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The Balance of the Nitrogen in Agriculture
Klír, Jan ; Beranová, Jana ; Dědina, Martin ; Wollnerová, Jana
Methodology addressing nitrogen balance issues is a suitable tool for verifying the correctness of agricultural management and shows the relation between nitrogen used in agriculture and its losses in gaseous form to air or in the form of nitrates to surface and groundwater. The assessment of nitrogen balance, greenhouse gas emissions and pollutants is closely linked and therefore requires the quantification of nitrogen sources used in agriculture. Synchronization of input data used to evaluate nitrogen flows in agriculture is essential to increasing the methodological level of reporting the nitrogen balance, greenhouse gas emissions and pollutants for the Czech Republic in terms of the requirements of international organizations.
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Prevention of the occurrence and spread of natural fires in the immediate vicinity\nsurface sources of drinking water, including methods for estimating the consequences\npossible occurrence
Beranová, J. ; Čermák, Petr ; Albert, J. ; Cienciala, Emil ; Zatloukal, V. ; Kudláčková, Lucie ; Novotný, Jan ; Zeman, Evžen ; Kuráž, M. ; Vizina, Adam ; Trnka, Miroslav
The aim of the methodology is to create a pragmatic procedure for assessing the risks of the occurrence and spread of natural fires\nin the immediate vicinity of surface sources of drinking water. Methods for estimation are proposed in the methodology\nconsequences of the occurrence of natural fires on the quality of surface water, the prognosis of the change in the risk of fires\ndue to climate change and the procedure for determining the risk of secondary pollution of reservoirs due to\nchanges in runoff conditions after a natural fire. On this basis, the methodology designs and diversifies\npreventive and operational measures.
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Risk assessment and prediction of natural fires in the immediate vicinity\nsurface sources of drinking water.
Trnka, Miroslav ; Kudláčková, Lucie ; Čermák, P. ; Balek, Jan ; Novotný, Jan ; Homolová, Lucie ; Semerádová, Daniela ; Brovkina, Olga ; Štěpánek, Petr ; Zahradníček, Pavel ; Skalák, Petr ; Bláhová, Monika ; Benáček, Patrik ; Fischer, Milan ; Sedlák, Pavel ; Janouš, Dalibor ; Žalud, Zdeněk ; Marek, Michal V. ; Možný, M. ; Hájková, L. ; Chuchma, F. ; Knozová, G. ; Beranová, J. ; Zatloukal, V. ; Albert, J. ; Mašková, R. ; Cienciala, E. ; Vizina, A. ; Nesládková, M. ; Melišová, E. ; Hanel, M.
The methodology formulates a procedure for assessing the risks of the occurrence and spread of natural fires in the immediate vicinity of surface sources of drinking water. The methodology\nproposes methods for estimating the consequences of natural fires on surface water quality, forecasting the change in the risk of fires due to climate change and the procedure for determining the risk of secondary pollution of reservoirs due to changes in run off after a natural fire. On this basis, the methodology proposes and diversifies preventíve and operational measures.The measures were designed in connection to modeling results for the Hadce pilot síte near the Švihov reservoir and the experience with the adverse effects of extensive deforestation on the water quality in the Vranov and Vír reservoirs.
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The Castle of Sokolov and its History
Beranová, Jitka ; Zilynskyj, Bohdan (advisor) ; Vlnas, Vít (referee)
Za hlavní přínos práce považuji shrnutí dosud známých poznatků o zámku v Sokolově a jejich určitou revizi. Zejména studiem archivního materiálu se mi podařilo získat nové poznatky opravující a obohacující dosavadní znalosti. Značnou pozornost jsem věnovala především zámeckým inventářům. Díky tomu bylo možno mimo jiné zpřesnit dataci a průběh klasicistní přestavby, uskutečněné již před rokem 1800, snad na počátku roku 1795, kdy nejprve došlo k rozdělení tzv. velkého sálu a následně jídelny v severním křídle. Stejně tak považuji za přínosné objevení fotografií interiérů zámku, případně jeho nejbližšího okolí, neboť tento materiál k jeho historii nebyl zatím odborně využit. Provedené sondy do rodinné korespondence, spadající zejména do 19. stol., naznačují, jaký vztah měli Nosticové ke zdejší oblasti, kdy zde pobývali či jak trávili čas. Tato korespondence nebyla doposud více využita, badatelé se o ni spíše zajímali ve vztahu k osobnosti Josefa Dobrovského. Zámek v Sokolově stál z hlediska Nosticů poněkud ve stínu jejich paláce v Praze či zámku v Měšicích, nenacházely se zde ani jejich hlavní sbírky, jako např. sbírka mědirytin, obrazů či nostická majorátní knihovna. Do konce 18. stol. sloužil spíše jako správní centrum a členové rodu zde příliš času netrávili. Určitá změna nastala na poč. 19. stol., kdy byl...
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Růst Mycobacterium smegmatis na agarovém médiu a agarovém médiu pokrytém celofánovou folií - morfologická a proteomová studie
Ramaniuk, Volha ; Weiser, Jaroslav (advisor) ; Beranová, Jana (referee)
Biofilm formation is one of the most common bacterial survival strategies. Majority of bacterial species are able to form these three-dimensional structures, including pathogens like Mycobacterium tuberculosis. Representatives of Mycobacterium genus widely occur in the nature, although they can cause serious problems when they appear in medical equipment and artificial replacements of the human body. Non-pathogenic Mycobacterium smegmatis mc2 155 was used as a model organism in our experiments. We investigated morphology of the three- and six-day-old colonies (in fact biofilms) on agar and agar covered with cellophane using Stereo microscope and Scanning Electron Microscope. We found that a type of surface as well as a carbon source has a great influence on the morphology of the M. smegmatis colonies. We isolated proteomes from the agar and cellophane cultures and from planktonic culture. Two-dimensional electrophoresis was used as the main proteomic method. Proteomic data were analyzed using PDQuest software. Then the sets of proteins detected by qualitative and quantitative analyses were compared using Venn diagrams. As a result, we recognized 7 unique proteins that might be specific for recognition and adhesion of bacteria to the cellophane, no unique protein in agar proteome and 46 unique...
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The delta subunit of RNA polymerase from gram positive bacteria
Matějčková, Jitka ; Krásný, Libor (advisor) ; Beranová, Jana (referee)
1 Abstrakt Aby bakteriální buňka přežila neustále se měnící podmínky, musí se na ně adaptovat. Tato adaptace je podmíněna změnou genové exprese. Klíčovým krokem genové exprese je transkripce. Hlavním enzymem bakteriální transkripce je RNA polymerasa (RNAP), což je esenciální vícepodjednotkový enzym. RNAP je nejvíce prostudována u Escherichia coli, modelového organismu gram negativních bakterií. Porovnala jsem E. coli a Bacillus subtilis (zástupce gram pozitivních bakterií) a shrnula jsem rozdíly v RNAP a transkripci. Jejich RNA polymerasy se liší přítomností podjednotky δ u gram pozitivních bakterií. Tato podjednotka zvyšuje promotorovou selektivitu, recykluje jádro RNAP a celkově stimuluje syntézu RNA. Podjednotka δ ovlivňuje sporulaci a virulenci některých bakterií. V této práci jsem shromáždila současné poznatky o jednotlivých částech genové exprese, zejména o regulaci iniciace transkripce a o podjednotce δ RNAP.
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Non-conventional bacterial signaling pathways
Krupička, Jiří ; Branny, Pavel (advisor) ; Beranová, Jana (referee)
Two component systems were traditionally considered as main phosphorylation systems of bacteria involved in cell signalling. Recently, attention focuses increasingly on bacterial eukaryote-like Ser/Thr protein kinases (eSTKs). These protein kinases are structurally similar to their eukaryotic counterparts. Some eSTKs possess additional domains such as extracellular PASTA domains that were discovered in a variety of gram-positive bacteria. It has been proved that these domains can act as sensors for unlinked peptidoglycan fragments. However, majority of environmental signal molecules still remains unknown. eSTKs phosphorylate a broad spectrum of substrates including proteins involved in various cell processes such as virulence, cell wall biosynthesis, cell division, and central and secondary metabolism. Cross talk between eSTKs and two component systems also occurs. In this thesis, the current knowledge about eSTKs and their significant substrates in different bacterial species is discussed.
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Bacterial adhesion to surfaces
Budil, Jakub ; Beranová, Jana (advisor) ; Kuthan, Martin (referee)
Bacterial adhesion is a process of attachment of a planktonic cell to the surface and the necessary step for further biofilm formation. Most bacterial species are capable of biofilm formation. Bacteria within biofilm exhibit increased resistance to antibiotics and other external factors, which makes the eradication of adhered bacteria rather difficult. Bacterial adhesion is very complex process affected by many physical and chemical factors as well as by the adhesive molecules present on the substrate and cell surface. Accurate description of these factors enables to design appropriate modifications of both the substrate and the environment with the aim to fabricate the antiadhesive and antibacterial surfaces or to increase the efficiency in bacteria-based biotechnological processes. Whereas in medical applications the main goal is to decrease bacterial adhesion, the increased adhesion is desirable in certain processes employing bacteria, as is for example the wastewater treatment. This thesis reviews main theories used for prediction of bacterial adhesion, describes the process of adhesion and physical, chemical and biological factors that influence it. It also contains an overview of antiadhesive and antibacterial surfaces. Key words: Bacteria, adhesion, pili, flagellum, antiadhesive and...
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