|
|
Vliv zdroje selenu na antioxidační potenciál laboratorních potkanů
Buriánková, Markéta
Buriánková M.: Influence of the selenium source on the antioxidant potential of laboratory rats. Diploma thesis, MENDELU, Brno, 90 p. The aim of this thesis was to experimentally determine influence of various forms (nanoselenium, nanoselenium modified by glucose) and levels of selenium (0 mg, 0,02 mg and 0,02 mg of selenium/organism/day + 0,1 mg of glucose/organism/day) on antioxidant potential of model animals - rats. It was used 18 laboratory rat males of outbreeding base Wistar albino, which were separated to three groups. In groups with application of nanoselenium itself the beneficial effect to the final concentration of selenium in plasma and intestines was confirmed (P < 0,05) in contrast to check (without supplementation). In groups treated by nanoselenium modified by glucose had significantly (P < 0,05) beneficial effect on blood with increase by 78,1 % and on liver by 111,3 % in comparison with check. Applied nanoselenium in both groups increased final concentration of selenium in tissues. The supplementation of nanoselenium in other groups of rats did not significantly influenced final concentration of selenium in tissues. Antioxidant activity of tissues significantly (P < 0,05) increased in plasma of group, where the nanoselenium modified by glucose were applied, namely by 83,6 % in comparison with check. It was evaluated by antioxidant methods FR (P < 0,05) and FRAP, where most of the results were without statistical significance (P > 0,05). Both forms of nanoselenium had influence on level of reduced (GSH) and oxidized (GSSG) glutathione. In blood the significant (P < 0,05) increase of GSH level has occurred. In erythrocytes were significantly (P < 0,05) higher both groups with nanoselenium, where higher effect on level of GSH had group with nanoselenium modified by glucose, thereby the oxidation stress of organism was reduced. In liver the significant (P < 0,05) decrease of GSH in group with nanoselenium itself has occurred and in both groups the values of GSSG have significantly (P < 0,05) decreased. The results clearly show that levels of nanoselenium and nanoselenium modified by glucose increased concentration of this element in tissues of animal organism, increased antioxidant activity in plasma and also increased level of GSH in blood and erythrocytes.
|
guest ::
RSS feed.