National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Mechanism of cardioprotection elicited by cold acclimation
Csomová, Martina ; Žurmanová, Jitka (advisor) ; Holzerová, Kristýna (referee)
Myocardial infarction is the most common cause of death and disability worldwide. The term myocardial infarction refers to the death of cardiac cells, cardiomyocytes, caused by ischaemia resulting from a perfusion imbalance between the supply and demand for oxygen and nutrients. Recently, cold acclimatization has been found to reduce infarct size after ischemia/reperfusion (I/R) injury, but the mechanism is not fully clarified. Cold acclimatization elicits a thermoregulatory response by activating nontalamic thymogenesis associated with brown adipose tissue (BAT) activation. Cold-stimulated adrenergic signaling increases the expression of mitochondrial uncoupling protein 1 (UCP1), a key factor for heat production. Activated BAT increases metabolic turnover and thus likely contributes to the protection of the heart from myocardial infarction. In this work, I simulate I/R injury by establishing a hypoxic- reoxygenation (H/R) protocol in isolated adult cardiomyocytes. The aim of this work was to introduce different approaches to assess the viability of isolated cardiomyocytes and compare their utility, to investigate the effect of different cold adaptations on cardiomyocyte viability during the H/R protocol using the Cytation 5 multidetector, and subsequently to determine the effect of inhibitors of...
Maturation of brown adipose tissue
Csomová, Martina ; Žurmanová, Jitka (advisor) ; Mančíková, Andrea (referee)
Brown adipose tissue (BAT) is activated under the influence of the adrenergic system and thyroid hormones in response to a cold stimulus. It's main task is to cover the body heat loss and maintain the stability of the internal environment . BAT is in most cases found in new-borns accounting for 5% of the whole body. Cells found in BAT contain a high number of mitochondria with high respiratory capacity but low ATP-synthase activity which allows the production of heat instead of ATP in process of glucose oxidation. The result is non-shivering thermogenesis. The task of my work is to summarize the current knowledge about the development of BAT and to point out the markers for its characterization. The work will also include differences in adipose tissue maturation in selected model organisms. Key words: brown adipose tissue, white adipose tissue, beige adipose tissue, non-shivering thermogenesis, uncoupling protein 1

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