National Repository of Grey Literature 8 records found  Search took 0.00 seconds. 
Intrinsic factors in helper T-cells lineage choice
Andreyeva, Arina ; Neuwirth, Aleš (advisor) ; Chmelař, Jindřich (referee)
The process of clonal expansion of T lymphocytes, or T cells, belongs to the basic characteristics of adaptive immunity. A fundamental role in the immune response is played by the CD4+ T cells which are capable of evolving into the different subtypes (for example Th1 or Tfh) that help other types of cells to effectively eliminate pathogens. Each particular subtype activates different arms of the immune system for the most effective clearance of a particular pathogen. The way how the pathogen will be eliminated depends on the type of infection. This thesis aims to analyze relevant literature and known facts about factors that influence functional T-cell differentiation. This thesis will be mainly focused on the question of how much the T-cell receptor's structure or antigen affinity plays a role in this decision- making process. Another point of interest is the capability of T cells from one clone to produce different T helper cell subtypes, or they are preferentially biased towards a single differentiation pathway. Key words: adaptive immunity, CD4+ T cells, TCR, infections, differentiation
Identification, expression, and characterization of Phlebotomus orientalis salivary antigens
Šíma, Michal ; Kolářová, Iva (advisor) ; Gomes, Regis (referee) ; Chmelař, Jindřich (referee)
Sand flies (Diptera: Psychodidae) are vectors of Leishmania spp. (Kinetoplastida: Trypanosomatidae), medically and veterinary important parasites causing leishmaniasis. These protozoans are inoculated into the host during blood-feeding together with the vector saliva. Salivary components are crucial for sand fly female blood-sucking but also play a major role in the initial stage of parasite development. It was demonstrated that this rich mixture of proteins performs different functions after its injection into the host like hydrolyzing ATP and ADP, binding biogenic amines, and others which inhibits the haemostatic reaction and modulate the immune system thus enables the successful feeding. So far, cDNA salivary transcriptomes have been constructed for twelve sand fly species and we added another one - Phlebotomus orientalis, the main vector of Leishmania donovani, a causative agent of human visceral leishmaniasis in East African countries. We described members of thirteen protein families which corresponded with previously published salivary cDNA libraries of other sand fly species and showed the main salivary antigens using sera of experimentally bitten mice and naturally exposed dogs. Specific IgG antibody response is induced against sand fly salivary proteins in repeatedly bitten hosts....
Comparison and characterization of salivary proteins from Sergentomyia and Phlebotomus sand flies
Polanská, Nikola ; Volf, Petr (advisor) ; Martin-Martin, Ines (referee) ; Chmelař, Jindřich (referee)
Sand flies (Diptera, Phlebotominae) are small biting insects and vectors of Leishmania spp. which cause medically and veterinary important disease - leishmaniasis. During the piercing of the host skin, sand fly females inject saliva to facilitate the blood feeding. The sand fly saliva is composed of many bioactive molecules which were shown to possess anti-inflammatory and anti-haemostatic functions. The saliva affects host's immunity in the bite site and consequently enhances the survival and development of transmitted pathogens. Most of the studies focus on salivary proteins and enzymes of sand flies belonging to Phlebotomus and Lutzomyia genera, while salivary proteins from sand flies of the third genus Sergentomyia were neglected so far. In this thesis we focused on comparison of salivary proteins from two Phlebotomus species, namely Phlebotomus perniciosus and Phlebotomus orientalis, and Sergentomyia schwetzi. These sand fly species differ not only by the ecology and geographical distribution but also by host preferences. Both Phlebotomus species prefer large or medium-size mammals as the bloodmeal source, particularly rabbits, hares and dogs for P. perniciosus and cattle, goats, sheep and humans for P. orientalis. Contrarily, Sergentomyia sand flies are known for preferred feeding on reptiles...
Identification, expression, and characterization of Phlebotomus orientalis salivary antigens
Šíma, Michal ; Kolářová, Iva (advisor) ; Gomes, Regis (referee) ; Chmelař, Jindřich (referee)
Sand flies (Diptera: Psychodidae) are vectors of Leishmania spp. (Kinetoplastida: Trypanosomatidae), medically and veterinary important parasites causing leishmaniasis. These protozoans are inoculated into the host during blood-feeding together with the vector saliva. Salivary components are crucial for sand fly female blood-sucking but also play a major role in the initial stage of parasite development. It was demonstrated that this rich mixture of proteins performs different functions after its injection into the host like hydrolyzing ATP and ADP, binding biogenic amines, and others which inhibits the haemostatic reaction and modulate the immune system thus enables the successful feeding. So far, cDNA salivary transcriptomes have been constructed for twelve sand fly species and we added another one - Phlebotomus orientalis, the main vector of Leishmania donovani, a causative agent of human visceral leishmaniasis in East African countries. We described members of thirteen protein families which corresponded with previously published salivary cDNA libraries of other sand fly species and showed the main salivary antigens using sera of experimentally bitten mice and naturally exposed dogs. Specific IgG antibody response is induced against sand fly salivary proteins in repeatedly bitten hosts....
Transcriptomic and functional analysis of salivary proteins from the tick \kur{Ixodes ricinus}
CHMELAŘ, Jindřich
This thesis was focused on the identification and characterization of the salivary proteins from Ixodes ricinus, the European vector of Lyme disease and tick-borne encephalitis causative agents. In the first part of this work, the the transcriptomic approach was used in order to identify and describe I. ricinus salivary proteins. The second part is dealing with functional and structural characterization of the salivary protein named IRS-2 (I. ricinus serpin-2).

See also: similar author names
1 CHMELAŘ, Josef
4 Chmelař, Jakub
7 Chmelař, Jan
2 Chmelař, Jaroslav
4 Chmelař, Jindřich
10 Chmelař, Jiří
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