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The Use of Modern Chromatographic Approaches and Sample Preparation in the Analysis of Biologically Active Substances
Brabcová, Ivana ; Šatínský, Dalibor (advisor) ; Karlíček, Rolf (referee) ; Tolarová, Jitka (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Ivana Brabcová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of thesis: The use of modern chromatographic approaches and sample preparation in the analysis of biologically active substances The presented thesis deals with the modern trends in liquid chromatography and in the analysis of biologically active substances. High performance liquid chromatography (HPLC) is an important analytical tool for separating and quantifying components of mixtures. New trends of HPLC technology tend to development of new stationary phases with better properties. Another trend in analytical chemistry is a miniaturization of the HPLC system and environmentally friendly chromatography. Sample preparation is an essential step in analysis, greatly influencing the reliability and accuracy of analysis. Nowadays, the development is focused on new modern techniques of sample preparation. The developments of these techniques lead up to miniaturization, automatization and preconcentration of target analytes. The theoretical part of the thesis deals with the introduction of modern trends in liquid chromatography and it is divided in several sections. First part describes current trends in fast liquid...
The Use of Modern Chromatographic Approaches and Sample Preparation in the Analysis of Biologically Active Substances
Brabcová, Ivana ; Šatínský, Dalibor (advisor) ; Karlíček, Rolf (referee) ; Tolarová, Jitka (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Mgr. Ivana Brabcová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D. Title of thesis: The use of modern chromatographic approaches and sample preparation in the analysis of biologically active substances The presented thesis deals with the modern trends in liquid chromatography and in the analysis of biologically active substances. High performance liquid chromatography (HPLC) is an important analytical tool for separating and quantifying components of mixtures. New trends of HPLC technology tend to development of new stationary phases with better properties. Another trend in analytical chemistry is a miniaturization of the HPLC system and environmentally friendly chromatography. Sample preparation is an essential step in analysis, greatly influencing the reliability and accuracy of analysis. Nowadays, the development is focused on new modern techniques of sample preparation. The developments of these techniques lead up to miniaturization, automatization and preconcentration of target analytes. The theoretical part of the thesis deals with the introduction of modern trends in liquid chromatography and it is divided in several sections. First part describes current trends in fast liquid...

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2 BRABCOVÁ, Iva
3 Brabcová, Irena
2 Brabcová, Iva
4 Brabcová, Iveta
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