In any case, it should not be an extension of the teaching, research or accommodation capacities, since there have been built enough of these by BUT recently. At the same time, the proposed activities should not be controversial with the interests of the city. The BUT area at Kraví hora is one of the most valuable areas of the city of Brno. The city, which has tried to buy it from BUT several times, has an eminent interest in this area. However BUT considers it as its "family silver", because they realize its extraordinary value. Nevertheless, due to the binding regulatory conditions, construction possibilities are very limited. The aim of this study is to find such a use of this area, which would be a compromise corresponding to both the city's expectations and the needs of BUT. The intention of the previous project, which has been continued by this diploma thesis, was the removal of unsatisfactory buildings and the reduction of the garden colonies; despite to a study created by the City of Brno but partially kept, cultivated and reorganized; and rebuilding of the BUT area, which will be opened to the public and communicate with park. The public will obtain an access to nowadays inaccessible and impermeable areas by removing inappropriate buildings and fences, as well as by the modification of the public space and creating one major spine communication realized as an extension of the existing road and which will serve only for the supply, hikers and cyclists. The newly founded BUT complex with its strategic location on the top of Kraví hora hill and next to the observatory will invite large number of people not only from the surroundings but a
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Portable and Miniaturized Separation Techniques Applicable for Food and Biotechnology Analysis
Dvořák, Miloš ; Pospíchal, Jan (referee) ; Bednář,, Petr (referee) ; Rittich, Bohuslav (advisor)
Capillary electrophoresis was used for determination of 6 fractions of caseins. Those fractions were measured in 144 samples of cow’s milk originated from the feeding experiment focused on explanation the influence of the feeding onto casein productions. In this work were separated 6 fraction of caseins first time with total resolution of the peaks. Capillary electrophoresis was applied for determination of short-chain organic acids during fermentation of wine must. It was compared the fermentation of must fermented by different yeast. The difference of profile short-chain organic acids during fermentation were not statistically significant. The once difference was in the utilisation of the malic acid and production of the lactic acid. A portable miniaturized system for medium pressure liquid chromatography was developed. The components were tested and system was used for the isocratic and gradient elution of various analytes (food dyes, parabens). New line of electroluminescent diodes (LEDs) for deep-UV areas of wavelength based on a different materials substrate was characterised. The new line was compared with old line LEDs. The new line LEDs was incorporated in deep-UV absorbance detectors. Detectors were characterised and tested for a detection various analytes in modes flow injection analysis and chromatography separation. First time was characterised this new line of the LEDs and the origin of the parasitic emission band produced by deep-UV LEDs light sources was explained. This origin is given by disturbances of a materials substrates. This work is a contribution for an advance of low-cost and portable systems and detection devices in the field of analytical chemistry.
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Kraví hora - BUT Family silver
Šagátová, Patrícia ; Dvořák, Miloš (referee) ; Nový, Alois (advisor)
The diploma thesis deals with design of the polyfunctional center and the whole complex for the Brno University of Technology, which would serve for its representation. The land is in Brno on Kraví hora and its location has huge potential. However, the area is not used by the university, so the existing buildings are in poor condition. Therefore, the aim of the proposal is to find a suitable use of the land that would meet the university's requirements together with the city's intentions.
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Kraví hora - BUT Family silver
Peštálová, Lucie ; Dvořák, Miloš (referee) ; Nový, Alois (advisor)
The subject of the diploma thesis is to design a project study and a building program that would become a compromise between the requirements of the city of Brno and BUT in the area of Kraví hora in the Brno city center. The city of Brno would like to transform the area into a public park. But BUT considers it as its "family silver" because they realize its extraordinary value. Options of a constructions are really limited because of many area regulations. My proposal counts on the demolition of all existing buildings in the area, respects property area relations , protection zone, continuity with existing traffic roads, height regulation, imaginary raster of surrounding buildings, slope of terrain and orientation to the cardinal points. My suggest represents a study of five groups of objects which creating a rectangular raster. As a compromise solution, I chose the presentation and promotional areas of the individual faculties of BUT for the content of the buildings. Faculties have spaces for hobby groups for the public comprise all age groups included. I also designed the BUT Museum of History, a multimedia hall, a cafeteria, studios, a student leisure center and an observation tower with a climbing wall.
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Coupling of micro-electromembrane extraction to transient capillary isotachophoresis for the analysis of drugs in biological samples
Lučaj, Martin ; Pospíchal,, Jan (referee) ; Dvořák, Miloš (advisor)
The diploma thesis is focused on the development of in-line micro-electromembrane extraction (EME) coupled to capillary electrophoresis (CE) for the analysis of selected drugs in body fluids. Up to now, direct coupling of EME to CE has been demonstrated on diluted river samples only [1]. Although the published set-up has been implemented within a commercial CE it suffers from several drawbacks that can have a negative impact on the analysis of samples with higher complexity. The instrumental arrangement presented in this thesis eliminates these deficiencies. The experimental part is based on the optimization of fundamental extraction and separation conditions for the analysis of model basic drugs (nortriptyline, haloperidol, loperamide) with the use of transient isotachophoresis (tITP) principle. The extraction conditions were optimized for electro-driven transport of basic analytes from complex matrices (urine) through free liquid membrane followed by injection step utilized by electrokinetic supercharging (EKS), which focused target analytes into the CE capillary. Optimized conditions have been applied on blood in the form of dry blood spots, which are highly attractive samples in the current clinical analysis. The repeatability of the measurements was
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