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Development and Application of High Performance Separation Methods in Connective Tissue Analysis
Braun, Martin
24 Annotation: This work is concerned with the development and application of high performance separation methods suitable for determination of clinically considerable fragments of collagen and elastin as the most important proteins of connective tissues. For their analyses, various chromatographic and electrophoretic methods have been used, including high performance liquid chromatography (HPLC), capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC). This work has aimed at optimization of the developed and modified analytical separation procedures and methods and their utilization for determination of clinically important markers of connective tissue degradation. The key validation parameters of the analytical methods used in this work were evaluated too, which enabled their comparison with other approaches. One of the most important results of this work is thus high sensitivity, precision, repeatability and robustness of the methods which was found in a number of biological sample types. Successful was also very good HPLC separation of the analyzed cross-links in both collagen and elastin tissues. High performance methods are described which have been suggested and optimized for separation and quantitative determination of pentosidine, pyridinolines and...
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Development and Application of High Performance Separation Methods in Connective Tissue Analysis
Braun, Martin ; Štulík, Karel (advisor) ; Pacáková, Věra (referee) ; Samcová, Eva (referee)
24 Annotation: This work is concerned with the development and application of high performance separation methods suitable for determination of clinically considerable fragments of collagen and elastin as the most important proteins of connective tissues. For their analyses, various chromatographic and electrophoretic methods have been used, including high performance liquid chromatography (HPLC), capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC). This work has aimed at optimization of the developed and modified analytical separation procedures and methods and their utilization for determination of clinically important markers of connective tissue degradation. The key validation parameters of the analytical methods used in this work were evaluated too, which enabled their comparison with other approaches. One of the most important results of this work is thus high sensitivity, precision, repeatability and robustness of the methods which was found in a number of biological sample types. Successful was also very good HPLC separation of the analyzed cross-links in both collagen and elastin tissues. High performance methods are described which have been suggested and optimized for separation and quantitative determination of pentosidine, pyridinolines and...
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Development and Application of High Performance Separation Methods in Connective Tissue Analysis
Braun, Martin
24 Annotation: This work is concerned with the development and application of high performance separation methods suitable for determination of clinically considerable fragments of collagen and elastin as the most important proteins of connective tissues. For their analyses, various chromatographic and electrophoretic methods have been used, including high performance liquid chromatography (HPLC), capillary zone electrophoresis (CZE) and micellar electrokinetic capillary chromatography (MEKC). This work has aimed at optimization of the developed and modified analytical separation procedures and methods and their utilization for determination of clinically important markers of connective tissue degradation. The key validation parameters of the analytical methods used in this work were evaluated too, which enabled their comparison with other approaches. One of the most important results of this work is thus high sensitivity, precision, repeatability and robustness of the methods which was found in a number of biological sample types. Successful was also very good HPLC separation of the analyzed cross-links in both collagen and elastin tissues. High performance methods are described which have been suggested and optimized for separation and quantitative determination of pentosidine, pyridinolines and...
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