National Repository of Grey Literature 51 records found  beginprevious42 - 51  jump to record: Search took 0.00 seconds. 
Pokroky v průtokové cytogenetice ječmene
Suchánková, Pavla ; Kubaláková, Marie ; Kovářová, Pavlína ; Bartoš, Jan ; Čihalíková, Jarmila ; Endo, T. R. ; Doležel, Jaroslav
Flow cytogenetics is an attractive tool that facilitates genome mapping. Flow cytometric analysis permits classification of isolated chromosomes according to their DNA content As thousands of chromosomes are analysed in each sample, quantitative detection of structural and numerical chromosome changes is possible. Any chromosome that can be discriminated can also be sorted in large quantities and high purities for subsequent molecular analysis. Unfortunately, only the smallest chromosome 5(1H) can be discriminated and sorted in barley. This is due to similarity in relative DNA content among the chromosomes 1 - 4,6 and 7 (2H - 7H). Cytogenetic stocks facilitate sorting of those translocation chromosomes that differ in DNA content Even if the sorted translocation chromosomes were found useful for mapping DNA sequences to subchromosomal regions, their use remained limited. Here we describe a novel approach that facilitates sorting of any of the 14 barley chromosome arms. The strategy is based on using wheat-barley addition lines carrying barley chromosome arms (telocentric chromosomes). Furthermore, we will be able to sort subarm chromosomal segments that are generated in the wheat-barley chromosome addition lines by the gametocidal system. These advances make flow cytogenetics an attractive tool that can simplify barley genome mapping. A possibility to purify large quantities of individual chromosome arms and their segments opens avenues for targeted isolation of low-copy "genie" sequences, preparation of specific probes for screening EST arrays, HAPPY mapping, and construction of chromosome arm-specific BAG libraries.
Rozdělení velkých genomů čeledi Triticeae tříděných chromozomů
Doležel, Jaroslav ; Kubaláková, Marie ; Suchánková, Pavla ; Šafář, Jan ; Janda, Jaroslav ; Kovářová, Pavlína ; Bartoš, Jan ; Čihalíková, Jarmila ; Šimková, Hana
Nuclear genomes of some Triticeae species are characterized by large size and prevalence of repetitive DNA sequences. These features hamper their physical mapping and gene cloning. Purification of individual chromosomes by flow cytometry can simplify these tasks by providing small and defined genome fractions. This lecture reviews the development of the methodology and its potential for genome mapping in barley, rye and wheat Due to small differences in relative DNA content, only one chromosome type can be discriminated and sorted in each of the three species. Cytogenetic stocks facilitate separation of other parts of the genomes as individual chromosomes, translocation chromosomes and chromosome arms. Chromosome analysis by flow cytometry permits quantitative detection of structural and numerical chromosome changes. Chromosomes sorted onto microscopic slides have been used for discovery of rare structural changes and for high-resolution cytogenetic mapping using FISH. The use of sorted chromosomes for HAPPY mapping, targeted isolation of low copy "genie" sequences, and high-throughput physical mapping of ESTs on microarrays are attractive options. As millions of chromosomes with intact DNA may be sorted, construction of BAC libraries is possible. Subgenomic, chromosome-specific and chromosome arm-specific BAC libraries have already been produced in wheat and represent unique resources for genomics of cereals.
Support of bachelor and master thesis couching
Winkler, Jakub ; Pavlíček, Luboš (advisor) ; Bartoš, Jan (referee)
Today, procedure of creating bachelor and master thesis suffers from many problems. Amongst them the main issue would consist of absence of central storage ability connected to creating and couching the thesis. In practice that would mean information about records from consultation, comments to thesis etc. are to be lost and neither student, nor his thesis couch can take a look to the history of thesis progress. Furthermore, there is a lack of date registration for consultations and deadlines of handing out thesis. As a consequence, both efficiency and quality of final thesis are decreased by such poor management. As a solution, support of whole thesis procedure with software tool is suggested. There are many options of such software solutions, however only one is the most suitable and that is exactly what this thesis is trying to find. Thesis formulates requirements for such software application, after the analysis of current situation and identification of core issues. Application should bring improvement of the situation and at the same time should be users, operational and financially acceptable. From wide spectrum of available solutions, only those which had fulfilled basic conditions were chosen. Basic conditions such as: users interface in Czech language, saving documents availability and large capacity of users accounts. Final group of potential solutions was put through detail analysis for support utility for thesis couching. This analysis finally shows the most suitable solution. This bachelor thesis brings analysis of utility for commonly available web application or hosted applications for bachelor and diploma thesis couching. The implementation could be done quickly without further problems, thanks to the undemanding IT and financial requirements of software application. After finding the most suitable solution, this thesis shows how large scale of efficiency Microsoft SharePoint Services 3.0 brings. Furthermore, at the end of this thesis, many more options for using this application are shown.
Analýza a návrh webového rozhraní pro uživatele služeb typu Document delivery jako součást Analýzy implementace DDS do AKS NPKK
Bartoš, Jan ; Švastová, Zuzana (advisor) ; Vronková, Lada (referee)
Práce popisuje vytvoření návrhu nového webovského rozhraní e-PK pro nově vytvářený modul ?document delivery? automatizovaného knihovního systému KP-WIN SQL. Návrh byl vypracován na základě analýzy stávajícího rozhraní e-PK a porovnání e-PK s rozhraním Virtuální polytechnické knihovny. Dále byly zakomponovány návrhy, potřeby a přání servisního centra e-PK za využití dynamicky generovaných stránek a přístupu k databázi.
Změna marketingové strategie firmy NITEX Tábor, s. r. o.
Bartoš, Jan ; Pevná, Jana (advisor)
Charakteristika produktového portfolia, objasnění nové marketingové strategie.
Strukturální fondy EU a příklad realizace operačního programu Průmysl a podnikání
Ulrych, Michal ; Adámková, Vlasta (advisor) ; Bartoš, Ján (referee)
Strukturální fondy EU v kontextu České republiky, se zahrnutím předvstupích nástrojů. Porovnání zkráceného období 2004-2006 s aktuálním obdobím 2007-2013. Specializace na Operační program Průmysl a podnikání a příklad realizace projektu Technologického centra Hradec Králové.
Management podniku a daně
Bartoš, Jan Bc. ; Dvořák, Jiří (advisor) ; Zmrhalová, Alena (referee)
DP popisuje vliv daňové zátěže a vliv případných změn daňového systému na konkurenceschopnost podniku. Management podniku řeší vysokou daňovou zátěž třemi způsoby efektivní daňové optimalizace: 1. vyplácení nižších mezd vlastníkům společnosti 2. vyplácení cestovních náhrad namísto zakoupení automobilů do firemního vlastnictví 3. nákup dlouhodobého hmotného majetku v pořizovací ceně do 40 tisíc Kč.

National Repository of Grey Literature : 51 records found   beginprevious42 - 51  jump to record:
See also: similar author names
34 BARTOŠ, Jan
15 BARTOŠ, Jiří
4 Bartoš, Jakub
1 Bartoš, Jan Bc.
2 Bartoš, Jaromír
1 Bartoš, Jaroslav
15 Bartoš, Jiří
1 Bartoš, Jonáš
7 Bartoš, Josef
1 Bartoš, Julie
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